control-conjugates

Hovinga KE, Shimizu F, Wang R, Panagiotakos G, Van Der Heijden M, Moayedpardazi H, Correia AS, Soulet D, Major T, Menon J, Tabar V (2010) Inhibition of notch signaling in glioblastoma targets cancer stem cells via an endothelial cell intermediate. Stem Cells 28:1019-1029. doi: 10.1002/stem.429 PMID: 20506127

Summary: The antibody CD105 (1:1,000) was incubated with Mab-ZAP, to allow binding and formation of a CD105 antibody-saporin complex, which was added to explants or to a human cerebral microvessel endothelial cell line as control.  CD105 antibody was also incubated with a Goat-IgG-SAP for control  that does not bind CD105.  The conjugates were injected into the explant under a dissecting microscope after gently incising the explant surface for better access.

Related Products: Mab-ZAP (Cat. #IT-04), Goat IgG-SAP (Cat. #IT-19)

Vianna DM, Carrive P (2010) Cardiovascular and behavioural responses to conditioned fear and restraint are not affected by retrograde lesions of A5 and C1 bulbospinal neurons. Neuroscience 166:1210-1218. doi: 10.1016/j.neuroscience.2010.01.039

Summary: To investigate the role of A5 neurons in some forms of psychological stress the authors injected 22 or 44 ng of anti-DBH-SAP (Cat. #IT-03) into the spinal cord of rats. Mouse IgG-SAP (Cat. #IT-18) was used as a control. The data shows that A5 presympathetic neurons are not essential for the expression of the tachycardic and pressor responses to conditioned fear and restraint.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18)

Dailey MJ, Bartness TJ (2010) Arcuate nucleus destruction does not block food deprivation-induced increases in food foraging and hoarding. Brain Res 1323:94-108. doi: 10.1016/j.brainres.2010.01.078

Summary: While some aspects of food intake are understood, mechanisms controlling hoarding of food have not been identified. This work investigates the role of NPY in the arcuate nucleus (Arc) in hoarding. Siberian hamsters received 48 ng injections of NPY-SAP (Cat. #IT-28) into the Arc; blank-SAP (Cat. #IT-21) was used as a control. In lesioned animals food deprivation-induced hoarding was increased 100%, but baseline foraging and food hoarding was unchanged.

Related Products: NPY-SAP (Cat. #IT-28), Blank-SAP (Cat. #IT-21)

Li AJ, Wang Q, Dinh TT, Ritter S (2010) Featured Article: Deletion of NPY/AGRP and POMC neurons in the arcuate nucleus by leptin-saporin produces hyperphagia, obesity and changes in diurnal feeding patterns in rats. Targeting Trends 11(1)

Related Products: Leptin-SAP (Cat. #IT-47), Blank-SAP (Cat. #IT-21)

Read the featured article in Targeting Trends.

See Also:

• Li A-J et al. Leptin-saporin injection into the arcuate nucleus lesions NPY/AGRP and POMC neurons and produces hyperphagia, obesity and changes in diurnal feeding patterns in rats. Neuroscience 2009 Abstracts 374.5/EE116, 2009. Society for Neuroscience, Chicago, IL

Chen ZF, Sun YG, Zhao ZQ, Meng XL, Yin J, Liu XY (2009) Featured Article: Ablation of GRPR+ neurons in the spinal cord by bombesin-saporin knocks out itch sensation in mice without affecting pain circuit. Targeting Trends 10(4)

Related Products: Bombesin-SAP (Cat. #IT-40), Blank-SAP (Cat. #IT-21)

Read the featured article in Targeting Trends.

See Also:

• Sun YG et al. Cellular basis of itch sensation. Science 325:1531-1534, 2009.

Jinks SL, Carstens E, Antognini JF (2009) Nitrous oxide-induced analgesia does not influence nitrous oxide’s immobilizing requirements. Anesth Analg 109:1111-1116. doi: 10.1213/ANE.0b013e3181b5a2a7

Summary: Noradrenergic neurons in the locus coeruleus (LC) are involved with the analgesic action of nitrous oxide (N2O). In order to examine whether these neurons are also involved with the immobilizing effects of N2O, rats received 4 µg intracerebroventricular injections of anti-DBH-SAP (Cat. #IT-03). Mouse IgG-SAP (Cat. #IT-18) was used as a control. Lesioned animals did not experience the analgesic effects of N2O, but the immobilizing effects were still present. The data demonstrate that the immobilizing mechanism of N2O is independent from its analgesic effects.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18)

Dashniani M, Burjanadze M, Beselia G, Maglakelidze G, Naneishvili T (2009) Spatial memory following selective cholinergic lesion of the nucleus basalis magnocellularis. Georgian Med News 174:77-81.

Summary: This study investigated the role of cholinergic nucleus basalis magnocellularis (NBM) cells in learning and memory. Rats received bilateral 200 ng injections of 192 IgG-SAP (Cat. IT-01) into the NBM. Mouse IgG-SAP (Cat. #IT-18) was used as a control. The results indicate that the NBM is important in accurate spatial learning and the processing of information about the spatial environment. Deficits in rats with the cholinergic lesion may be due to lowered attentional function.

Related Products: 192-IgG-SAP (Cat. #IT-01), Mouse IgG-SAP (Cat. #IT-18)

Zhang L, Moffatt-Bruce SD, Gaughan AA, Wang JJ, Rajab A, Hadley GA (2009) An anti-CD103 immunotoxin promotes long-term survival of pancreatic islet allografts. Am J Transplant 9:2012-2023. doi: 10.1111/j.1600-6143.2009.02735.x PMID: 19645708

Objective: To determine whether blockade of the CD103 pathway represents a viable therapeutic strategy for intervention in organ allograft rejection and graft-vs-host disease.

Summary: These data document that depletion of CD103 expressing cells represents a viable strategy for therapeutic intervention in allograft rejection.

Usage: Recipient C57BL/6 mice received 2.0 mg/kg M290-SAP or Rat IgG-SAP on day 3 post-transplantation.

Related Products: Anti-CD103-SAP (Cat. #IT-50), Rat IgG-SAP (Cat. #IT-17)

Wiley RG, Lemons LL, Kline IV RH (2009) Neuropeptide Y receptor-expressing dorsal horn neurons: role in nocifensive reflex responses to heat and formalin. Neuroscience 161:139-147. doi: 10.1016/j.neuroscience.2008.12.017

Summary: This work examines the effect of lumbar intrathecal administration of NPY-SAP (Cat. #IT-28), and the role of Y1 NPY receptor-expressing neurons (Y1R) in response to thermal and chemical stimulation. Rats received 500 ng or 750 ng intrathecal injections of NPY-SAP. Blank-SAP (Cat. #IT-21) was used as a control. Lesioned animals displayed a specific loss of Y1R in the dorsal horn, as well as reduced nocifensive reflex responses.

Related Products: NPY-SAP (Cat. #IT-28), Blank-SAP (Cat. #IT-21)

Ray AP, Chebolu S, Ramirez J, Darmani NA (2009) Ablation of least shrew central neurokinin NK1 receptors reduces GR73632-induced vomiting. Behav Neurosci 123:701-706. doi: 10.1037/a0015733

Summary: In this work the authors investigated the role of central and peripheral nervous systems components that mediate the emetic reflex. Least shrews received an 600-ng injection of SSP-SAP (Cat. #IT-11) into the lateral ventricle. Some animals also received a 4.8-µg intraperitoneal injection of SSP-SAP. Blank-SAP (Cat. #IT-21) and unconjugated saporin (Cat. #PR-01) were used as controls. Lesioned animals displayed reduced emesis, but the data indicate that a minor peripheral nervous system component is also present.

Related Products: SSP-SAP (Cat. #IT-11), Blank-SAP (Cat. #IT-21), Saporin (Cat. #PR-01)