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2021 Targeting Trends Review
Integrin α10-antibodies reduce glioblastoma tumor growth and cell migration
Masoumi KC, Huang X, Sime W, Mirkov A, Munksgaard Thorén M, Massoumi R, Lundgren-Åkerlund E (2021) Integrin α10-antibodies reduce glioblastoma tumor growth and cell migration. Cancers (Basel) 13(5):1184. doi: 10.3390/cancers13051184
Summary: The authors investigated the treatment effect of two antibodies that have been developed to target the protein integrin 10, which is present on the surface of Glioblastoma (GB) cells. Function-blocking integrin alpha10, beta1-antibodies inhibit GB tumor growth as well as the migration of GB cells. This further validates integrin alpha10, beta1 as a promising target in GB and suggests a novel therapeutic strategy for the treatment of GB and other high-grade gliomas.
Usage: Infusions of anti-integrin α10β1-SAP or Anti-ctrl-SAP were made icv (1 µg/2 L per infusion).
Related Products: Custom Conjugates
Activated factor X targeted stored in platelets as an effective gene therapy strategy for both hemophilia A and B
Wang D, Shao X, Wang Q, Pan X, Dai Y, Yao S, Yin T, Wang Z, Zhu J, Xi X, Chen Z, Chen S, Zhang G (2021) Activated factor X targeted stored in platelets as an effective gene therapy strategy for both hemophilia A and B. Clin Transl Med 11(3):e375. doi: 10.1002/ctm2.375
Summary: Treatment of hemophiliacs with inhibitors remains challenging, and new treatments are in urgent need. Coagulation factor X plays a critical role in downstream blood coagulation cascade, which could serve as a bypassing agent for hemophilia therapy. Target expression of the FXa precursor to platelets can generate a storage pool of FXa in platelet α-granules, the platelet-stored FXa is effective in treating HA and HB with inhibitors, suggesting that this could be a novel choice for hemophilia patients with inhibitors.
Usage: A single dose of CD45.2-SAP (biotinylated Anti-CD45 mixed with Streptavidin-ZAP) enabled efficient engraftment of donor cells (> 90%) and full correction of sickle-cell anemia. (3 mg/kg iv; Palchaudhuri et al.).
Related Products: Streptavidin-ZAP (Cat. #IT-27)
Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection
Lind LA, Lever TE, Nichols NL (2021) Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection. Muscle Nerve 63(3):413-420. doi: 10.1002/mus.27131
Objective: To evaluate tongue morphology and ultrastructural changes in hypoglossal neurons and nerve fibers in an inducible rat model of dysphagia.
Summary: Preliminary results indicate this model may have translational application to a variety of neurodegenerative diseases resulting in tongue dysfunction and associated dysphagia.
Usage: Rats assigned to the CTB-SAP group (n = 10) received 25 μg CTB-SAP to produce hypoglossal motor neuron death.
Related Products: CTB-SAP (Cat. #IT-14)
Intact vagal gut-brain signalling prevents hyperphagia and excessive weight gain in response to high-fat high-sugar diet.
McDougle M, Quinn D, Diepenbroek C, Singh A, de la Serre C, de Lartigue G (2021) Intact vagal gut-brain signalling prevents hyperphagia and excessive weight gain in response to high-fat high-sugar diet. Acta Physiol (Oxf) 231(3):e13530. doi: 10.1111/apha.13530
Objective: To assess the function of the vagus nerve lack specificity.
Summary: Intact sensory vagal neurons prevent hyperphagia and exacerbation of weight gain in response to a HFHS diet by promoting lipid-mediated satiation.
Usage: Rat nodose ganglia were injected bilaterally with either CCK-SAP or unconjugated saporin as a control.
Related Products: CCK-SAP (Cat. #IT-31)
Coiled coil-based therapeutics and drug delivery systems
Utterström J, Naeimipour S, Selegård R, Aili D (2021) Coiled coil-based therapeutics and drug delivery systems. Adv Drug Deliv Rev 170:26-43. doi: 10.1016/j.addr.2020.12.012
Related Products: Saporin (Cat. #PR-01)
Saporin, a polynucleotide-adenosine nucleosidase, may be an efficacious therapeutic agent for SARS-CoV-2 infection
Arslan I, Akgul H, Kara M (2021) Saporin, a polynucleotide-adenosine nucleosidase, may be an efficacious therapeutic agent for SARS-CoV-2 infection. SLAS Discov 26(3):330-335. doi: 10.1177/2472555220970911
Related Products: Saporin (Cat. #PR-01)
Minocycline in neurodegenerative and psychiatric diseases: An update.
Romero-Miguel D, Lamanna-Rama N, Casquero-Veiga M, Gómez-Rangel V, Desco M, Soto-Montenegro ML (2021) Minocycline in neurodegenerative and psychiatric diseases: An update. Eur J Neurol 28(3):1056-1081. doi: 10.1111/ene.14642
Summary: Review includes the mouse animal model of neurodegenerative disease using mu p75-SAP. Biological Effects: Attenuation of cholinergic neurons loss, glial activation and transcription of pro-inflammatory mediators.
Usage: 45 mg/Kg, i.p.
Related Products: mu p75-SAP (Cat. #IT-16)
Central opioid receptors mediate morphine-induced itch and chronic itch via disinhibition
Wang Z, Jiang C, Yao H, Chen O, Rahman S, Gu Y, Zhao J, Huh Y, Ji RR (2021) Central opioid receptors mediate morphine-induced itch and chronic itch via disinhibition. Brain 144(2):665-681. doi: 10.1093/brain/awaa430
Summary: Itch is a common side effect of opioids, particularly as a result of epidural or intrathecal administration. Notably, morphine-elicited itch was suppressed by intrathecal administration of NPY and abolished by spinal ablation of GRPR+ neurons with intrathecal injection of Bombesin-SAP.
Usage: For ablation of GRPR+ neurons, mice were given an intrathecal injection of 400 ng Bombesin-SAP or Blank-SAP (control) 10 days before behavioral testing.
Related Products: Bombesin-SAP (Cat. #IT-40), Blank-SAP (Cat. #IT-21)
The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.
Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, Huang H, Fu W, Liang J, Wu W, Li B, Yao H, Hu H, Song E (2021) The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. Nat Commun 12(1):1341. doi: 10.1038/s41467-021-21535-3 PMID: 33637716
Objective: To investigate the contributions of HIF-1 in regulating glycolysis of cancer cells under hypoxia
Summary: HIF Antisense LncRNA (HIFAL) has a critical regulatory role in HIF-1-driven transactivation and glycolysis, identifying HIFAL as a therapeutic target of cancer treatment.
Usage: Western blot (1:500)
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Fluoxetine and ketamine reverse the depressive but not anxiety behavior induced by lesion of cholinergic neurons in the horizontal limb of the diagonal band of broca in male rat
Chen L, Ke Y, Ma H, Gao L, Zhou Y, Zhu H, Liu H, Zhang F, Zhou W (2021) Fluoxetine and ketamine reverse the depressive but not anxiety behavior induced by lesion of cholinergic neurons in the horizontal limb of the diagonal band of broca in male rat. Front Behav Neurosci 15:602708. doi: 10.3389/fnbeh.2021.602708
Summary: A lesion of horizontal limb of the diagonal band of Broca (HDB) cholinergic neurons and followed hippocampus damage may be involved in the pathogenesis of depression.
Usage: Injections of 192-IgG-SAP were made bilaterally into the HDB in a volume of 0.5 µL per side with a concentration of 0.5 µg/µL.
Related Products: 192-IgG-SAP (Cat. #IT-01)