New Publications – Retinal Ganglion Cells and Cerebellar Purkinje Cells

Two publications out this week using ATS antibodies:

Anti-Melanopsin (Cat. #AB-N38) and Anti-Metabotropic Glutamate Receptor 2 (mGluR2; Cat. #AB-N32)

Sabbah S et al.  Synaptic circuits for irradiance coding by intrinsically photosensitive retinal ganglion cells. (2018) bioRxiv  doi:

Objective:  To explore the synaptic networks responsible for the unique capacity of intrinsically photosensitive retinal ganglion cells (ipRGCs) to encode overall light intensity. This luminance signal is crucial for circadian, pupillary and related reflexive responses light.
Summary:  Most ipRGCs sample from all bipolar terminals costratifying with their dendrites, but M1 cells avoid all OFF bipolar input and accept only ectopic ribbon synapses from ON cone bipolar axonal shafts. These monad synapses are equipped with as many as a dozen ribbons and only one postsynaptic process.
Dose: Immunohistochemistry of retina – after recording, retinas were fixed and counterstained with rabbit anti-melanopsin (1:1000)  to enhance the fluorescence of the GFP-based GCaMP6f indicator.

Kamath P, & Chen A. Myocyte Enhancer Factor 2c Regulates Dendritic Complexity and Connectivity of Cerebellar Purkinje Cells. (2018). Molecular Neurobiology

Objective:  To investigate the roles of Mef2c in cerebellar Purkinje cells during the first three weeks of postnatal development.
Summary:  Mef2c haploinsufficiency is implicated in behavioral deficits related to autism, schizophrenia, and intellectual disability.  Results reveal the specific expression and functional relevance of Mef2c in developing Purkinje cells and offer insight to how disruption of the expression of Mef2c in a GABAergic neuronal subtype may lead to pathogenesis of cerebellar-associated disorders.
Dose: Immunohistochemistry – mouse anti-mGluR2 (1:1500)