antibodies

Alencar JL, Lobysheva I, Geffard M, Sarr M, Schott C, Schini-Kerth VB, Nepveu F, Stoclet JC, Muller B (2003) Role of S-nitrosation of cysteine residues in long-lasting inhibitory effect of nitric oxide on arterial tone. Mol Pharmacol 63(5):1148-1158. doi: 10.1124/mol.63.5.1148 PMID: 12695543

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Panda S, Sato TK, Castrucci AM, Rollag MD, DeGrip WJ, Hogenesch JB, Provencio I, Kay SA (2002) Melanopsin (Opn4) requirement for normal light-induced circadian phase shifting. Science 298(5601):2213-2216. doi: 10.1126/science.1076848 PMID: 12481141

Objective: To investigate the role of melanopsin in circadian photoentrainment in mammals.

Summary: The study focused on understanding how melanopsin, an opsin-based photopigment, affects the circadian rhythms in mammals. By generating melanopsin-null mice and observing their response to light-induced circadian phase shifting, the research demonstrated that while these mice maintain some entrainment to light/dark cycles, their phase resetting in response to monochromatic light is severely attenuated. This highlights melanopsin’s critical role in circadian photoentrainment, alongside other mechanisms that contribute to this process.

Usage: Anti-Melanopsin (AB-N38) was incubated with tissue for 24 hr at 4°C at a 1:2,500 dilution in a TBS incubating buffer containing 1% bovine serum albumin, 0.25% carrageenan lambda and 0.003% Triton X-100.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38), Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Hartig W, Varga C, Kacza J, Grosche J, Seeger J, Luiten PG, Brauer K, Harkany T (2002) In vivo labeling of rabbit cholinergic basal forebrain neurons with fluorochromated antibodies. NeuroReport 13(11):1395-1398. doi: 10.1097/00001756-200208070-00009 PMID: 12167760

Summary: To investigate in vivo labeling of p75 low-affinity neurotrophin receptor the authors conjugated Cy3 to ME20.4 (Cat. #AB-N07) and performed either unilateral or bilateral icv injections in rabbits. The antibody labeled only cholinergic neurons demonstrating its potential as a p75 marker.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Lappi D, Kohls M, Majer K, Russell B, Blakely R Richerson G (2002) Targeting serotonin re-uptake transporter (SERT) -expressing cells with a monoclonal antibody to an epitope from the extracellular domain of SERT: Results with a saporin conjugate. FENS 2002 Abstracts 049.7. Federation of European Neuroscience Societies, Paris, France. PMID: 0

Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09), Anti-SERT-SAP (Cat. #IT-23)

Lembo PM, Grazzini E, Groblewski T, O’Donnell D, Roy MO, Zhang J, Hoffert C, Cao J, Schmidt R, Pelletier M, Labarre M, Gosselin M, Fortin Y, Banville D, Shen SH, Strom P, Payza K, Dray A, Walker P, Ahmad S (2002) Proenkephalin A gene products activate a new family of sensory neuron-specific GPCRs. Nat Neurosci 5(3):201-209. doi: 10.1038/nn815 PMID: 11850634

Related Products: BAM22P Rabbit Polyclonal (Cat. #AB-12)

Provencio I, Rollag MD, Castrucci AM (2002) Photoreceptive net in the mammalian retina. Nature 415:493. doi: 10.1038/415493a PMID: 11823848

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Vulchanova L, Olson TH, Stone LS, Riedl MS, Elde R, Honda CN (2001) Cytotoxic targeting of isolectin IB4-binding sensory neurons. Neuroscience 108(1):143-155. doi: 10.1016/s0306-4522(01)00377-3 PMID: 11738138

Summary: Vulchanova et al. examine the role of IB4-binding neurons in nociception. IB4-SAP (Cat. #IT-10) was injected into rats (2 µg in left sciatic nerve). The resulting ablation of IB4-binding neurons provides evidence for their role in nociceptive processing and demonstrates a rapid compensatory response to signalling of acute pain.

Related Products: IB4-SAP (Cat. #IT-10), Saporin Goat Polyclonal (Cat. #AB-15), Saporin Goat Polyclonal, HRP-labeled (Cat. #AB-15HRP)

Kohls MD, Majer KA, Russell BJ, Han Q, Blakely RD, Lappi DA (2001) A monoclonal antibody to an extracellular domain of the serotonin transporter: Characterization and targeting properties. Neuroscience 2001 Abstracts 814.9. Society for Neuroscience, San Diego, CA. PMID: 0

Summary: Using a peptide corresponding to a sequence from an extracellular domain of the rat serotonin re-uptake transporter (SERT), we have created a monoclonal antibody that recognizes the molecule on the cell surface. In FACS analysis, the antibody is able to recognize SERT-expressing human platelets and rat basophilic RBL-2H3 cells. Recognition is inhibited by the peptide immunogen. In western blotting of SERT-transfected cells, the antibody recognizes an approximately 95 kDa band corresponding to SERT, and shows no such recognition in the parent cell line. We have created an immunotoxin with this antibody by conjugation to the ribosome-inactivating protein saporin. This immunotoxin is 50-fold more cytotoxic than saporin to RBL-2H3 cells, according to ED50. The ED50 for the immunotoxin is 10.6 nM, whereas the ED50 for non-conjugated saporin is 477 nM. At 100 nM, the immunotoxin eliminates 72% of cells, whereas saporin alone has no significant difference on cells from control (addition of vehicle). These data demonstrate that antibody binding to SERT results in internalization, a situation we have also seen with the dopamine transporter (R.G. Wiley, M.B. Harrison, A.I. Levey and D.A. Lappi, submitted). Antibodies for the targeting and delivery of molecules to the interior of cells can be created through the use of peptide immunogens derived from the sequences of the extracellular domains.

Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09)

Waite JJ, Chen AD (2001) Differential changes in rat cholinergic parameters subsequent to immunotoxic lesion of the basal forebrain nuclei. Brain Res 918:113-120. doi: 10.1016/s0006-8993(01)02968-7 PMID: 11684049

Summary: 192-Saporin (Cat. #IT-01) is used extensively to eliminate the cholinergic neurons of the basal forebrain in rats. Waite and Chen compare the degree of loss between 192-Saporin (6 or 8.2 µg in 10 µl into left lateral ventricle) and control (Saporin, 1.82 µg into left lateral ventricle; Cat. #PR-01) using three methods: Assay of post mortem choline acetyltransferase activity, in vivo microdialysis of extracellular acetylcholine (ACh), and in vivo assessment of the rate of ACh synthesis. The infusion of saporin alone had no effect. After fifteen weeks, the authors report compensation of cholinergic activity in lesioned animals occurs in the hippocampus, but not in the frontal cortex as determined by measurement of the rate of ACh synthesis.

Related Products: 192-IgG-SAP (Cat. #IT-01), Saporin Goat Polyclonal (Cat. #AB-15), Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP), Saporin (Cat. #PR-01)

Sinakevitch I, Farris SM, Strausfeld NJ (2001) Taurine-, aspartate- and glutamate-like immunoreactivity identifies chemically distinct subdivisions of Kenyon cells in the cockroach mushroom body. J Comp Neurol 439(3):352-367. doi: 10.1002/cne.1355 PMID: 11596059

Related Products: L-Glutamate Rabbit Polyclonal, Conjugated (Cat. #AB-T08), L-Glutamate Mouse Monoclonal, Conjugated (Cat. #AB-T12), D-Glutamic Acid (D-Glutamate) Rabbit Polyclonal, Conjugated (Cat. #AB-T045)