Acetylated-LDL-Saporin (acLDL-SAP) has been used effectively to eliminate microglia from primary cultures of rat hippocampal neurons, while having no deleterious effect on neurons or astroglia. After 24 hours exposure, 3 µg/ml of acLDL-SAP selectively kills more than 90% of microglia cells. acLDL-SAP is excellent for removing contaminating macrophages from primary cultures to determine their role(s) in autoimmune diseases and in degenerative diseases such as Alzheimer’s.
Acetylated LDL-SAP is a chemical conjugate of acetylated human low density lipoprotein and the ribosome-inactivating protein, saporin. It eliminates scavenger receptor-positive cells. This includes the macrophage and its relative, the microglia.
Acetylated LDL-SAP is available individually (Cat. #IT-08) or as a kit (Cat. #KIT-08) which includes Acetylated LDL-SAP and Saporin (Cat. #PR-01).
keywords: acetylated-LDL, acLDL, Acetylated low density lipoprotein, microglia, macrophage, scavenger receptor, autoimmune disease, saporin, Alzheimer’s disease, brain, neuroscience
Targeted toxins in pain.
Wiley RG, Lappi DA (2003) Targeted toxins in pain. Adv Drug Deliv Rev 55(8):1043-1054. doi: 10.1016/s0169-409x(03)00102-9
Summary: The authors discuss the use of 'molecular neurosurgery' in the study of nociception. Applications using targeted toxins, which include immunotoxins, protein-toxin conjugates, or peptide-toxin conjugates, are illustrated. The authors describe the use of these molecules as research tools, as well as their potential for therapeutics. A helpful table is included that lists neuronal surface markers and class of cells targeted for each targeted toxin. Reagents discussed: CTB-SAP (Cat. #IT-14), IB4-SAP (Cat. #IT-10), OX7-SAP (Cat. #IT-02), 192-Saporin (Cat. #IT-01), ME20.4-SAP (Cat. #IT-15), Anti-DBH-SAP (Cat. #IT-03), Anti-DAT-SAP (Cat. #IT-25), SP-SAP (Cat. #IT-07), Dermorphin-SAP (Cat. #IT-12), Orexin-SAP (Cat. #IT-20), CRF-SAP (Cat. #IT-13), and acetylated LDL-SAP (Cat. #IT-08).
Related Products: CTB-SAP (Cat. #IT-14), IB4-SAP (Cat. #IT-10), OX7-SAP (Cat. #IT-02), 192-IgG-SAP (Cat. #IT-01), ME20.4-SAP (Cat. #IT-15), Anti-DBH-SAP (Cat. #IT-03), Anti-DAT-SAP (Cat. #IT-25), SP-SAP (Cat. #IT-07), Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Orexin-B-SAP (Cat. #IT-20), CRF-SAP (Cat. #IT-13), Acetylated LDL-SAP (Cat. #IT-08)
Study of receptor-mediated neurotoxins released by HIV-1-infected, mononuclear phagocytes found in human brain.
Giulian D, Yu J, Li X, Tom D, Li J, Wendt E, Lin S-N, Schwarcz R, Noonan C (1996) Study of receptor-mediated neurotoxins released by HIV-1-infected, mononuclear phagocytes found in human brain. J Neurosci 16:3139-3153. doi: 10.1523/JNEUROSCI.16-10-03139.1996
Related Products: Acetylated LDL-SAP (Cat. #IT-08)
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