ATS is pleased to re-release a product and kit specific for the Orexin 2 receptor (OX2R). The Orexin 1 and Orexin 2 receptors are found in the perifornical area/latero-posterior hypothalamus, and projections from this area cover much of the brain. These receptors have been implicated in various neurophysiological and neuropsychological disorders such as narcolepsy, insomnia, drug addiction, anxiety, and migraine headaches. The Orexin-B-SAP conjugate consists of the rat/mouse-specific orexin-B peptide conjugated to Saporin. Orexin-B binds to OX2R with approximately 5X greater affinity than to OX1R (Fig. 2).
Orexin-B-SAP (Cat. #IT-20) is highly specific for cells that express OX2R. Instead of spending precious time and money producing an OX2R knockout animal, you can use Orexin-B-SAP to specifically eliminate cells that express OX2R (Fig. 1). Orexin-B-SAP also allows you to study the behavioral effects before and after treatment and subsequent elimination of OX2R expressing cells. The specificity and efficacy of Orexin-B-SAP has recently been reported in the published work of Schwartz et al.
See also Toolbox: Orexin Receptor Antibody Conjugates.
- Schwartz MD, Nguyen AT, Warrier DR, Palmerston JB, Thomas AM, Morairty SR, Neylan TC, Kilduff TS. (2016) Locus Coeruleus and Tuberomammillary Nuclei Ablations Attenuate Hypocretin/Orexin Antagonist-Mediated REM Sleep. eNeuro 3(2) PMID: 27022631.