antibodies

Boisvert EM, Engle SJ, Hallowell SE, Liu P, Wang ZW, Li XJ (2015) The specification and maturation of nociceptive neurons from human embryonic stem cells. Sci Rep 5:16821. doi: 10.1038/srep16821 PMID: 26581770

Usage: Immunocytochemistry 1:200

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Buhr E, Yue W, Ren X, Jiang Z, Liao H, Mei X, Vemaraju S, Nguyen M, Reed R, Lang R, Yau K, Van Gelder R (2015) Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea. Proc Natl Acad Sci U S A 112:13093-13098. doi: 10.1073/pnas.1516259112 PMID: 26392540

Summary: Circadian clocks are found in most mammalian tissues. These clocks are synchronized by the suprachiasmatic nuclei (SCN) in the brain. The local clock found in the retina does not require rods, cones, intrinsically photosensitive retinal ganglion cells, or the SCN. In order to determine what photopigments are responsible for local retinal photoentrainment, the authors used a candidate gene approach. For immunohistochemical studies on flat mount retinas they used a melanopsin antibody (Cat. #AB-N38) at a 1:1000 dilution. The data indicate that OPN5, also known as neuropsin, has a light-sensing function and is involved in retinal photoentrainment.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Werner A, Iwasaki S, McGourty CA, Medina-Ruiz S, Teerikorpi N, Fedrigo I, Ingolia NT, Rape M (2015) Cell-fate determination by ubiquitin-dependent regulation of translation. Nature 525(7570):523-527. doi: 10.1038/nature14978 PMID: 26399832

Usage: Immunofluorescence 1:100

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Riazifar H, Sun G, Wang X, Rupp A, Vemaraju S, Ross-Cisneros F, Lang R, Sadun A, Hattar S, Guan M, Huang T (2015) Phenotypic and functional characterization of Bst+/- mouse retina. Dis Model Mech 8:969-976. doi: 10.1242/dmm.018176 PMID: 26035379

Summary: The belly spot and tail mutant mouse strain was first reported on in 1976. Among other phenotypic changes, it carries ocular mutations including retinal colobomas, reduced retinal ganglion cells (RGCs), and axon misrouting. In order to assess the use of this strain as a murine model for stem cell therapies of retinal degenerative diseases the authors performed a number of characterization experiments including electron microscopy, immunohistochemistry, testing of circadian rhythms, and morphological studies. Some of the immunohistochemistry was done using Anti-Melanopsin (Cat. #AB-N38) at a 1:5000 dilution.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Gibon J, Buckley S, Unsain N, Kaartinen V, Séguéla P, Barker P (2015) proBDNF and p75NTR control excitability and persistent firing of cortical pyramidal neurons. J Neurosci 35:9741-9753. doi: 10.1523/JNEUROSCI.4655-14.2015 PMID: 26134656

Summary: Principal neurons in the entorhinal cortex (EC) display persistent firing (PF) during working-memory tasks. Much of the communication between the hippocampus and the neocortex passes through the EC, and the EC also receives some cholinergic input from the medial septum and diagonal band of Broca. In this work the authors investigated the role of pro-brain-derived neurotrophic factor (proBDNF) and the p75 receptor in excitability and PF in the EC. The authors propose the proBDNF/p75 system as a regulator for pyramidal neuron excitability and PF in the EC, preventing runaway activity. Some of the western blot and current-clamp data was generated using Anti-p75 (Cat. #AB-N01; no concentration information provided).

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

El-Danaf R, Huberman A (2015) Characteristic patterns of dendritic remodeling in early-stage glaucoma: evidence from genetically identified retinal ganglion cell types. J Neurosci 35:2329-2343. doi: 10.1523/JNEUROSCI.1419-14.2015 PMID: 25673829

Summary: The loss of retinal ganglion cells (RGC) is the second-most common cause of blindness worldwide. Using several mouse transgenic cell lines, the authors investigated the changes that occur on the establishment of elevated ocular pressure. Anti-melanopsin (Cat. #AB-N39) at 1:1000 was used to illuminate the morphology of the M1 intrinsically photosensitive RGC.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Ma R, Latif R, Davies TF (2015) Human embryonic stem cells form functional thyroid follicles. Thyroid doi: 10.1089/thy.2014.0537 PMID: 25585054

Summary: Lysates were prepared from single- and double-transfected cells by treating with 1 ·RIPA buffer for 20 min on ice. The samples were then centrifuged and treated with 5 · sample buffer at 100°C for 5 min and loaded on a 4–15% SDS-PAGE gel. Western blotting was performed according to a standard protocol.

Related Products: TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)

Mao C, Li H, Zhang Z, Kiyama T, Panda S, Hattar S, Ribelayga C, Mills S, Wang S (2014) T-box transcription regulator Tbr2 is essential for the formation and maintenance of Opn4/melanopsin-expressing intrinsically photosensitive retinal ganglion cells. J Neurosci 34:13083-13095. doi: 10.1523/JNEUROSCI.1027-14.2014 PMID: 25253855

Summary: Opsin 4/melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) are responsible for controlling non-image-forming visual functions in the retina. The findings show that opsin 4 is only expressed in Tbr2-positive ipRGCs, no ipRGCs are found if Tbr2 is deleted before RGC specialization, and most ipRGCs are eliminated when Tbr2 is deleted from established ipRGCs. An antibody against melanopsin (Cat. #AB-N39) was used at a 1:1000 dilution for immunohistochemical analyses.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Luo W, Lin B, Wang Y, Zhong J, O’Meally R, Cole R, Pandey A, Levchenko A, Semenza G (2014) PHD3-mediated prolyl hydroxylation of nonmuscle actin impairs polymerization and cell motility. Mol Biol Cell 25:2788-2796. doi: 10.1091/mbc.E14-02-0775 PMID: 25079693

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Alvares T, Revill A, Huxtable A, Lorenz C, Funk G (2014) P2Y1 receptor-mediated potentiation of inspiratory motor output in neonatal rat in vitro. J Physiol 592:3089-3111. doi: 10.1113/jphysiol.2013.268136 PMID: 24879869

Summary: P2YR’s are metabotropic purinergic receptors found in some parts of the CNS. A subtype of this receptor excites rhythm generating networks in the preBötzinger complex. In order to better understand the role of these receptors in modulation of motor output the authors used brainstem-spinal cord and medullary slice preparations from neonatal rats to investigate P2Y1R signaling on specific neurons that innervate diaphragm and airway muscles. Anti-NK1r (Cat. #AB-N33AP) at a 1:1000 dilution was used during the immunohistochemistry. The data suggest that loss of purinergic modulation contributes to motoneuron excitability.

Related Products: NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)