sfn2009

Matchynski JJ, Lowrance S, Rossignol J, Puckett N, Derkorver N, Radwan J, Trainor K, Sandstrom M, Dunbar G (2009) Intracerebroventricular injections of mu-P-75 saporin can produce memory deficits without impairing motor deficits in a mouse model of Alzheimer’s disease. Neuroscience 2009 Abstracts 528.1/H34. Society for Neuroscience, Chicago, IL.

Summary: Intracerebroventricular injections of mu-P-75 saporin (Advanced Targeting Systems, San Diego, CA) effectively and efficiently destroys cholinergic neurons and creates memory deficits in mice, mimicking some of the key symptoms of Alzheimer’s disease. Early attempts to use mu-P-75 saporin in mice required a relatively high mean effective dose (ED50) of 3.6 µg in order to create behavioral deficits (Berger-Sweeney et al., 2001, The Journal of Neuroscience, 21: 8164-8173; Hunter et al, 2004, European Journal of Neuroscience, 19: 3305-3316). Recent advances in producing the saporin have lowered the ED50 to doses to 0.4 µg, although the resulting memory deficits are transient, and doses above 0.8 µg can cause motor deficits (Moreau et al., 2008, Hippocampus, 18: 610-622). In an effort to elucidate the behavioral effects of a higher (0.8 µg) dose, we gave bilateral intracerbroventricular injections of mu-P-75 saporin (n=6) or sterile phosphate buffered saline (n=3) into C57/BL6 mice and assessed their cognitive abilities on both a Morris water maze (MWM) and an object-recognition task, while monitoring their motor abilities using a rotarod task. Mice receiving the mu-P-75 saporin performed significantly worse than sham animals on an object recognition task and tended to have longer latencies and swim paths during the seven days of MWM testing. Importantly, no between-group differences were observed for latency to fall on the rotarod task. Collectively, these results suggest that the 0.8 µg dose of saporin is both safe and effective for mimicking AD-like memory deficits, without causing significant motor deficits.

Related Products: mu p75-SAP (Cat. #IT-16)

Hernandez MA, Pineda JB, Del Valle-Mondragón L, Alcaraz-Zubeldia M, Ríos C, Pérez-Severiano F (2009) Evaluation of the effect of molsidomine on nitregic system in an experimental model of cognitive impairment. Neuroscience 2009 Abstracts 529.24/J10. Society for Neuroscience, Chicago, IL.

Summary: The relationship between nitric oxide (NO) and cholinergic system in brain has been evidenced by using inhibitors of the nitric oxide synthase (NOS) that blocked cognition, while NO donors can facilitate it. Nevertheless, the participation of NO in the recovery of cholinergic deficit due to the administration of a selective cholinergic immunotoxin, 192 IgG saporin (SAP) has not been studied. The aim in this work was to evaluate the modulation of the nitrergic system after the damage induced by SAP and to measure the response to the administration of a NO donor, molsidomine (MOL). We used adult male Wistar rats allocated into either one of 4 groups: 1) vehicle PBS, 0.1M pH 7.4, 2) intraseptal administration of SAP (0.22 µg), 3) MOL ip 4 mg/kg, 4) SAP+MOL. Striatum, prefrontal cortex and hippocampus were dissected out at different times after treatment and quantification of nitrites, NOS activity and expression were performed. Our results show that SAP induces a reduction on the constitutive NOS activity in prefrontal cortex and striatum (54%, 64% respectively compared with control p<0.05); while hippocampal cNOS tended to decrease. MOL alone improved NOS activity in those regions. Neuronal and endothelial NOS expression (nNOS, eNOS) in the same regions did not change significantly. When the nitrites levels were analyzed, changes were region-specific. We conclude that administration of the NO donor promotes the recovery of cNOS activity in the model of cholinergic denervation associated to 192 IgG SAP. Further cognitive studies are being carried out in order to demonstrate the cholinergic recovery by MOL.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Thomsen MS, Hay-Schmidt A, Hansen HH, Mikkelsen JD (2009) Poster: Distinct neural pathways mediate alpha7 nicotinic acetylcholine receptor-dependent activation of the forebrain. Neuroscience 2009 Abstracts 646.2/V14. Society for Neuroscience, Chicago, IL.

Summary: α7 nicotinic acetylcholine receptor (nAChR) agonists are novel drugs candidates for the treatment of cognitive deficits in schizophrenia, which have shown pro-cognitive effects in clinical trials. Selective α7 nAChR agonists, such as SSR180711, activate neurons in the medial prefrontal cortex (mPFC) and shell of the nucleus accumbens (ACCshell) in rats, regions which are important for cognitive function. However, the neural substrates involved in these effects remain elusive. Using retrograde tracing from the mPFC with Cholera Toxin B and immunoreactivity of the immediate-early gene c-Fos, a marker of neuronal activation, we identify the horizontal limb of the diagonal band of broca (HDB) in the basal forebrain as an important site of α7 nAChR activation. Approximately 26% of the cortically projecting neurons in the HDB are activated by acute administration of SSR180711 (10 mg/kg), and the neurons activated by SSR180711 in the HDB are cholinergic. Selective depletion of these cholinergic neurons with 192 IgG-Saporin abolishes the SSR180711-induced activation of the mPFC, but not the ACCshell, demonstrating their critical importance for α7 nAChR-dependent activation of the mPFC. Contrarily, selective depletion of dopaminergic neurons in the ventral tegmental area (VTA) with 6-OHDA abolishes the SSR180711-induced activation of the ACCshell, but not the mPFC or HDB. These results indicate that two distinct neural pathways are activated by SSR180711, involving HDB-to-mPFC and VTA-to-ACCshell projections, respectively. The basal forebrain and mPFC are important for attentional function, and may subserve the pro-cognitive effects of α7 nAChR agonists, whereas activation of the ACCshell is implicated in beneficial effects on the positive symptoms of schizophrenia.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Kline IV RH, Wiley RG (2009) Spinal µ-opiate receptor (MOR)-expressing dorsal horn neurons: Role in modulating pain and opiate analgesia. Neuroscience 2009 Abstracts 560.13/CC72. Society for Neuroscience, Chicago, IL.

Summary: Selective destruction of MOR-expressing interneurons in lamina II of the dorsal horn of the spinal cord increases reflex nocifensive responses to formalin and decreases the anti-nociceptive effects of morphine on the hotplate and in the formalin test. The interpretation of these studies is limited because reflex-based assays may not accurately reflect the cerebral component of nociception. Therefore, we sought to determine the effects of selectively destroying MOR-expressing dorsal horn neurons on baseline operant responses to aversive thermal and mechanical stimuli in a shuttle box task and effects of systemic morphine and naloxone in the same task. The preference apparatus consisted of a 15 X 15 X 30 cm smoked Plexiglas vented chamber placed upon two adjoining temperature-controlled smooth aluminum floor plates (thermal preference task) or one smooth temperature-controlled floor plate adjoined to a room temperature surface covered with 40 grit sandpaper (mechanical preference task). For both preference tasks, response functions were obtained by pairing a 44°C plate or the sandpaper surface with either 11°, 16°, 25°, 38° or 46°C. Rats were intrathecally injected over the lumbar cord with either 625ng of derm-sap (n=7) or blank-sap (n=6) followed by daily thermal or mechanical preference testing on a randomized schedule. Derm-sap treated rats showed enhanced avoidance of aversive thermal stimuli and the aversive mechanical stimulus. Morphine and naloxone significantly altered responses of control rats (blank-sap), but not derm-sap rats, in both thermal and mechanical preference tasks. We interpret these results as showing that the derm-sap lesion produces hyperalgesia/allodynia, impairs the anti-nociceptive and analgesic effects of morphine and therefore indicating that postsynaptic dorsal horn MOR-expressing neurons play a key role in modulating nociception, pain and opiate analgesia. Dysfunction of these neurons may also play a role in pathological pain states.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)

Tai S, Leung L (2009) Facilitation of dentate gyrus population spike may involve septohippocampal GABAergic input. Neuroscience 2009 Abstracts 566.18/EE33. Society for Neuroscience, Chicago, IL.

Summary: The medial septum (MS) is known to modulate the neural circuitry in the hippocampus. It have been demonstrated that, in anaesthetized rats, MS stimulation prior to perforant path stimulation facilitated the population spike in the dentate gyrus and such facilitation was unaffected by muscarinic and nicotinic cholinergic antagonists, suggesting that this facilitation may be mediated by septohippocampal GABAergic neurons. To study the effects of septohippocampal neurons in modulating neural circuitry in the dentate gyrus, selective lesion of septohippocampal GABAergic cells was made by infusion of orexin-saporin in the MS. Evoked field potentials were recorded in the dentate gyrus following stimulation of the medial perforant path in urethane-anesthetized rats using multichannel silicon probes and analyzed as current source density. High-frequency stimulation of the pontis oralis (PnO) activated a hippocampal theta rhythm. Theta peak power was attenuated in lesion rats as compared to control (sham lesion) rats. PnO stimulation resulted in a larger average increase in dentate population spike (pSpike) in control rats compared to lesion rats (26.4 ± 8.8 % vs 5.1 ± 8.7 %) but the difference was not significant (P = 0.1082; control n = 9, lesion n = 8). The number of choline acetyltransferase-immunopositive (cholinergic) cells in the MS was not significantly different (P = 0.88, unpaired t-test) between lesion and control rats, while a significant decrease (about 50%) of the number of parvalbumin-immunopositive GABAergic cells in the MS was observed in lesion as compared to control rats (P<0.05, unpaired t-test). The effect of PnO stimulation on the paired-pulse pSpike response versus interpulse interval (30 - 400 ms) was not different between control and lesion rats. No difference between control and lesion rats was detected in the pSpike enhancement after PnO stimulation following scopolamine (5 mg/kg i.p.). Additional studies were needed to demonstrate the facilitation of dentate gyrus population spike by septohippocampal GABAergic neurons.

Related Products: Orexin-B-SAP (Cat. #IT-20)

Gaykema RP, Thacker GC, Shapiro NJ, Goehler LE (2009) Immunotoxic lesion of hypothalamic noradrenergic/adrenergic input ameliorates the effects of peripheral LPS challenge on sickness behavior and associated brain c-Fos expression. Neuroscience 2009 Abstracts 570.11/EE120. Society for Neuroscience, Chicago, IL.

Summary: Caudal medullary catecholamine neurons that innervate the hypothalamus play a major role in the activation of paraventricular neurons that drive pituitary adrenocorticotropin and adrenal corticosteroid release in response to peripheral pro-inflammatory challenges with interleukin-1 or lipopolysaccharide (LPS). Pro-inflammatory challenges also lead to marked behavioral changes, including fatigue, loss of social interest, anorexia, somnolence, but the precise neuronal mechanisms that underlie sickness behavior remain elusive. We reasoned that the medulla-hypothalamic catecholaminergic pathway may also contribute to the behavioral manifestations in illness. To investigate such possible role, we applied a targeted lesion approach in rats to determine whether or not caudal brainstem catecholaminergic neurons that innervate the hypothalamus are also necessary for the expression of sickness behavior. Anti-dopamine beta hydroxylase antibodies conjugated to saporin (DSAP), when injected into a target region, selectively poisons and destroy noradrenergic/adrenergic neurons that innervate the target. DSAP was micro-injected bilaterally into the hypothalamic paraventricular nucleus (PVN), whereas control rats received unconjugated saporin (SAP controls). Fourteen days later the animals were injected intraperitoneally with either LPS or saline, and 2h later were submitted to the open field to record their exploratory behavior, 1h after which the rats were sacrificed for brain immunohistochemical analyses. LPS-treated SAP control rats showed drastic reduction in exploratory behavior (reduced locomotion distance and velocity). Prior DSAP microinjections largely reversed the LPS-induced reduction in locomotor behavior. The brains of these DSAP rats showed a dramatic loss of noradrenergic innervation of the PVN but also in other parts of the medial, tuberal and tuberomammilary regions of the hypothalamus. The behavioral resilience to LPS coincided with diminished LPS-related c-Fos staining in the PVN, and increased c-Fos staining in the lateral and tuberomammillary regions related to behavior and/or arousal. In summary, our findings support the hypothesis that hypothalamic catecholaminergic projections originating in the lower brainstem play a critical role in the expression of sickness behavior in the context of novelty-induced exploratory activity, but we cannot determine with precision in which part of the hypothalamus the noradrenergic/adrenergic input contributes to the expression of sickness behavior due to extensive collateralization of the ascending projections throughout the hypothalamus.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Huang T-Y, Lin L-S, Chen H-I, Jen C (2009) Chronic treadmill exercise improves cerebellar functions: Alterations in mitochondrial protein expression, rotarod performance, and toxin resistance. Neuroscience 2009 Abstracts 660.18/CC34. Society for Neuroscience, Chicago, IL.

Summary: The effects of exercise on cerebellar functions were studied. Five-week-old male Wistar rats were divided into exercise and sedentary groups. For exercise groups, rats were subjected to 8 weeks of treadmill exercise at moderate intensity. In some groups, rats were administered with OX7-saporin, a cerebellar Purkinje cell toxin, into the lateral ventricle during the 5th week of training. At the end of training period, they were tested for rotarod performance. Brain tissues were obtained for measurement of mitochondria-related protein, including Mfn2, OPA1, Drp1 and CcOx-IV. The morphology of Purkinje cells was also examined by two photon microscopy. Our results showed that exercise training improve rotarod performance, and increased cerebellar protein levels of Mfn2 and OPA1 (mitochondrial fusion proteins) but not Drp1 (mitochondrial fission protein) or CcOx-IV (a mitochondrial complex IV marker). The dendritic field of Purkinje cells was significant modified in exercise groups. OX7-saporin application impaired the rotarod performance and decreased cerebellar Purkinje cell number only in sedentary rats. In summary, chronic exercise enlarged dendritic field of Purkinje cells and improved cerebellar function, including the rotarod performance, the mitochondrial fusion protein expression, and the resistance to toxin insult.

Related Products: OX7-SAP (Cat. #IT-02)

Carvalho AF, Reyes AS, Van Bockstaele EJ (2009) The role of limbic norepinephrine in cannabinoid-induced aversion. Neuroscience 2009 Abstracts 449.3/V29. Society for Neuroscience, Chicago, IL.

Summary: The endocannabinoid system has been implicated in diverse physiological mechanisms including modulation of pain and analgesia, learning and memory and feeding, among others. Thus, targeting the cannabinoid system has risen to the forefront in the development of novel treatments for a number of pathophysiological processes. Consistent with this, agonists of the cannabinoid receptor type 1 (CB1R) have been successfully used in the treatment of severe anorexia in patients with AIDS and in alleviating nausea and vomiting in patients undergoing chemotherapy. However, significant side effects have been observed in clinical trials raising concerns regarding the potential clinical utility of cannabinoid-based agents. Disturbances in mood and affect, including paranoia, anxiety and nervousness, have been reported in patients. Understanding the neural circuits and neurochemical substrates impacted by cannabinoids will provide a better means of gauging their actions within the central nervous system that contribute to the expression of unwanted side effects. We have previously shown an increase in anxiety-like behaviors in rats receiving repeated administration of cannabinoid agonists. This increase in anxiety was accompanied by increases in indices of noradrenergic activity. In the present study, we investigated whether norepinephrine in the limbic forebrain of rats is required for cannabinoid-induced aversion using an immunotoxin lesion approach combined with behavioral analysis using a place conditioning paradigm. Male Sprague Dawley rats received bilateral injections of a ribosomal toxin, saporin (SAP) conjugated to an antibody that specifically recognizes the enzyme dopamine-beta-hydroxylase (DSAP), into the limbic forebrain. Control rats received saporin alone. As previously reported, administration of the synthetic cannabinoid receptor agonist, WIN 55,212-2 (3.0mg/kg), induced aversion in a place conditioning paradigm in SAP-only treated rats. The rats’ spatial memory was also evaluated using the Morris Water Maze. Depletion of norepinephrine using DSAP in specific limbic regions impaired cannabinoid-induced aversion to WIN 55,212-2 without affecting learning and memory processes. Taken together, noradrenergic projections to the limbic forebrain may be critical in the manifestation of aversive behaviors associated with cannabinoid agonist exposure.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Freiria-Oliveira AH, Blanch GT, De Paula PM, Colombari E, Menani JV, Colombari DS (2009) Role of A2 noradrenergic neurons and angiotensinergic mechanisms on hypotension induced by hemorrhage. Neuroscience 2009 Abstracts 467.18/DD70. Society for Neuroscience, Chicago, IL.

Summary: The A2 catecholaminergic neurons in the commissural subdivision of the nucleus tractus solitarii (cNTS) are activated by hemorrhage. However, the role of these neurons on the cardiovascular adjustments to hemorrhage is not fully understood. In the present study we investigated the effects of A2 noradrenergic neuron lesion alone or combined with the blockade of angiotensinergic mechanisms on the recovery of blood pressure after hemorrhage. Male Holtzman rats (280-320 g) anesthetized with ketamine combined with xylazine were submitted to lesions of dopamine-beta-hydroxilase (DβH)-containing neurons in the cNTS achieved with injections of anti-DβH-saporin (12.6 ng/60 nl, n=6-8) or sham lesions (injection of immunoglobulin-G-saporin, 12.6 ng/60 nl, n=6). Changes in blood pressure to hemorrhage were tested 30 days after lesions. Immunohistochemistry for tyrosine-hydroxilase was performed to confirm the efficacy of DβH neuron lesion in the cNTS. Two days before tests, femoral artery and vein were cannulated under ketamine and xylazine anesthesia. Hemorrhage consisted in four blood withdrawals (2 ml/300 g body weight, every 10 min) in conscious rats. Immediately after the 4th blood withdrawal, the hypotension was similar in A2-lesioned and sham-lesioned rats (-62 ± 7 mmHg and -73±7 mmHg, respectively). However, A2-lesioned rats rapidly (20 min) recovered from hypotension (-7±2 mmHg), while sham rats did not completely recover from hypotension until the end of experiment (60 min after the 4th blood withdrawn, -20±3 mmHg). The pre-treatment with losartan (angiotensin type 1 receptor antagonist, 10 mg/kg of body weight, iv) impaired the recovery of blood pressure by A2-lesioned rats (-29 ± 4 mmHg and -28 ± 3 mmHg, 20 and 60 min after the 4th blood withdrawal). In sham rats, the treatment with losartan also reduced the partial recovery of blood pressure at the end of the test (-39±6 mmHg, vs. sham control: -20±3mmHg), however, losartan did not affect the hypotension 20 min after the 4th blood withdrawal (-30± 6 mmHg vs. sham control: -35 ± 9 mmHg). The results suggest that A2 noradrenergic neuron lesion in the cNTS facilitates the recovery of hypotension after hemorrhage, probably increasing the action of angiotensinergic mechanisms.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Macleod JE, Ackerman CM, Bucci DJ (2009) Contributions of the medial prefrontal cortex to negative occasion setting. Neuroscience 2009 Abstracts 477.2/FF61. Society for Neuroscience, Chicago, IL.

Summary: The medial prefrontal cortex of rats has a role in many aspects of cognitive function, and especially forms of inhibitory learning. Recent research has revealed heterogeneous functions of the prelimbic (PL) and infralimbic (IL) regions of the medial prefrontal cortex in modulating response inhibition. In a recent study, we tested the effects of separate neurotoxic lesions of the PL or IL in a serial feature negative discrimination paradigm (negative occasion setting). Rats received daily training sessions consisting of 16 trials: on 4 trials in each session, a tone was presented and followed by food reward; on the remaining trials, the tone was preceded by a visual stimulus and not reinforced. Our results indicate that PL but not IL is necessary for learning the discrimination. A second study was conducted to investigate the effects of these lesions on rats that were first extensively trained in this task. We found that rats that had been trained for 30 days prior to receiving PL or IL lesions were still able to perform the task as well as controls. Therefore, PL lesions disrupt acquisition but not performance of a serial feature negative discrimination. This same task has been used in our laboratory to investigate the effects of nicotine on learning. We have shown that nicotine-treated rats exhibit greater discrimination between the two trial types as evidenced by less frequent responding during non-reinforced trials, and learn the discrimination in fewer sessions than control rats. In addition, rats receiving nicotine showed an increase in rearing behavior during the presentation of the light, suggesting nicotine enhanced attention to the visual stimulus. One possible critical site of action for nicotine’s effects is the medial prefrontal cortex. Research in other laboratories utilizing other training procedures suggest that cholinergic activity in the medial prefrontal cortex is critical for attending to behaviorally relevant stimuli, and have implicated the rat PL in visual attention as well as inhibiting prepotent, goal oriented responses. We investigated the contribution of the cholinergic PL to learning the serial feature negative discrimination task by training rats that had received infusions of 192-IgG-saporin into PL to remove cholinergic input from the basal forebrain. No differences between control and lesion rats were observed. Taken together, the results suggest that PL is necessary for acquisition of a serial feature negative discrimination, although the basal forebrain cholinergic input into this region is not required to sufficiently learn the task.

Related Products: 192-IgG-SAP (Cat. #IT-01)