antibodies

Barnett SC, Alexander CL Iwashita Y, Gilson JM, Crowther J, Clark L, Dunn KT, Papanastassiou V, Kennedy PG, Franklin RJ (2000) Identification of a human olfactory ensheathing cell that can effect transplant mediated remyelination of demyelinated CNS axons. Brain 123:1581-1588. doi: 10.1093/brain/123.8.1581 PMID: 10908188

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Tremere LA, Pinaud R, Grosche J, Hartig W, Rasmusson DD (2000) Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species. NeuroReport 11(10):2177-2183. doi: 10.1097/00001756-200007140-00023 PMID: 10923666

Summary: 192-SAP (Cat. #IT-01) is a highly successful reagent for eliminating cholinergic neurons in rats. Because the targeting antibody only recognizes rat p75, it is unable to be used in other species. Tremere et al. have stained basal forebrain sections with ME20.4, a monoclonal antibody to human p75 (Cat. #AB-N07) and found excellent cross-reactivity in dog, raccoon, cat, pig and rabbit. The authors state that an ME20.4-saporin conjugate could be used to produce cholinergic basal forebrain lesions in several species.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07), 192-IgG-SAP (Cat. #IT-01)

Lorch SA, Foust R 3rd, Gow A, Arkovitz M, Salzman AL, Szabo C, Vayert B, Geffard M, Ischiropoulos H (2000) Immunohistochemical localization of protein 3-nitrotyrosine and S-nitrosocysteine in a murine model of inhaled nitric oxide therapy. Pediatr Res 47(6):798-805. doi: 10.1203/00006450-200006000-00020 PMID: 10832741

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Rossner S, Schliebs R, Bigl V (2000) Intracerebroventricular infusion of CHO5, a rat monoclonal antibody directed against mouse low-affinity nerve growth factor receptor (p75NTR), specifically labels basal forebrain cholinergic neurons in mouse brain. Metab Brain Dis 15(1):17-27. doi: 10.1007/BF02680011 PMID: 10885538

Summary: 192-Saporin (Cat. #IT-01) has long been a useful tool for neurobiological research in the rat. For various reasons, many researchers want to perform the same studies in the mouse but have been prevented from doing so by the lack of a suitable antibody against the mouse p75NTR. Rossner et al. describe a rat monoclonal antibody against the mouse p75NTR (Cat. #AB-N02) that demonstrates co-localization of p75NTR and ChAT, and also co-localization of p75NTR and TrkA in the mouse basal forebrain. Internalization and retrograde transport of this antibody in cholinergic basal forebrain neurons is also shown. This evidence indicates that the anti-mouse p75NTR will be effective for use as an immunotoxin.

Related Products: 192-IgG-SAP (Cat. #IT-01), mu p75-SAP (Cat. #IT-16), Antibody to NGFR (Cat. #AB-N02)

Kitahama K, Nagatsu I, Geffard M, Maeda T (2000) Distribution of dopamine-immunoreactive fibers in the rat brainstem. . J Chem Neuroanat 18(1-2):1-9. doi: 10.1016/s0891-0618(99)00047-2 PMID: 10708914

Related Products: Dopamine Mouse Monoclonal, Conjugated (Cat. #AB-T11)

Reyes-Reyna SM, Krolick KA (2000) Chemokine production by rat myocytes exposed to interferon-gamma. Clin Immunol 94(2):105-113. doi: 10.1006/clim.1999.4828 PMID: 10637095

Related Products: Nicotinic Acetylcholine Receptor alpha 1 (mAb 35) Rat Monoclonal (Cat. #AB-N36)

Steggerda SM, Black BE, Paschal BM (2000) Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran. Mol Biol Cell 11(2):703-719. doi: 10.1091/mbc.11.2.703 PMID: 10679025

Related Products: Antibody to NTF2 (5A3) (Cat. #AB-V66), Antibody to NTF2 (4F5) (Cat. #AB-V67), Antibody to NTF2 (5E8) (Cat. #AB-V68)

Fabbrini MS, Carpani D, Soria MR, Ceriotti A (2000) Cytosolic immunization allows the expression of preATF-saporin chimeric toxin in eukaryotic cells. FASEB J 14(2):391-398. doi: 10.1096/fasebj.14.2.391 PMID: 10657995

Related Products: Saporin Goat Polyclonal (Cat. #AB-15)

Provencio I, Rodriguez IR, Jiang G, Hayes WP, Moreira EF, Rollag MD (2000) A novel human opsin in the inner retina. J Neurosci 20(2):600-605. doi: 10.1523/JNEUROSCI.20-02-00600.2000 PMID: 10632589

Summary: Provencio and colleagues found that melanopsin is also present in mouse retina, specifically in ganglion cells, and that it mediates non-visual photoreceptive tasks.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38), Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Barker-Gibb ML, Einheber S, Milner TA (1999) Hippocampal Trk-A receptors are compartmentalized within axonal transport vesicle sites and present in select astrocytes. Neuroscience 1999 Abstracts 803.6. Society for Neuroscience, Miami, FL. PMID: 0

Summary: Trk-A is a high affinity transmembrane tyrosine kinase receptor which is activated by its ligand, nerve growth factor (NGF), to mediate cell survival and the retrograde transport of NGF by septohippocampal cholinergic neurons. To determine its subcellularlocalization, antibodies to the extracellular domain of the Trk-A receptor (Chemicon; Upstate) were localized immunocytochemically in rat dentate gyrus by light & electron microscopy (LM; EM). These antibodies recognized two bands migrating at -125 and 140 kD on immunoblots of septal and hippocampal lysates. Consistent with previous LM reports, Trk-A-immuno- reactivity (-I) with both antibodies was found in fine, varicose fibers, primarily in the hilus. However, the Chemicon antibody also labeled fibers in the granule and molecular layers (ML) as well as numerous astrocytic profiles in the external and medial zones of the ML and the superficial blade of the hilus. EM analysis of the dentate gyrus using the Chemicon antibody revealed that Trk-A-I was in axons, axon terminals and astrocytes. Trk-A-I in axons was not homogeneous, but was concentrated in patches near regions where vesicles accumulated. Trk-A-I in terminals was affiliated with clusters of small synaptic vesicles frequently located away from synaptic specializations. In both axons and axon terminals, the clusters of Trk-A-labeled vesicles often were adjacent to somatic and dendritic profiles. Trk-A-labeled astrocytes in the hilus regularly closely apposed terminals presynaptic to dendritic spines. The findings support evidence that hippocampal Trk-A receptors are compartmentalized within transport vesicle sites. Also, they suggest that astrocytes play a role in the uptake and retrograde transport of NGF by septohippocampal cholinergic neurons to support their survival. (Support: MH42834; DA 08259)

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)