antibodies

Cassandras M, Wang C, Kathiriya J, Tsukui T, Matatia P, Matthay M, Wolters P, Molofsky A, Sheppard D, Chapman H, Peng T (2020) Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nat Cell Biol 22(11):1295-1306. doi: 10.1038/s41556-020-00591-9 PMID: 33046884

Objective: To show that Gli1+ mesenchymal stromal cells (MSCs) promote metaplastic differentiation of airway progenitors into KRT5+ basal cells.

Summary: Gli1+ MSCs integrate hedgehog signaling as a rheostat to control BMP activation in the progenitor niche to determine regenerative outcome in fibrosis.

Usage: Histology and Immunofluorescence (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Cops J, De Moor B, Haesen S, Lijnen L, Wens I, Lemoine L, Reynders C, Penders J, Lambrichts I, Mullens W, Hansen D (2020) Endurance exercise intervention is beneficial to kidney function in a rat model of isolated abdominal venous congestion: A pilot study. J Cardiovasc Transl Res 13(5):769-782. doi: 10.1007/s12265-019-09947-5 PMID: 31848881

Objective: To the effects of moderate intense endurance exercise on heart and kidney function and morphology in a rat model of abdominal venous congestion.

Summary: The implementation of moderate intense endurance exercise reduces abdominal venous pressure and is beneficial to kidney function.

Usage: estern blot; to investigate whole kidney renin-angiotensin-aldosterone system (RAAS) protein expression, the membrane was incubated overnight at 4 °C in the presence of AT-1R antibody 1:2000

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Feng X, Guo Q, Xue H, Duan X, Jin S, Wu Y (2020) Hydrogen sulfide attenuated Angiotensin II-induced sympathetic excitation in offspring of renovascular hypertensive rats. Front Pharmacol 11:565726. doi: 10.3389/fphar.2020.565726 PMID: 33041805

Objective: To whether sensitivity to angiotensin II (Ang II) is enhanced in offspring of renovascular hypertensive animals and whether hydrogen sulfide (H2S) can attenuate the increased response to Ang II in offspring.

Summary: Prenatal or postnatal administration of H2S attenuated AngII-induced sympathetic excitation in offspring of hypertensive rats.

Usage: Western blot (1:800)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Lee SB, Ko A, Oh YT, Shi P, D’Angelo F, Frangaj B, Koller A, Chen EI, Cardozo T, Iavarone A, Lasorella A (2020) Proline hydroxylation primes protein kinases for autophosphorylation and activation. Mol Cell 79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021 PMID: 32640193

Objective: To investigate the formation of prolyl-hydroxylated intermediates as a novel mechanism of kinase maturation and a general mechanism of regulation of CMGC kinases in eukaryotes.

Summary: Identified a highly conserved proline in the kinase domain of CMGC kinases that is hydroxylated by the PHD1 prolyl-hydroxylase, and this event precedes tyrosine autophosphorylation.

Usage: Western blot

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Kucharczyk MW, Chisholm KI, Denk F, Dickenson AH, Bannister K, McMahon SB (2020) The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli. Pain 161(8):1894-1905. doi: 10.1097/j.pain.0000000000001880 PMID: 32701848

Usage: immunohistochemistry (1:400)

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Zhao L, Wang J, Zhang Y, Wang L, Yu M, Wang F (2020) Vitamin C decreases VEGF expression levels via hypoxia‑inducible factor‑1α dependent and independent pathways in lens epithelial cells. Mol Med Rep 22(1):436-444. doi: 10.3892/mmr.2020.11103 PMID: 32377733

Objective: To investigate the mechanisms underlying the effects of vitamin C on the expression levels of VEGF.

Summary: Vitamin C inhibits the cell proliferation and the expression levels of VEGF in lens epithelial cells following a co-treatment with the HIF-1 inhibitor.

Usage: Western blot (1:500)

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Kim M, Do GY, Kim I (2020) Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome. Korean J Physiol Pharmacol 24(4):319-328. doi: 10.4196/kjpp.2020.24.4.319 PMID: 32587126

Objective: To investigate the hypothesis that high fructose intake induces activation of the renin-angiotensin systems (RAS), resulting in hypertension and metabolic syndrome.

Summary: High fructose intake increased serum renin, Ang II, triglyceride, and cholesterol levels. High fructose intake increased the expression of angiotensinogen in the liver; angiotensin-converting enzyme in the lungs; and renin, angiotensin II type 1a receptor (AT1aR), and angiotensin II type 1b receptor (AT1bR) in the kidneys. High fructose intake induces activation of RAS, resulting in hypertension and metabolic syndrome.

Usage: Immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

da Rocha ES, Gomes FC, de Oliveira MR, Bruneto RV, Borges RMR, de Carvalho EP, Angeli-Junior EV, Garcia-Filho GF, Doná JPL, de Oliveira Batanero RP, Carlos CP, de Melo-Neto JS (2020) Effects of adjuvant-induced arthritis on the ventral prostate of rats treated with angiotensin AT1 receptor blocker. Life Sci 252:117629. doi: 10.1016/j.lfs.2020.117629 PMID: 32247619

Objective: To analyze the prostatic compartments, extracellular matrix, microvascularization, transforming growth factor-beta (TGF-β) and angiotensin II receptors type 1 (AT1) levels, and histopathology of the ventral prostate in a rat model for rheumatoid arthritis, and to evaluate the effect of angiotensin II AT1 receptor blocker (ARB) in the disease.

Summary: AIA may activate specific mechanisms that modify the prostatic environment; the use of ARB attenuates some altered prostate parameters in a rat model for arthritis.

Usage: Immunohistochemistry (1:500)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Zhao Y, Ge X, Yu H, Kuil LE, Alves MM, Tian D, Huang Q, Chen X, Hofstra RMW, Gao Y (2020) Inhibition of ROCK signaling pathway accelerates enteric neural crest cell-based therapy after transplantation in a rat hypoganglionic model. Neurogastroenterol Motil 32(9):e13895. doi: 10.1111/nmo.13895 PMID: 32515097

Objective: To test the hypothesis that modulation of extracellular factors during transplantation could promote enteric neural crest cells (ENCCs) proliferation and migration, leading to increased transplantation efficiency.

Summary: Since the RhoA/ROCK pathway is highly involved in cytoskeletal dynamics and neurite growth, this study explored the effect of inhibition of this pathway to improve the success of ENCCs transplantation. inhibition of ROCK increased viable cell number, differentiation, and migration of ENCCs in vitro. Moreover, transplantation of labeled ENCCs into the hypoganglionic model showed enhanced distribution of grafted ENCCs, upon treatment with ROCK inhibitor.

Usage: Immunofluorescence (1:200); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Jani MS, Zou J, Veetil AT, Krishnan Y (2020) A DNA-based fluorescent probe maps NOS3 activity with subcellular spatial resolution. Nat Chem Biol 16:660–666. doi: 10.1038/s41589-020-0491-3 PMID: 32152543

Objective: Usage of novel fluorescent DNA-based probe technology to map activities of endogenous NOS3 in live cells provides a platform to discover selective regulators of NOS3 in PM and TGN.

Summary: Targeting of plasma membrane (PM) and trans-Golgi network (TGN) shows that NOS3 is tenfold less active at the Golgi but its activity is essential for the structural integrity of the Golgi.

Usage: immunofluorescence

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