Human IgG-SAP [IT-49]

a tool for use as control for human IgG-containing immunolesioning agents; non-targeted via pre-immune human IgG antibody conjugated to saporin

SKU: IT-49 Category: Quantity: 25 ug, 100 ug, 250 ug | Host: human | Reactivity: no known specificity | Conjugate: saporin | Usage: control |

Human IgG-SAP serves as a control for the human IgG-containing immunolesioning agents.  Conjugates utilizing human or humanized antibodies will benefit from using Human IgG-SAP as it is manufactured in an identical manner as other targeted conjugates, to give a definitive baseline for comparison.

Human IgG-SAP serves as a control for human IgG-containing immunolesioning agents.

keywords: saporin, control, IgG, human, whole

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Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin.

Kohls MD, Lappi DA (2000) Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin. BioTechniques 28(1):162-165. doi: 10.2144/00281pf01

Summary: Immunotoxins are useful tools for elimination of specific cell populations in vitro and in vivo for research and therapeutic applications. One of the factors limiting the use of immunotoxins is the selection of an appropriate antibody. Advanced Targeting Systems has created a reagent that allows researchers to select antibodies with the desired characteristics before an immuntoxin is made, purified, and assayed. Using a goat anti-murine IgG coupled to the ribosome-inactivating protein saporin, researchers can screen hundreds of antibodies in a time and cost-effective manner.

Related Products: Mab-ZAP (Cat. #IT-04), Rab-ZAP (Cat. #IT-05), Hum-ZAP (Cat. #IT-22), Rat-ZAP (Cat. #IT-26), Anti-M-ZAP (Cat. #IT-30), Goat-ZAP (Cat. #IT-36)

Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184

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Abstract

Saporin is a ribosome-inactivating protein that can cause inhibition of protein synthesis and causes cell death when delivered inside a cell. Development of commercial Saporin results in a technology termed ‘molecular surgery’, with Saporin as the scalpel. Its low toxicity (it has no efficient method of cell entry) and sturdy structure make Saporin a safe and simple molecule for many purposes. The most popular applications use experimental molecules that deliver Saporin via an add-on targeting molecule. These add-ons come in several forms: peptides, protein ligands, antibodies, even DNA fragments that mimic cell-binding ligands. Cells that do not express the targeted cell surface marker will not be affected. This review will highlight some newer efforts and discuss significant and unexpected impacts on science that molecular surgery has yielded over the last almost four decades. There are remarkable changes in fields such as the Neurosciences with models for Alzheimer’s Disease and epilepsy, and game-changing effects in the study of pain and itch. Many other uses are also discussed to record the wide-reaching impact of Saporin in research and drug development.

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Targeting Tools: Control Immunotoxins

Control Conjugates – The Perfect Companion for Targeted Toxins

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Cytotoxicity Assay Protocol for Control Conjugates

Online Calculator: Preparing Control Conjugate Samples

Concentration Calculations: Convert molarity to mg/ml and mg/ml to molarity (PDF worksheet)

Preparing and Interpreting Cytotoxicity Data in vitro


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