FGF-SAP [IT-38, KIT-38]

a tool for eliminating cells that express FGF receptor; targeted via rat FGF-2, or basic fibroblast growth factor, eliminated via saporin

SKU: IT-38 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug | Host: rat | Reactivity: mammalian | Conjugate: saporin | Usage: eliminates cells |

FGF-2, or basic fibroblast growth factor, binds all of the FGF receptors with high affinity. We have used this molecule to produce FGF-SAP, which has a healthy experimental publication record. It has been used to clean primary cultures of fibroblasts. It was important in determining the role of smooth muscle cells in restenosis of damaged vasculature. It was widely used in vivo for the elimination of FGF receptor-expressing cells, including neuronal cell types, cancer cells, and lens epithelial cells. FGF-2 has been well characterized demonstrating binding to all four of the FGF high-affinity tyrosine kinase transmembrane receptors including binding to FGF receptor-5 with lower affinity. FGF-SAP will be useful for the study of systems biology.

FGF-SAP is a chemical conjugate of rat FGF-2, or basic fibroblast growth factor, and the ribosome-inactivating protein, saporin. It specifically eliminates cells expressing the FGF receptor.

FGF-SAP is available individually (Cat. #IT-38) or as a kit (Cat. #KIT-38) which includes FGF-SAP  and Saporin (Cat. #PR-01).

keywords: FGF, FGF2, FGF-2, FGF receptor, basic fibroblast growth factor, saporin, smooth muscle, restenosis, cancer, tyrosine kinase, brain, neuroscience

Reviews

There are no reviews yet.

Be the first to review “FGF-SAP [IT-38, KIT-38]”

View Data Sheet

Roles of the FGF-FGFR signaling system in cancer development and inflammation

Wiedlocha A, Haugsten EM, Zakrzewska M (2021) Roles of the FGF-FGFR signaling system in cancer development and inflammation. Cells 10(9):2231. doi: 10.3390/cells10092231 PMID: 34571880

Objective: To highlight the latest advances in understanding the role of the FGF-FGFR signaling system in the development of neoplastic diseases and in the induction and maintenance of inflammation and its sequelae.

Related Products: FGF-SAP (Cat. #IT-38)


Efficacy and selectivity of FGF2-saporin cytosolically delivered by PCI in cells overexpressing FGFR1

Vikan AK, Kostas M, Haugsten EM, Selbo PK, Wesche J (2021) Efficacy and selectivity of FGF2-saporin cytosolically delivered by PCI in cells overexpressing FGFR1. Cells 10(6):1476. doi: 10.3390/cells10061476

Summary: Fibroblast growth factor receptors (FGFRs) have become an attractive target in cancer research and therapy due to their implication in several cancers. The authors evaluated the efficacy and selectivity of PCI of FGF2-saporin (FGF-SAP) in cells overexpressing FGFR1. The authors conclude that to prevent off-target effects of FGF-based toxins, it will be necessary to circumvent binding to HSPGs, for example by mutating the binding site of FGF2 to HSPGs.

Related Products: FGF-SAP (Cat. #IT-38)

Expression and activities of a recombinant basic fibroblast growth factor-saporin fusion protein

Lappi DA, Ying W, Barthelemy I, Martineau D, Prieto I, Benatti L, Soria M, Baird A (1994) Expression and activities of a recombinant basic fibroblast growth factor-saporin fusion protein. J Biol Chem 269(17):12552-12558. PMID: 8175664

Related Products: FGF-SAP (Cat. #IT-38)


browse all references for this product | back to top

FGF Products:

FGF-SAP (Cat. #IT-38)

Fibroblast Growth Factor-2 Rat Recombinant (Cat. #PR-09)

Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)

Fibroblast Growth Factor Rabbit Polyclonal, rat (Cat. #AB-08)

Targeting Tools: Anti-basic FGF (FGF-2)

Targeting Tools: Targeting FGF Receptors

Cytotoxicity Assay for Targeted Toxins in vitro

Concentration Calculation Explained: Convert molarity to mg/ml and mg/ml to molarity

Preparing and Interpreting Cytotoxicity Data in vitro


browse all protocols and calculators | back to top

Shopping Cart
Scroll to Top