Lai CY, Wiethoff CM, Kickhoefer VA, Rome LH, Nemerow GR (2009) Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer. ACS Nano 3(3):691-699. doi: 10.1021/nn8008504 PMID: 19226129
Objective: To explore the feasibility of improving vault penetration into target cell.
Summary: The membrane lytic activity of the protein VI (pVI) domain was retained upon incorporation into vault particles. Moreover, internalization of vault-pVI complexes into murine macrophages promoted co-delivery of a soluble ribotoxin or a cDNA plasmid encoding GFP.
Usage: Saporin was used in cell membrane penetration assays. Concentration varied from 1.65 ×10-7M to 8.25×10-9M in two-fold dilutions.
Related Products: Saporin (Cat. #PR-01)