antibodies

Wang Z, Wu J, Hu Z, Luo C, Wang P, Zhang Y, Li H (2020) Dexmedetomidine alleviates lipopolysaccharide-induced acute kidney injury by inhibiting p75NTR-mediated oxidative stress and apoptosis. Oxid Med Cell Longev 2020:5454210. doi: 10.1155/2020/5454210 PMID: 33194004

Objective: To study antioxidant effects and the mechanism of Dexmedetomidine (DEX) in an inflammatory proximal tubular epithelial cell model and lipopolysaccharide- (LPS-) induced AKI in mice.

Summary: DEX ameliorated AKI in mice with sepsis by partially reducing oxidative stress and apoptosis through regulation of p75NTR/p38MAPK/JNK signaling pathways.

Usage: Western blot (1:5000); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kim HY, Kim HS (2021) Sulfatase 1 mediates IL-10-induced dimethylarginine dimethylaminohydrolase-1 expression and antiproliferative effects in vascular smooth muscle cells of spontaneously hypertensive rats. Cytokine 137:155344. doi: 10.1016/j.cyto.2020.155344 PMID: 33128921

Objective: To examine the effects of exSulfs on IL-10-induced dimethylarginine dimethylaminohydrolase-1 (DDAH-1) expression, abrogation of Ang II-induced DDAH-1 downregulation, and inhibition of Ang II-induced proliferation of spontaneously hypertensive rats (SHRs) vascular smooth muscle cells (VSMC). IL-10-induced DDAH-1 expression and abrogation of Ang II-induced DDAH-1 downregulation were attenuated in Sulf1 siRNA-transfected SHRs VSMC.

Summary: Sulf1, and not Sulf2, mediates the IL-10-induced inhibition of Ang II-induced hypertensive effects in SHRs VSMC.

Usage: Western blot (1:800)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Cassandras M, Wang C, Kathiriya J, Tsukui T, Matatia P, Matthay M, Wolters P, Molofsky A, Sheppard D, Chapman H, Peng T (2020) Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nat Cell Biol 22(11):1295-1306. doi: 10.1038/s41556-020-00591-9 PMID: 33046884

Objective: To show that Gli1+ mesenchymal stromal cells (MSCs) promote metaplastic differentiation of airway progenitors into KRT5+ basal cells.

Summary: Gli1+ MSCs integrate hedgehog signaling as a rheostat to control BMP activation in the progenitor niche to determine regenerative outcome in fibrosis.

Usage: Histology and Immunofluorescence (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Cops J, De Moor B, Haesen S, Lijnen L, Wens I, Lemoine L, Reynders C, Penders J, Lambrichts I, Mullens W, Hansen D (2020) Endurance exercise intervention is beneficial to kidney function in a rat model of isolated abdominal venous congestion: A pilot study. J Cardiovasc Transl Res 13(5):769-782. doi: 10.1007/s12265-019-09947-5 PMID: 31848881

Objective: To the effects of moderate intense endurance exercise on heart and kidney function and morphology in a rat model of abdominal venous congestion.

Summary: The implementation of moderate intense endurance exercise reduces abdominal venous pressure and is beneficial to kidney function.

Usage: estern blot; to investigate whole kidney renin-angiotensin-aldosterone system (RAAS) protein expression, the membrane was incubated overnight at 4 °C in the presence of AT-1R antibody 1:2000

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Feng X, Guo Q, Xue H, Duan X, Jin S, Wu Y (2020) Hydrogen sulfide attenuated Angiotensin II-induced sympathetic excitation in offspring of renovascular hypertensive rats. Front Pharmacol 11:565726. doi: 10.3389/fphar.2020.565726 PMID: 33041805

Objective: To whether sensitivity to angiotensin II (Ang II) is enhanced in offspring of renovascular hypertensive animals and whether hydrogen sulfide (H2S) can attenuate the increased response to Ang II in offspring.

Summary: Prenatal or postnatal administration of H2S attenuated AngII-induced sympathetic excitation in offspring of hypertensive rats.

Usage: Western blot (1:800)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Lee SB, Ko A, Oh YT, Shi P, D’Angelo F, Frangaj B, Koller A, Chen EI, Cardozo T, Iavarone A, Lasorella A (2020) Proline hydroxylation primes protein kinases for autophosphorylation and activation. Mol Cell 79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021 PMID: 32640193

Objective: To investigate the formation of prolyl-hydroxylated intermediates as a novel mechanism of kinase maturation and a general mechanism of regulation of CMGC kinases in eukaryotes.

Summary: Identified a highly conserved proline in the kinase domain of CMGC kinases that is hydroxylated by the PHD1 prolyl-hydroxylase, and this event precedes tyrosine autophosphorylation.

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Kucharczyk MW, Chisholm KI, Denk F, Dickenson AH, Bannister K, McMahon SB (2020) The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli. Pain 161(8):1894-1905. doi: 10.1097/j.pain.0000000000001880 PMID: 32701848

Usage: immunohistochemistry (1:400)

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Zhao L, Wang J, Zhang Y, Wang L, Yu M, Wang F (2020) Vitamin C decreases VEGF expression levels via hypoxia‑inducible factor‑1α dependent and independent pathways in lens epithelial cells. Mol Med Rep 22(1):436-444. doi: 10.3892/mmr.2020.11103 PMID: 32377733

Objective: To investigate the mechanisms underlying the effects of vitamin C on the expression levels of VEGF.

Summary: Vitamin C inhibits the cell proliferation and the expression levels of VEGF in lens epithelial cells following a co-treatment with the HIF-1 inhibitor.

Usage: Western blot (1:500)

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Kim M, Do GY, Kim I (2020) Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome. Korean J Physiol Pharmacol 24(4):319-328. doi: 10.4196/kjpp.2020.24.4.319 PMID: 32587126

Objective: To investigate the hypothesis that high fructose intake induces activation of the renin-angiotensin systems (RAS), resulting in hypertension and metabolic syndrome.

Summary: High fructose intake increased serum renin, Ang II, triglyceride, and cholesterol levels. High fructose intake increased the expression of angiotensinogen in the liver; angiotensin-converting enzyme in the lungs; and renin, angiotensin II type 1a receptor (AT1aR), and angiotensin II type 1b receptor (AT1bR) in the kidneys. High fructose intake induces activation of RAS, resulting in hypertension and metabolic syndrome.

Usage: Immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

da Rocha ES, Gomes FC, de Oliveira MR, Bruneto RV, Borges RMR, de Carvalho EP, Angeli-Junior EV, Garcia-Filho GF, Doná JPL, de Oliveira Batanero RP, Carlos CP, de Melo-Neto JS (2020) Effects of adjuvant-induced arthritis on the ventral prostate of rats treated with angiotensin AT1 receptor blocker. Life Sci 252:117629. doi: 10.1016/j.lfs.2020.117629 PMID: 32247619

Objective: To analyze the prostatic compartments, extracellular matrix, microvascularization, transforming growth factor-beta (TGF-β) and angiotensin II receptors type 1 (AT1) levels, and histopathology of the ventral prostate in a rat model for rheumatoid arthritis, and to evaluate the effect of angiotensin II AT1 receptor blocker (ARB) in the disease.

Summary: AIA may activate specific mechanisms that modify the prostatic environment; the use of ARB attenuates some altered prostate parameters in a rat model for arthritis.

Usage: Immunohistochemistry (1:500)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)