Acetylated LDL-SAP [IT-08, KIT-08]

a tool for eliminating cells that express microglia in primary cell cultures; targeted via acetylated human low density lipoprotein, eliminated via saporin

SKU: IT-08 Category: Host: Human | Reactivity: rat | Conjugate: saporin | Usage: eliminates cells |
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Acetylated-LDL-Saporin (acLDL-SAP) has been used effectively to eliminate microglia from primary cultures of rat hippocampal neurons, while having no deleterious effect on neurons or astroglia. After 24 hours exposure, 3 µg/ml of acLDL-SAP selectively kills more than 90% of microglia cells. acLDL-SAP is excellent for removing contaminating macrophages from primary cultures to determine their role(s) in autoimmune diseases and in degenerative diseases such as Alzheimer’s.

Acetylated LDL-SAP is a chemical conjugate of acetylated human low density lipoprotein and the ribosome-inactivating protein, saporin. It eliminates scavenger receptor-positive cells. This includes the macrophage and its relative, the microglia.

Acetylated LDL-SAP is available individually (Cat. #IT-08) or as a kit (Cat. #KIT-08) which includes Acetylated LDL-SAP and Saporin (Cat. #PR-01).