Kristen Hartman – Page 235 – Advanced Targeting Systems

Spinal mu-opioid receptor-expressing dorsal horn neurons: role in nociception and morphine antinociception.

Kline IV RH, Wiley RG (2008) Spinal mu-opioid receptor-expressing dorsal horn neurons: role in nociception and morphine antinociception. J Neurosci 28:904-913. doi: 10.1523/JNEUROSCI.4452-07.2008 Summary: The authors used Dermorphin-SAP (Cat. #IT-12) to investigate the function of spinal cord mu-opioid receptor (MOR)-expressing dorsal horn neurons in nociception and morphine analgesia. Rats were treated with 500 ng intrathecal […]

Spinal mu-opioid receptor-expressing dorsal horn neurons: role in nociception and morphine antinociception. Read More »

Antidepressant-induced internalization of the serotonin transporter in serotonergic neurons.

Kristen Hartman

Lau T, Horschitz S, Berger S, Bartsch D, Schloss P (2008) Antidepressant-induced internalization of the serotonin transporter in serotonergic neurons. FASEB J 22(6):1702-1714. doi: 10.1096/fj.07-095471 PMID: 18216289 Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09)

Antidepressant-induced internalization of the serotonin transporter in serotonergic neurons. Read More »

Visualizing cold spots: TRPM8-expressing sensory neurons and their projections.

Kristen Hartman

Dhaka A, Earley TJ, Watson J, Patapoutian A (2008) Visualizing cold spots: TRPM8-expressing sensory neurons and their projections. J Neurosci 28(3):566-575. doi: 10.1523/JNEUROSCI.3976-07.2008 PMID: 18199758 Related Products: Antibody to NK-1 Receptor (Cat. #AB-N04)

Visualizing cold spots: TRPM8-expressing sensory neurons and their projections. Read More »

Effects of ibotenate and 192IgG-saporin lesions of the nucleus basalis magnocellularis/substantia innominata on spontaneous sleep and wake states and on recovery sleep after sleep deprivation in rats.

Kristen Hartman

Kaur S, Junek A, Black MA, Semba K (2008) Effects of ibotenate and 192IgG-saporin lesions of the nucleus basalis magnocellularis/substantia innominata on spontaneous sleep and wake states and on recovery sleep after sleep deprivation in rats. J Neurosci 28:491-504. doi: 10.1523/JNEUROSCI.1585-07.2008 Objective: To examine the role of this area of the brain in several facets

Effects of ibotenate and 192IgG-saporin lesions of the nucleus basalis magnocellularis/substantia innominata on spontaneous sleep and wake states and on recovery sleep after sleep deprivation in rats. Read More »

Targeting Tools: Stimulate Instead of Eliminate

News Tools / Kristen Hartman

SP-CTA (Cat. #IT-39) is an exciting new tool for use in the research of neurokinin (NK-1) receptor-expressing cells of the central nervous system. A conjugate of the substance P molecule and the catalytic subunit of Cholera toxin, this product can be used very effectively in vivo for increasing sensitization of these neuronal cells. Selectively targeting

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Targeting Tools: Anti-AChR

News Tools / Kristen Hartman

Species Reactivity: Human, Rat, Mouse, Rabbit Anti-AChR (Cat. #AB-N36) binds with high affinity to the AChR subtype with 59 kDa ACh-binding subunits, but with considerably lower affinity to the AChR subtype with 75 kDa subunits. Anti-AChR binds to the main immunogenic region on α1 subunits of muscle-type AChRs. Reference 1. Schoepfer R, Halvorsen SW, Conroy

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Targeting Tools: Targeting FGF Receptors

News Tools / Kristen Hartman

FGF-2, or basic fibroblast growth factor, binds all of the FGF receptors with high affinity. We have used this molecule to produce FGF-SAP (Cat. #IT-38), which has a healthy experimental publication record (“FGF” and “saporin” in PubMed: 25 hits). It has been used to clean primary cultures of fibroblasts.[1] It was important in determining the

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Reducing Agents

Kristen Hartman

Q: I have a question about what solutions might be incompatible with the conjugated saporins. We have done an experiment where we injected a mixture of saporin conjugate (same batch we’ve used in previous studies here) and a cocktail of 5,7-dihydroxytryptamine and 6-hydroxydopamine (in 0.1% ascorbic acid) to try to deplete multiple neurotransmitters. The way

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Secondary Conjugate Flow Cytometry

Kristen Hartman

Q: I’m using your secondary conjugate Mab-ZAP (Cat. #IT-04) and it’s not killing my cells. I’m not following the protocol on the data sheet. I’m doing flow cytometry. I have 70,000 cells per well. I mix Mab-ZAP with my primary antibody and add it. When I count the cells, there is no decrease. My cells grow

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Featured Article: Role of medial septal GABAergic neurons in learning and extinction: Effects of the novel GABA immunotoxin GAT1-SAP

Kristen Hartman

Pang KCH, Jiao X, Servatius RJ (2008) Featured Article: Role of medial septal GABAergic neurons in learning and extinction: Effects of the novel GABA immunotoxin GAT1-SAP. Targeting Trends 9(1) Related Products: GAT1-SAP (Cat. #IT-32) Read the featured article in Targeting Trends.

Featured Article: Role of medial septal GABAergic neurons in learning and extinction: Effects of the novel GABA immunotoxin GAT1-SAP Read More »

Author name: Kristen Hartman