Targeting Topics

Anti-DBH-SAP, Cat. #IT-03[Dopamine Beta Hydroxylase antibody conjugated to Saporin] Objective: To investigate the role of hindbrain catecholamine neuron pathways and their contribution to long-term energy homeostasis by controlling obesogenic sensitivity to a high-fat, high sucrose choice diet. Summary: The authors show that catecholamine neurons (primarily in the VLM and NTS) convey essential feedback signals to […]

Streptavidin-ZAP[Streptavidin conjugated to Saporin](Cat. #IT-27) Objective: To determine if an immunotoxin cocktail targeted to multiple epitopes has synergistic effects on low expression level cells, which would expand the applicable range of immunotoxin therapy for cancer. Summary: The combination of immunotoxins with different mechanisms of action in an antibody cocktail will increase cytotoxic activities and decrease

NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP) Objective: To determine if differentiated neural crest cells are promising supporting cell candidates to aid in peripheral nerve repair. Summary: In this study, neural crest cells were differentiated from human embryonic stem cells. NGFR (mouse monoclonal, 1:100) Immunocytochemistry.  The specificity of NGFR antibody was validated by immunocytochemical

Congratulations to the Selbo laboratory for their recent publication using Anti-CD105-SAP. This targeted toxin was previously part of the BETA program. As first to publish using this product, Selbo’s group receives a $500 product credit and applause for their great work. 5-Fu Resistant Emt-Like Pancreatic Cancer Cells Are Hypersensitive to Photochemical Internalization of the Novel

Aptamers are small, synthetic, single-stranded nucleic acids that fold into unique secondary structures. Unlike peptides and monoclonal antibodies, they are essentially non-immunogenic even when administered in excess amounts. The publications below explain how biotinylated aptamers combined with Streptavidin-conjugated saporin (Streptavidin-ZAP) can be used to screen aptamers for their ability to internalize into a specific cell

Characterization of the First Fully Human Anti-Tem1 Scfv in Models of Solid Tumor Imaging and Immunotoxin-Based Therapy. Yuan, X, Yang, M, Chen, X, et al. (2018). Cancer Immunol Immunother 2018/01/10. Streptavidin-ZAP (Cat. #IT-27) Objective: ScFv78 was conjugated with the ribosome-inactivating protein saporin (Streptavidin-ZAP) to evaluate whether scFv78 may be used as a vehicle for theTEM1-targeted

2018 starts off with 3 new publications using ATS products. Check out these articles and take advantage of special promotions for each of the products used. Melanopsin Retinal Ganglion Cells Are Not Labeled in Thy-1yfp-16 Transgenic Mice. Grillo, SL, & Stella, SL, Jr. (2018). Neuroreport, 29 (2):118-122. 2017/12/19. Objective: to determine whether mRGCs are labeled

To determine which subtype of nociceptor, peptidergic or non-peptidergic, is involved in the mechanical hyperalgesia induced by LMWH (low molecular weight hyaluronan), rats were pretreated with neurotoxins previously shown to selectively deplete these two types of sensory neurons:  Isolectin B4 (IB4)-saporin (SAP), which acts on IB4-positive, non-peptidergic neurons (Cat. #IT-10 IB4-SAP), and substance P-saporin (Cat.

To examine the role of NMBR+ dorsal horn neurons in itch and pain transmission, the authors treated mice with NMB-SAP (Cat. #IT-70; 2–3 μg, i.t.) at which no side effects were observed. NMB-SAP treated mice barely showed scratching behaviors, whereas control mice exhibited NMB-induced scratching with a rapid onset of scratching responses. These data demonstrate

Since 2000, Advanced Targeting Systems has presented an award each year at the Society for Neuroscience meeting for the best Poster of the Year using ATS targeting tools. This year the competition was fierce and some very exciting science was presented. Also, a big congratulations to the winner of the drawing for a $500 product