targeted-toxins

Taxini CL, Moreira TS, Takakura AC, Bicego KC, Gargaglioni LH, Zoccal DB (2017) Role of A5 noradrenergic neurons in the chemoreflex control of respiratory and sympathetic activities in unanesthetized conditions. Neuroscience 354:146-157.. doi: 10.1016/j.neuroscience.2017.04.033

Summary: The authors utilize Anti-DBH-SAP (Cat. #IT-03) to investigate the involvement of the A5 noradrenergic neurons to the basal and chemoreflex control of the sympathetic and respiratory activities in unanesthetized conditions.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Taylor BK, Westlund KN (2017) The noradrenergic locus coeruleus as a chronic pain generator. J Neurosci Res 95(6):1336-1346. doi: 10.1002/jnr.23956 PMID: 27685982

Objective: To evaluate whether noradrenergic neurons of the locus coeruleus contribute to the generation and maintenance of chronic neuropathic pain rather than serving solely a pain inhibitory role.

Summary: This review synthesizes evidence showing that noradrenergic signaling from the locus coeruleus can facilitate neuropathic pain following nerve injury, challenging the traditional view of the LC as purely analgesic. Collectively, lesion, pharmacological, and circuit-level studies support a time dependent shift from pain inhibition to pain facilitation mediated by noradrenergic pathways.

Usage: This review summarizes multiple studies that utilized Anti-DBH-SAP (IT-03) to selectively ablate central noradrenergic neurons via intracerebroventricular or region-specific injections, demonstrating reduced mechanical and cold hypersensitivity in rodent models of neuropathic pain.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Lee S, Cho W, Lee Y, Han J (2018) Impact of chronic stress on the spatial learning and GR-PKAc-NF-κB signaling in the hippocampus and cortex in rats following cholinergic depletion. Mol Neurobiol 55:3976-3989. doi: 10.1007/s12035-017-0620-5

Objective: Examine the effects of chronic stress on cognitive status and GR-PKAc-NF-κB signaling in rats with a loss of cholinergic input to the hippocampus and cortex.

Summary: The activation of NF-κB induced by cholinergic depletion appears to be aggravated by chronic stress, and this might explain the increased susceptibility of patients with Alzheimer’s disease to stress since activation of NF-κB is associated with stress.

Usage: Male Sprague-Dawley rats received injections of 192 IgG-SAP dissolved in sterile 0.01 M PBS) at a concentration of 0.25 μg/μl.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Havelin J, Imbert I, Sukhtankar D, Remeniuk B, Pelletier I, Gentry J, Okun A, Tiutan T, Porreca F, King T (2017) Mediation of movement-induced breakthrough cancer pain by IB4-binding nociceptors in rats. J Neurosci 37:5111-5122.. doi: 10.1523/JNEUROSCI.1212-16.2017

Objective: To define a novel preclinical measure of movement-induced breakthrough pain (BTP) that is observed in the presence of morphine controlling ongoing pain.

Summary: Novel compounds targeting IB4-binding nociceptors may improve pain management for cancer pain patients and other patient populations suffering from BTP that is inadequately treated by currently available medications.

Usage: To determine the effect of eliminating input from IB4-binding fibers, separate groups of rats received spinal administration of IB4-SAP or the control, Blank-SAP (3.2 mcg/20 mcl saline) followed by a 10 mcl flush of saline. Movement of an air bubble placed between drug solution and saline was used to monitor progress of the injection.

Related Products: IB4-SAP (Cat. #IT-10), Blank-SAP (Cat. #IT-21)

Craig TA, Brown SM, Nichols NL (2017) Adenosine 2A (A2A) receptor expression in rats with motor neuron death from intrapleural CTB-saporin injections. FASEB J 31(S1):873.14. Experimental Biology 2017 Meeting Abstracts. doi: 10.1096/fasebj.31.1_supplement.873.14

Related Products: CTB-SAP (Cat. #IT-14)

Nichols NL, Tanner M (2017) Phrenic long-term facilitation (pLTF) following acute intermittent hypoxia (AIH) is TrkB and PI3K/Akt dependent in rats with motor neuron death from intrapleural CTB-saporin injections. FASEB J 31(S1):1053.13. Experimental Biology 2017 Meeting Abstracts. doi: 10.1096/fasebj.31.1_supplement.1053.13

Related Products: CTB-SAP (Cat. #IT-14)

Jenrette T, Logue J, Horner KA (2017) Striatal patch compartment lesions reduce habitual behaviors. FASEB J 31(S1):1059.6. Experimental Biology 2017 Meeting Abstracts. doi: 10.1096/fasebj.31.1_supplement.1059.6

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)

Erdos B, Cruickshank NC, Einwag ZD, Schaich CL, Wellman TL (2017) Increased BDNF expression in the paraventricular nucleus of the hypothalamus (PVN) abolishes hypotensive actions of nucleus of the solitary tract (NTS) catecholaminergic neurons. FASEB J 31(S1):866.7. Experimental Biology 2017 Meeting Abstracts. doi: fasebj.31.1_supplement.866.7

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Gulino R, Parenti R, Gulisano M (2017) Sonic hedgehog and TDP-43 participate in the spontaneous locomotor recovery in a mouse model of spinal motoneuron disease. J Funct Morphol Kinesiol 2:11.. doi: 10.3390/jfmk2020011

Summary: The authors used a mouse model of neurotoxic motoneuron depletion to study the role of Sonic hedgehog and TDP-43 in the compensatory changes occurring after the lesion. The injection of cholera toxin-B saporin (CTB-SAP; Cat. #IT-14) into the gastrocnemius muscle caused a partial motoneuron death accompanied by an impairment of locomotion.  Motor activity was significantly restored as soon as one month later.

Related Products: CTB-SAP (Cat. #IT-14)

Barry DM, Munanairi A, Chen Z-F (2018) Spinal mechanisms of itch transmission. Neurosci Bull 34:156-164. doi: 10.1007/s12264-017-0125-2

Related Products: Bombesin-SAP (Cat. #IT-40)