09q2

Targeting Trends 2009 Newsletter

2009, issue 1: Selective deletion of CD8+ T cells by saporin-coupled MHC class I tetramers / by Hess PR, Buntzman AS, Murray SL, Young EF, Frelinger JA / featuring Streptavidin-ZAP (Cat. #IT-27), FITC-labeled SAP antibody (Cat. #FL-02) SFN Poster of the Year Targeting Talk: Anti-IgM-ZAP (Anti-M-ZAP, Cat. #IT-30) 2009, issue 2: Deletion of Catecholaminergic Neurons by Anti-DBH-Saporin Disrupts Hypothalamic MAP Kinase …

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Targeting Tools: CTB-SAP

CTB-SAP (Cat. #IT-14) is a conjugate between the cell-binding component of cholera toxin (the B chain) and saporin. CTB binds to GM1 (monosialotetrahexosylganglioside), which is present on the surface of different neurons. It has been suggested to be involved in many problems (besides the most famous in the gut: cholera) of neuronal systems: Parkinson’s, motor …

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Targeting Tools: Hug-M-ZAP

This secondary conjugate (molecular weight 210 kDa) recognizes YOUR human monoclonal IgM antibody. Hug-M-ZAP (IT-43) is a chemical conjugate of affinity-purified goat anti-human IgM and the ribosome-inactivating protein, saporin. Hug-M- ZAP uses your primary human monoclonal IgM antibody to target and eliminate cells and can be used to evaluate the potential of a primary antibody …

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Targeting Talk: Product Questions

Could you please tell me if the Somatostatin 14 antibody (Cat. #AB-04) will also pick up the Somatostatin 28 residue? Yes, it will, because they share the sequence of SS14. However, the Somatostatin-28 antibody (Cat. #AB-05) will not see Somatostatin-14. Could you confirm if Anti-Conjugated Caprylic Acid (Cat. #AB-T084) can detect Caprylic acid unconjugated with …

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Targeting Topics 09q2

Noradrenergic Neurons in the Locus Coeruleus Contribute to Neuropathic Pain Brightwell JJ, Taylor BK Neuroscience 160(1):174-185, 2009. Noradrenergic neurons were eliminated with 5 µg intracerebroventricular injections of anti-DBH-SAP (Cat. #IT-03). Mouse IgG-SAP (Cat. #IT-18) was used as a control. Animals lesioned with anti-DBH-SAP displayed a reduction in behavioral signs of several kinds of allodynia. Neuropeptide …

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Cover Article: Deletion of Catecholaminergic Neurons by Anti-DBH-Saporin Disrupts Hypothalamic MAP Kinase and CREB Activation

Contributed by Arshad M. Khan, Kimberly L. Rapp, Todd A. Ponzio, Graciela Sanchez-Watts and Alan G. Watts; Dept. of Biological Sciences and Neuroscience Research Institute, University of Southern California, Los Angeles, CA;  and Laboratory for Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD The brain has evolved adaptive mechanisms for …

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