News from Science: Nppb in Itch / CRH antibody cross-reactivity

Cat. #IT-69 Nppb-SAP

Meng, Qing-Tao and Liu, Xian-Yu and Liu, Xue-Ting and Barry, Devin M. and Jin, Hua and Yang, Qianyi and Sun, Yu and Wan, Li and Jin, Jin-Hua and Munanairi, Admire and Kim, Ray and Yin, Jun and Tao, Ailin and Chen, Zhou-Feng, Cross-Talk between Distinct Receptors Shapes Itch Behavior in the Spinal Cord (September 14, 2018). Available at

Summary: Consistently, BNP-saporin ablated spinal Npr1 and Npr3 neurons and impaired histamine-, but not CQ-evoked, itch. Thus, the findings identify the role of BNP-NPRC signaling in modulation of histamine-evoked itch via NPRC-NMBR cross-talk independent of GRP-GRPR signaling. Our studies reveal distinct modes of action for bombesin-related peptides and NP in itch transmission.

Cat. #AB-02 Anti-CRH

Immunohistochemical Detection of Prolactin-Releasing Peptide2 in the Brain of the Inshore Hagfish Eptatretus Burgeri. Amano M, Amiya N, Yamamoto N, Osugi T, & Tsutsui K. (2018). Gen Comp Endocrinol Epub 17 December 2018.

Objective: To identify Prolactin-releasing peptide2 (PrRP2) in hagfish.
Summary: The study demonstrates, for the first time, that PrRP2 is expressed in the brain of inshore hagfish. The restricted distribution of PrRP2-ir fibers in the HYinf suggests that PrRP2 does not directly regulate the pituitary gland, but regulates the function of the HYinf where PQRFa and CRH are expressed.
Dose:  Immunohistochemistry (1:1000). The authors clarified the cross-reactivity of the antibody against other CRH family peptides, such as urocortin-I, II, III, urotensin-I, and sauvagine: the cross-reactivity of anti-CRH against CRH family peptides was found to be less than 0.01%, indicating the specificity of the antibody.

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