2001 Targeting Trends Review

Grindstaff RJ, Grindstaff RR, Cunningham JT (2000) Baroreceptor sensitivity of rat supraoptic vasopressin neurons involves noncholinergic neurons in the DBB. Am J Physiol Reul Integr Comp Physiol 279:R1934-R1943. doi: 10.1152/ajpregu.2000.279.5.R1934

Summary: Baroreceptors are one component of the system that buffers acute changes in blood pressure. Part of this control stems from the baroreceptor ability to regulate vasopressin release from the neurohypophysis. Using 192-Saporin (Cat. #IT-01) to specifically eliminate cholinergic neurons in the diagonal band of Broca, Grindstaff et al. demonstrated that these neurons are not utilized in the pathway that relays baroreceptor information to the brain.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Gu Z, Wortwein G, Yu J, Perez-Polo JR (2000) Model for aging in the basal forebrain cholinergic system. Antiox Redox Signal 2(3):437-447. doi: 10.1089/15230860050192215

Summary: A wide range of evidence indicates that cholinergic neurons play a role in memory and learning. Loss of these neurons is seen both in aged subjects and Alzheimer’s Disease patients. The authors discuss the use of 192-Saporin (Cat. #IT-01) to model this phenomenon. Many lesioning methods have been developed, including fimbria-fornix transections, mechanical lesions with radiofrequency or electrolysis, and intracerebral injections of excitotoxins. Information obtained through these methods suffers because non-cholinergic neurons are depleted as well as the desired cholinergic neurons. 192-Saporin provides a solution by specifically targeting and eliminating cholinergic neurons expressing p75 in the basal forebrain, closely mimicking a key component of aging.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Silveira DC, Holmes GL, Schachter SC, Geula C, Schomer DL (2000) Increased susceptibility to generalized seizures after immunolesions of the basal forebrain cholinergic neurons in rats. Brain Res 878:223-227. doi: 10.1016/s0006-8993(00)02703-7

Usage: 192-SAP (Cat. #IT-01), 4 µg intracerebroventricular injection

Related Products: 192-IgG-SAP (Cat. #IT-01)

Taylor BK, Roderick RE, Basbaum AI (2000) Brainstem noradrenergic control of nociception is abnormal in the spontaneously hypertensive rat. Neurosci Lett 291:139-142. doi: 10.1016/s0304-3940(00)01389-6

Usage: anti-DBH-SAP (Cat. #IT-03), 5 µg

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Wiley RG (2000) Neuropeptide-toxin conjugates in pain research and treatment. (Review). Reg Anesth Pain Med 25(5):546-548. doi: 10.1053/rapm.2000.8457

Summary: Several lines of evidence indicate dorsal horn neurons that respond to substance P (SP) play a role in nociception. Wiley discusses the attributes of SP-SAP (Cat. #IT-07), a targeted toxin that eliminates cells expressing the neurokinin-1 receptor. Animals treated with this material using a lumbar intrathecal injection show a decrease in both hyperalgesia and allodynia in several pain models. The success of SP-SAP indicates that other neuropeptides, hormones, and growth factors would be useful as targeted toxins.

Related Products: SP-SAP (Cat. #IT-07)

Bannerman P, Nichols W, Puhalla S, Oliver T, Berman M, Pleasure D (2000) Early migratory rat neural crest cells express functional gap junctions: Evidence that neural crest cell survival requires gap junction function. J Neurosci Res 61:605-615. doi: 10.1002/1097-4547(20000915)61:6<605::AID-JNR4>3.0.CO;2-U PMID: 10972957

Summary: Gap junctions are vital for intercellular communication, especially during development. Neural crest cells develop into several types of neural cells, often migrating as a mass of cells to their final destinations. Bannerman et al. use the anti-p75 antibody (Cat. #AB-N01) to confirm the presence of p75 in neural crest cells. The authors examine how crucial survival signals are communicated during migration and demonstrate that interfering with gap junction formation causes death of neural crest cells.

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Hunt SP (2000) Pain control: breaking the circuit. Trends Pharmacol Sci 21:284-287. doi: 10.1016/s0165-6147(00)01496-6

Summary: Review and analysis of the value of SP-SAP in research and as a therapeutic.

Related Products: SP-SAP (Cat. #IT-07)

Herron P, Schweitzer JB (2000) Effects of cholinergic depletion on neural activity in different laminae of the rat barrel cortex. Brain Res 872:71-76. doi: 10.1016/s0006-8993(00)02454-9

Summary: 192-SAP (Cat. #IT-01) 8.0 µg/300 g body weight, nucleus basalis of Meynert

Related Products: 192-IgG-SAP (Cat. #IT-01)

Tremere LA, Pinaud R, Grosche J, Hartig W, Rasmusson DD (2000) Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species. NeuroReport 11(10):2177-2183. doi: 10.1097/00001756-200007140-00023 PMID: 10923666

Summary: 192-SAP (Cat. #IT-01) is a highly successful reagent for eliminating cholinergic neurons in rats. Because the targeting antibody only recognizes rat p75, it is unable to be used in other species. Tremere et al. have stained basal forebrain sections with ME20.4, a monoclonal antibody to human p75 (Cat. #AB-N07) and found excellent cross-reactivity in dog, raccoon, cat, pig and rabbit. The authors state that an ME20.4-saporin conjugate could be used to produce cholinergic basal forebrain lesions in several species.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07), 192-IgG-SAP (Cat. #IT-01)

Wirth S, Lehmann O, Bertrans F, Lazarus C, Jeltsch H, Cassel JC (2000) Preserved olfactory short-term memory after combined cholinergic and serotonergic lesions using 192 IgG-Saporin and 5,7-dihydroxytryptamine in rats [published erratum appears in Neuroreport 2000 Mar 20;11(4):inside back cover]. Neuroreport 11:347-350. doi: 10.1097/00001756-200002070-00025

Usage: 192-SAP (Cat. #IT-01) 2 µg, intracerebroventricular

Related Products: 192-IgG-SAP (Cat. #IT-01)