Q: Regarding your HRP-labeled Antibody to p53 (Cat. #AB-236), your data sheet states the antigen was 15-40 a.a. Does this a.a. count from N-terminus? A: There was an error on the data sheet which has since been corrected. The AB-236 immunogen is a KLH-conjugated peptide corresponding to amino acids 6-45. The numbering does start from […]
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Q: We are using SSP-SAP (Cat. #IT-11) to lesion NK-1r-bearing neurons. I have the conjugate diluted in solution and was wondering whether or not it is okay to leave it out at room temperature overnight? I would like to use an aliquot over a period of two days. Also, would it be okay to combine
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Khan AM, Rapp KL, Ponzio TA, Sanchez-Watts G, Watts AG (2009) Featured Article: Deletion of catecholaminergic neurons by anti-DBH-saporin disrupts hypothalamic MAP kinase and CREB activation. Targeting Trends 10(2) Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18) Read the featured article in Targeting Trends. See Also: Khan AM et al. Stimulus-, circuit- and intracellular-level
Q: Could you please tell me if the Somatostatin 14 antibody (Cat. #AB-04) will also pick up the Somatostatin 28 residue? A: Yes, it will, because they share the sequence of SS14. However, the Somatostatin-28 antibody (Cat. #AB-05) will not see Somatostatin-14. Related: Anti-Somatostatin-14 (Cat. #AB-04), Anti-Somatostatin-28 (Cat. #AB-05)
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Contributed by Arshad M. Khan, Kimberly L. Rapp, Todd A. Ponzio, Graciela Sanchez-Watts and Alan G. Watts; Dept. of Biological Sciences and Neuroscience Research Institute, University of Southern California, Los Angeles, CA; and Laboratory for Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD The brain has evolved adaptive mechanisms for
Botly LC, De Rosa E (2009) Cholinergic deafferentation of the neocortex using 192 IgG-saporin impairs feature binding in rats. J Neurosci 29:4120-4130. doi: 10.1523/JNEUROSCI.0654-09.2009 Summary: It has been hypothesized that the nucleus basalis magnocellularis (NBM) is the source of cholinergic input to the neocortex that is responsible for incorporating different features of an object into
Montero Dominguez M, Gonzalez B, Zimmer J (2009) Neuroprotective effects of the anti-inflammatory compound triflusal on ischemia-like neurodegeneration in mouse hippocampal slice cultures occur independent of microglia. Exp Neurol 218:11-23. doi: 10.1016/j.expneurol.2009.03.023 Summary: In this work the authors looked to clarify the role of microglia in an experimental stroke model. Hippocampal slices were subject to
Lai CY, Wiethoff CM, Kickhoefer VA, Rome LH, Nemerow GR (2009) Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer. ACS Nano 3(3):691-699. doi: 10.1021/nn8008504 PMID: 19226129 Objective: To explore the feasibility of improving vault penetration into target cell. Summary: The membrane lytic activity of the protein VI (pVI) domain was
Lujan HL, Palani G, Chen Y, Peduzzi JD, Dicarlo SE (2009) Targeted ablation of cardiac sympathetic neurons reduces resting, reflex and exercise-induced sympathetic activation in conscious rats. Am J Physiol Heart Circ Physiol 296:H1305-H1311. doi: 10.1152/ajpheart.00095.2009 Summary: This work examines the capability of CTB-SAP (Cat. #IT-14) to eliminate cardiac sympathetic neurons. The right and left
Tronson NC, Schrick C, Guzman YF, Huh KH, Srivastava DP, Penzes P, Guedea AL, Gao C, Radulovic J (2009) Segregated populations of hippocampal principal CA1 neurons mediating conditioning and extinction of contextual fear. J Neurosci 29:3387-3394. doi: 10.1523/JNEUROSCI.5619-08.2009 Summary: This work examines what cell groups are responsible for controlling contextual fear. 180 ng of mu