FGF-2, or basic fibroblast growth factor, binds all of the FGF receptors with high affinity. We have used this molecule to produce FGF-SAP, which has a healthy experimental publication record (“FGF” and “saporin” in PubMed: 25 hits). It has been used to clean primary cultures of fibroblasts. It was important in determining the role of smooth muscle cells in restenosis of damaged vasculature. It was widely used in vivo for the elimination of FGF receptor-expressing cells, including neuronal cell types, cancer cells, and lens epithelial cells. FGF-2 has been well characterized demonstrating binding to all four of the FGF high-affinity tyrosine kinase transmembrane receptors including binding to FGF receptor-5 with lower affinity. FGF-SAP will be useful for the study of systems biology.
FGF-SAP is a chemical conjugate of rat FGF-2, or basic fibroblast growth factor, and the ribosome-inactivating protein, saporin. It specifically eliminates cells expressing the FGF receptor.