FGF-2, or basic fibroblast growth factor, binds all of the FGF receptors with high affinity. We have used this molecule to produce FGF-SAP, which has a healthy experimental publication record. It has been used to clean primary cultures of fibroblasts. It was important in determining the role of smooth muscle cells in restenosis of damaged vasculature. It was widely used in vivo for the elimination of FGF receptor-expressing cells, including neuronal cell types, cancer cells, and lens epithelial cells. FGF-2 has been well characterized demonstrating binding to all four of the FGF high-affinity tyrosine kinase transmembrane receptors including binding to FGF receptor-5 with lower affinity. FGF-SAP will be useful for the study of systems biology.
FGF-SAP is a chemical conjugate of rat FGF-2, or basic fibroblast growth factor, and the ribosome-inactivating protein, saporin. It specifically eliminates cells expressing the FGF receptor.
FGF-SAP is available individually (Cat. #IT-38) or as a kit (Cat. #KIT-38) which includes FGF-SAP and Saporin (Cat. #PR-01).
keywords: FGF, FGF2, FGF-2, FGF receptor, basic fibroblast growth factor, saporin, smooth muscle, restenosis, cancer, tyrosine kinase, brain, neuroscience
Roles of the FGF-FGFR signaling system in cancer development and inflammation
Wiedlocha A, Haugsten EM, Zakrzewska M (2021) Roles of the FGF-FGFR signaling system in cancer development and inflammation. Cells 10(9):2231. doi: 10.3390/cells10092231 PMID: 34571880
Objective: To highlight the latest advances in understanding the role of the FGF-FGFR signaling system in the development of neoplastic diseases and in the induction and maintenance of inflammation and its sequelae.
Related Products: FGF-SAP (Cat. #IT-38)
Efficacy and selectivity of FGF2-saporin cytosolically delivered by PCI in cells overexpressing FGFR1
Vikan AK, Kostas M, Haugsten EM, Selbo PK, Wesche J (2021) Efficacy and selectivity of FGF2-saporin cytosolically delivered by PCI in cells overexpressing FGFR1. Cells 10(6):1476. doi: 10.3390/cells10061476
Summary: Fibroblast growth factor receptors (FGFRs) have become an attractive target in cancer research and therapy due to their implication in several cancers. The authors evaluated the efficacy and selectivity of PCI of FGF2-saporin (FGF-SAP) in cells overexpressing FGFR1. The authors conclude that to prevent off-target effects of FGF-based toxins, it will be necessary to circumvent binding to HSPGs, for example by mutating the binding site of FGF2 to HSPGs.
Related Products: FGF-SAP (Cat. #IT-38)
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