control-conjugates

Dashniani M, Burjanadze M, Beselia G, Maglakelidze G, Naneishvili T (2009) Spatial memory following selective cholinergic lesion of the nucleus basalis magnocellularis. Georgian Med News 174:77-81.

Summary: This study investigated the role of cholinergic nucleus basalis magnocellularis (NBM) cells in learning and memory. Rats received bilateral 200 ng injections of 192 IgG-SAP (Cat. IT-01) into the NBM. Mouse IgG-SAP (Cat. #IT-18) was used as a control. The results indicate that the NBM is important in accurate spatial learning and the processing of information about the spatial environment. Deficits in rats with the cholinergic lesion may be due to lowered attentional function.

Related Products: 192-IgG-SAP (Cat. #IT-01), Mouse IgG-SAP (Cat. #IT-18)

Zhang L, Moffatt-Bruce SD, Gaughan AA, Wang JJ, Rajab A, Hadley GA (2009) An anti-CD103 immunotoxin promotes long-term survival of pancreatic islet allografts. Am J Transplant 9:2012-2023. doi: 10.1111/j.1600-6143.2009.02735.x PMID: 19645708

Objective: To determine whether blockade of the CD103 pathway represents a viable therapeutic strategy for intervention in organ allograft rejection and graft-vs-host disease.

Summary: These data document that depletion of CD103 expressing cells represents a viable strategy for therapeutic intervention in allograft rejection.

Usage: Recipient C57BL/6 mice received 2.0 mg/kg M290-SAP or Rat IgG-SAP on day 3 post-transplantation.

Related Products: Anti-CD103-SAP (Cat. #IT-50), Rat IgG-SAP (Cat. #IT-17)

Wiley RG, Lemons LL, Kline IV RH (2009) Neuropeptide Y receptor-expressing dorsal horn neurons: role in nocifensive reflex responses to heat and formalin. Neuroscience 161:139-147. doi: 10.1016/j.neuroscience.2008.12.017

Summary: This work examines the effect of lumbar intrathecal administration of NPY-SAP (Cat. #IT-28), and the role of Y1 NPY receptor-expressing neurons (Y1R) in response to thermal and chemical stimulation. Rats received 500 ng or 750 ng intrathecal injections of NPY-SAP. Blank-SAP (Cat. #IT-21) was used as a control. Lesioned animals displayed a specific loss of Y1R in the dorsal horn, as well as reduced nocifensive reflex responses.

Related Products: NPY-SAP (Cat. #IT-28), Blank-SAP (Cat. #IT-21)

Ray AP, Chebolu S, Ramirez J, Darmani NA (2009) Ablation of least shrew central neurokinin NK1 receptors reduces GR73632-induced vomiting. Behav Neurosci 123:701-706. doi: 10.1037/a0015733

Summary: In this work the authors investigated the role of central and peripheral nervous systems components that mediate the emetic reflex. Least shrews received an 600-ng injection of SSP-SAP (Cat. #IT-11) into the lateral ventricle. Some animals also received a 4.8-µg intraperitoneal injection of SSP-SAP. Blank-SAP (Cat. #IT-21) and unconjugated saporin (Cat. #PR-01) were used as controls. Lesioned animals displayed reduced emesis, but the data indicate that a minor peripheral nervous system component is also present.

Related Products: SSP-SAP (Cat. #IT-11), Blank-SAP (Cat. #IT-21), Saporin (Cat. #PR-01)

Radley JJ, Gosselink KL, Sawchenko PE (2009) A discrete GABAergic relay mediates medial prefrontal cortical inhibition of the neuroendocrine stress response. J Neurosci 29:7330-7340. doi: 10.1523/JNEUROSCI.5924-08.2009

Summary: GABAergic neurons have been implicated in the negative regulation of the hypothalamic-pituitary-adrenal axis (HPA). In order to clarify GABAergic input to the paraventricular hypothalamic nucleus the authors injected 0.23 µg of GAT1-SAP (Cat. #IT-32) into the anterior bed nucleus of the stria terminalis. Both unilateral and bilateral injections were used. Rabbit IgG-SAP (Cat. #IT-35) was used as a control. The data indicate that the GABAergic neuronal population functions as proximate mediator of HPA-inhibitory limbic influences.

Related Products: GAT1-SAP (Cat. #IT-32), Rabbit IgG-SAP (Cat. #IT-35)

Brightwell JJ, Taylor BK (2009) Noradrenergic neurons in the locus coeruleus contribute to neuropathic pain. Neuroscience 160:174-185. doi: 10.1016/j.neuroscience.2009.02.023

Summary: Noradrenergic neurons were eliminated with 5 µg intracerebroventricular injections of anti-DBH-SAP (Cat. #IT-03). Mouse IgG-SAP (Cat. #IT-18) was used as a control. Animals lesioned with anti-DBH-SAP displayed a reduction in behavioral signs of several kinds of allodynia.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18)

Khan AM, Rapp KL, Ponzio TA, Sanchez-Watts G, Watts AG (2009) Featured Article: Deletion of catecholaminergic neurons by anti-DBH-saporin disrupts hypothalamic MAP kinase and CREB activation. Targeting Trends 10(2)

Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18)

Read the featured article in Targeting Trends.

See Also:

• Khan AM et al. Stimulus-, circuit- and intracellular-level determinants of MAP kinase and CREB activation in parvicellular hypothalamic paraventricular neurons. Neuroscience 2008 Abstracts 865.23/MM24, 2008. Society for Neuroscience, Washington, DC

Dashniani MG, Beseliia GV, Maglakelidze GA, Burdzhanadze MA, Chkhikvishvili N (2009) Effects of the selective lesions of cholinergic septohippocampal neurons on different forms of memory and learning process. Georgian Med News 166:81-85.

Summary: The hippocampus is crucial for the ability to recollect everyday events and factual knowledge. Here the authors looked at the role of the septo-hippocampal cholinergic system of the medial septum in learning and memory. Rats received 200 ng injections of 192-IgG-SAP (Cat. #IT-01) into the medial septum. Mouse IgG-SAP (Cat. #IT-18) was used as a control. The data suggest that although the septo-hippocampal region is essential for spatial learning, hippocampal acetyl cholinesterase may not be essential for all types of hippocampal-dependent memory.

Related Products: 192-IgG-SAP (Cat. #IT-01), Mouse IgG-SAP (Cat. #IT-18)

Loyd DR, Wang X, Murphy AZ (2008) Sex differences in micro-opioid receptor expression in the rat midbrain periaqueductal gray are essential for eliciting sex differences in morphine analgesia. J Neurosci 28:14007-14017. doi: 10.1523/JNEUROSCI.4123-08.2008

Summary: The authors test whether the periaqueductal gray (PAG), that contains a dense population of µ-opioid receptor (MOR)-expressing neurons, is sexually dimorphic. Rats were injected with 3 pmol of Dermorphin-SAP (Cat. #IT-12) into the PAG. Blank-SAP (Cat. #IT-21) was used as a control. Both behavioral and immunohistochemical evidence suggest that differential expression of MOR-expressing neurons in the PAG between male and female rats accounts for the difference in response to morphine.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Blank-SAP (Cat. #IT-21)

Rouleau C, Curiel M, Weber W, Smale R, Kurtzberg L, Mascarello J, Berger C, Wallar G, Bagley R, Honma N, Hasegawa K, Ishida I, Kataoka S, Thurberg BL, Mehraein K, Horten B, Miller G, Teicher BA (2008) Endosialin protein expression and therapeutic target potential in human solid tumors: sarcoma versus carcinoma. Clin Cancer Res 14:7223-7236. doi: 10.1158/1078-0432.CCR-08-0499

Summary: Endosialin is an antigen expressed in many human cancer cell lines. As part of a wide-ranging study investigating clinical specimens, cell culture, and animal models, this group used Hum-ZAP (Cat. #IT-22) combined with a humanized anti-endosialin antibody in cell proliferation assays. Mouse IgG-SAP (Cat. #IT-18) was used as a control. The anti-endosialin antibody and Hum-ZAP were incubated together in equimolar concentrations then applied to cells in culture. Various cancers, including synovial sarcoma, fibrosarcoma, and osteosarcoma among others, were found to express endosialin.

Related Products: Hum-ZAP (Cat. #IT-22), Mouse IgG-SAP (Cat. #IT-18)