- Home
- Knowledge Base
- Search Results for Stem Cells
Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy
Jiao J, Tian W, Qiu P, Norton EL, Wang MM, Chen YE, Yang B (2018) Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy. J Thorac Cardiovasc Surg 156:515-522. doi: 10.1016/j.jtcvs.2018.02.087 PMID: 29653750
Objective: To develop an in vitro model with human-induced pluripotent stem cells (iPSCs) to evaluate the role of NOTCH1 in smooth muscle and endothelial cell differentiation.
Summary: NOTCH1 is critical in SMC and EC differentiation of iPSCs through neural crest stem cells and cardiovascular progenitor cells, respectively. NOTCH1gene mutations may potentially contribute to the development of thoracic aortic aneurysms by affecting SMC differentiation in some patients with bicuspid aortic valve-related aortopathy.
Usage: Immunofluorescence staining and flow cytometry was performed.
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Low osteogenic yield in human pluripotent stem cells associates with differential neural crest promoter methylation
Sparks NRL, Martinez IKC, Soto CH, Zur Nieden NA-O (2018) Low osteogenic yield in human pluripotent stem cells associates with differential neural crest promoter methylation. Stem Cells 36:349-362. doi: 10.1002/stem.2746 PMID: 29193426
Objective: To compare the osteogenic potential of two human induced pluripotent stem cell lines (RIV9 and RIV4) to human H9 embryonic stem cells.
Summary: The study demonstrates that different hPSC lines, including individual hiPSC clones, could have inherently different abilities to produce functional osteoblasts potentially based on the methylation marks placed on promotor regions important for lineage decisions
Usage: immunohistochemistry
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Regenerative effects of human embryonic stem cell-derived neural crest cells for treatment of peripheral nerve injury
Jones I, Novikova LN, Novikov LN, Renardy M, Ullrich A, Wiberg M, Carlsson L, Kingham PJ (2018) Regenerative effects of human embryonic stem cell-derived neural crest cells for treatment of peripheral nerve injury. J Tissue Eng Regen Med 12(4):e2099-e2109. doi: 10.1002/term.2642 PMID: 29327452
Objective: To determine if differentiated neural crest cells are promising supporting cell candidates to aid in peripheral nerve repair.
Summary: In this study, neural crest cells were differentiated from human embryonic stem cells. NGFR (mouse monoclonal, 1:100) Immunocytochemistry. The specificity of NGFR antibody was validated by immunocytochemical staining of both hESCs- and MACS-enriched cells.
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Extracellular matrix from periodontal ligament cells could induce the differentiation of induced pluripotent stem cells to periodontal ligament stem cell-like cells
Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H (2017) Extracellular matrix from periodontal ligament cells could induce the differentiation of induced pluripotent stem cells to periodontal ligament stem cell-like cells. Stem Cells Dev 27:100-111. doi: 10.1089/scd.2017.0077 PMID: 29160151
Usage: Immunofluorescence staining (1:200 dilution)
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Neurotrophically induced mesenchymal progenitor cells derived from induced pluripotent stem cells enhance neuritogenesis via neurotrophin and cytokine production
Brick RM, Sun AX, Tuan RSA-Ohoo (2018) Neurotrophically induced mesenchymal progenitor cells derived from induced pluripotent stem cells enhance neuritogenesis via neurotrophin and cytokine production. Stem Cells Transl Med 7:45-58. doi: 10.1002/sctm.17-0108 PMID: 29215199
Objective: To examine the potential utility of an alternative, mesenchymal-like cell (MSCs) source, derived from induced pluripotent stem cells, termed induced mesenchymal progenitor cells (MiMPCs) to produce bioactive factors and support nerve regeneration.
Summary: The findings suggest MiMPCs as a renewable, candidate source of therapeutic cells and a potential alternative to MSCs for peripheral nerve repair, in view of their ability to promote nerve growth by producing many of the same growth factors and cytokines as Schwann cells and signaling through critical neurotrophic pathways.
Usage: Immunofluorescence
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Directed differentiation of periocular mesenchyme from human embryonic stem cells
Lovatt M, Yam GH-F, Peh GS, Colman A, Dunn NR, Mehta JS (2018) Directed differentiation of periocular mesenchyme from human embryonic stem cells. Differentiation 99:62-69. doi: 10.1016/j.diff.2017.11.003 PMID: 29239730
Objective: Pluripotent stem cells are attractive sources of cells for regenerative medicine, because large numbers of therapeutically useful cells can be generated. However, a detailed understanding of how to differentiate clinically relevant cell types from stem cells is fundamentally required.
Summary: Identification of cells resembling periocular mesenchyme (POM) cells in the adult cornea, located in a niche between the trabecular meshwork and peripheral endothelium. The generation and expansion of POM is an important step in the generation of a number of cells types that could prove to be clinically useful for a number of diseases of the cornea.
Usage: 1:200 for flow cytometry and immunofluorescence.
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Preclinical modeling highlights the therapeutic potential of hematopoietic stem cell gene editing for correction of SCID-X1.
Schiroli G, Ferrari S, Conway A, Jacob A, Capo V, Albano L, Plati T, Castiello M, Sanvito F, Gennery A, Bovolenta C, Palchaudhuri R, Scadden D, Holmes M, Villa A, Sitia G, Lombardo A, Genovese P, Naldini L (2017) Preclinical modeling highlights the therapeutic potential of hematopoietic stem cell gene editing for correction of SCID-X1. Sci Transl Med 9(411):eaan0820. doi: 10.1126/scitranslmed.aan0820
Objective: To study potential approaches to gene therapy in mouse models of severe combined immunodeficiency.
Summary: The threshold of IL2RG gene editing can be reached for safe and efficient correction of SCID-X1 established in a preclinical model in human long-term repopulating HSPCs.
Usage: Biotinylated Anti-CD45 was mixed equimolar to Streptavidin-ZAP and administered as a single dose which caused substantial depletion (~70%) of the HSPC compartments and milder depletion of the more mature cell populations.
Related Products: Streptavidin-ZAP (Cat. #IT-27), Anti-CD45.2-SAP (Cat. #IT-91)
Therapeutic Potential of Hematopoietic Stem Cell Gene Editing
Directed differentiation of human bone marrow stromal cells to fate-committed schwann cells
Cai S, Tsui YP, Tam KW, Shea GK, Chang RS, Ao Q, Shum DK, Chan YS (2017) Directed differentiation of human bone marrow stromal cells to fate-committed schwann cells. Stem Cell Reports 9(4):1097-1108. doi: 10.1016/j.stemcr.2017.08.004 PMID: 28890164
Objective: To create a protocol for in vitro derivation of fate-committed Schwann cells (SCs) from human bone marrow stromal cells (BMSCs).
Summary: This study demonstrates that the human bone marrow harbors neuro-ectodermal progenitors that can be enriched, expanded, and directed to differentiate into functionally mature, fate-committed SCs.
Usage: IF (1:500) and WB (1:500)
Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
LNGFR targets the Wnt/β-catenin pathway and promotes the osteogenic differentiation in rat ectomesenchymal stem cells
Li G, Liu J, Wang Y, Yang K, Zhao M, Xiao Y, Wen X, Liu L (2017) LNGFR targets the Wnt/β-catenin pathway and promotes the osteogenic differentiation in rat ectomesenchymal stem cells. Sci Rep 7(1):11021. doi: 10.1038/s41598-017-11555-9 PMID: 28887537
Objective: To demonstrate that nuclear β-catenin was upregulated during ectomesenchymal stem cells (EMSC) osteogenic differentiation.
Summary: The data reveal that the low affinity nerve growth factor receptor (LNGFR) targets the Wnt/β-catenin pathway and positively regulates EMSC osteogenic differentiation, suggesting that Wnt/β-catenin pathway may be involved in the development of teeth and that the targeting Wnt/β-catenin pathway may have great potential for applications in dental tissue engineering regeneration.
Usage: Western blot
Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Induced pluripotent stem cell-derived mesenchymal progenitor cells: Promotion of neuritogenesis and axon elongation through neurotrophin and cytokine production
Brick, R (2017) Induced pluripotent stem cell-derived mesenchymal progenitor cells: Promotion of neuritogenesis and axon elongation through neurotrophin and cytokine production. University of Pittsburgh Thesis.
Objective: To test if mesenchymal progenitor cells (MiMPCs), originally derived from mesenchymal stem cells (MSCs), have the same ability to support neuronal regeneration as the parent MSC.
Summary: Results show that MiMPCs can secrete neurotrophic factors after neuroinductive treatment and can produce factors to improve neurite outgrowth in a chick embryonic dorsal root ganglion (DRG) model. These findings suggest that neurotrophically induced-MiMPCs (NI-MiMPCs) may be considered a suitable substitute cell type to support nerve growth.
Usage: immunofluorescence
Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)