FGF-2 is a 155 amino acid single-chain polypeptide heparin binding growth factor with mitogenic and angiogenic properties. It is a potent inducer of DNA synthesis in a variety of cell types. FGF-2 is required for the maintenance of human embryonic stem cells in culture, as well as various other stem cell lines. It is also involved in a variety of other cell processes, including cell growth, morphogenesis, tissue repair, tumor growth, and embryonic development.
Rat recombinant FGF-2 (basic fibroblast growth factor) is produced in bacteria and purifed by heparin sepharose affinity chromatography. The purified protein has been sterile filtered. The activity of this protein is tested by proliferation assay on 3T3 cells.
FGF-2 can be used to optimize cell culture conditions for a variety of cell types. It also has uses in bone regeneration and angiogenesis models.
keywords: Fibroblast Growth Factor, FGF, FGF-2, bFGF