antibodies

Khan N, Muralidharan A, Smith MT (2017) Attenuation of the infiltration of angiotensin ii expressing CD3+ T-cells and the modulation of nerve growth factor in lumbar dorsal root ganglia – a possible mechanism underpinning analgesia produced by EMA300, an Angiotensin II type 2 (AT2) receptor antagonist. Front Mol Neurosci 10:389. doi: 10.3389/fnmol.2017.00389 PMID: 29200998

Objective: To investigate the cellular and molecular mechanism of action of selective small molecule angiotensin II type 2 (AT2) receptor antagonists in the alleviation of peripheral neuropathic pain.

Summary: The analgesic effect of EMA300 in CCI-rats involves multimodal actions that appear to be mediated at least in part by a significant reduction in the otherwise increased expression levels of Ang II as well as the number of Ang II-expressing CD3+ T-cells in the ipsilateral lumbar DRGs of CCI-rats.

Usage: Immunocytochemistry; specifically labeled HEK cells expressing the AT2 but not the AT1 receptor or the non-transfected HEK-cells.

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Lovatt M, Yam GH-F, Peh GS, Colman A, Dunn NR, Mehta JS (2018) Directed differentiation of periocular mesenchyme from human embryonic stem cells. Differentiation 99:62-69. doi: 10.1016/j.diff.2017.11.003 PMID: 29239730

Objective: Pluripotent stem cells are attractive sources of cells for regenerative medicine, because large numbers of therapeutically useful cells can be generated. However, a detailed understanding of how to differentiate clinically relevant cell types from stem cells is fundamentally required.

Summary: Identification of cells resembling periocular mesenchyme (POM) cells in the adult cornea, located in a niche between the trabecular meshwork and peripheral endothelium. The generation and expansion of POM is an important step in the generation of a number of cells types that could prove to be clinically useful for a number of diseases of the cornea.

Usage: 1:200 for flow cytometry and immunofluorescence.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Wang C, Zhang C, Zhou F, Gao L, Wang Y, Wang C, Zhang Y (2018) Angiotensin II induces monocyte chemoattractant protein-1 expression by increasing reactive oxygen species-mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts. Mol Med Rep 17:1166-1172. doi: 10.3892/mmr.2017.7971 PMID: 29115506

Objective: To investigate the effect of angiotensin II (Ang II) on monocyte chemoattractant protein‑1 (MCP‑1) expression and the underlying mechanism in osteoblasts.

Summary: These data indicate that Ang II-enhanced ROS production and activated NF‑κB signaling via AT1R, thus upregulating MCP‑1 expression in osteoblasts.

Usage: Western Blot; the membranes were blocked with 5% fat‑free milk and probed with anti‑AT1R (1:600).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Fraser M, Fortier M, Foucher D, Roumier P, Brousseau P, Fournier M, Surette C, Vaillancourt C (2018) Exposure to low environmental concentrations of manganese, lead, and cadmium alters the serotonin system of blue mussels. Environ Toxicol Chem 37:192-200. doi: 10.1002/etc.3942 PMID: 28796292

Objective: To determine the effects of exposure to Mn, Pb, or Cd on serotonin levels, monoamine oxidase (MAO) activity, and serotonin transporter (SERT) levels in the blue mussel Mytilus edulis.

Summary: The results clearly indicate that low environmental concentrations of Mn, Pb, and Cd affect the serotonin system in blue mussels.

Usage: Membrane was exposed to anti-SERT primary antibody diluted 1:1000 in 5% PBS skimmed milk for 1.5 h at room temperature, then rinsed twice in deionized water, washed 3 times for 5 min in PBS, and rinsed 4 times in deionized water.

Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)

Lindborg JA, Mack M, Zigmond RE (2017) Neutrophils are critical for myelin removal in a peripheral nerve injury model of Wallerian degeneration. J Neurosci 37(43):10258-10277. doi: 10.1523/JNEUROSCI.2085-17.2017 PMID: 28912156

Objective: To characterize Wallerian degeneration (WD) in male Ccr2−/−mice and identify a compensatory mechanism of WD that is facilitated primarily by neutrophils.

Summary: The data have shown that neutrophils play an impressive role in myelin removal during WD. Neutrophil-specific depletion severely abates nerve debris clearance not only in Ccr2−/− mice, but in WT mice as well. The findings show a novel role for neutrophils that better elucidates the process of WD in the peripheral nervous system.

Usage: immunohistochemistry (1:400)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Cai S, Tsui YP, Tam KW, Shea GK, Chang RS, Ao Q, Shum DK, Chan YS (2017) Directed differentiation of human bone marrow stromal cells to fate-committed schwann cells. Stem Cell Reports 9(4):1097-1108. doi: 10.1016/j.stemcr.2017.08.004 PMID: 28890164

Objective: To create a protocol for in vitro derivation of fate-committed Schwann cells (SCs) from human bone marrow stromal cells (BMSCs).

Summary: This study demonstrates that the human bone marrow harbors neuro-ectodermal progenitors that can be enriched, expanded, and directed to differentiate into functionally mature, fate-committed SCs.

Usage: IF (1:500) and WB (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Li G, Liu J, Wang Y, Yang K, Zhao M, Xiao Y, Wen X, Liu L (2017) LNGFR targets the Wnt/β-catenin pathway and promotes the osteogenic differentiation in rat ectomesenchymal stem cells. Sci Rep 7(1):11021. doi: 10.1038/s41598-017-11555-9 PMID: 28887537

Objective: To demonstrate that nuclear β-catenin was upregulated during ectomesenchymal stem cells (EMSC) osteogenic differentiation.

Summary: The data reveal that the low affinity nerve growth factor receptor (LNGFR) targets the Wnt/β-catenin pathway and positively regulates EMSC osteogenic differentiation, suggesting that Wnt/β-catenin pathway may be involved in the development of teeth and that the targeting Wnt/β-catenin pathway may have great potential for applications in dental tissue engineering regeneration.

Usage: Western blot

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Yu X, Liu Z, Hou R, Nie Y, Chen R (2017) Nerve growth factor and its receptors on onset and diagnosis of ovarian cancer. Oncol Lett 14(3):2864-2868. doi: 10.3892/ol.2017.6527 PMID: 28928825

Objective: To investigate the effect of nerve growth factor (NGF) and its receptors on the onset and diagnosis of ovarian cancer.

Summary: NGF and its receptor can contribute to the occurrence of ovarian cancer, and the onset condition of ovarian cancer can be diagnosed through the detection of high or low expression of NGF and its receptors.

Usage: ELISA, WB, IHC (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Wang G, Ma Z, Cao L, Yan G, Wang Y, Jin Y, Shen H, Zhang Y, Xu X, Chen X, Shen Z (2017) A novel method for obtaining highly enriched Schwann cell populations from mature monkey nerves based on in vitro pre‑degeneration. Mol Med Rep 16(5):6600-6607. doi: 10.3892/mmr.2017.7427 PMID: 28901508

Objective: To identify a novel technique that can efficiently isolate and purify Schwann cells (SCs) from mature monkey nerves based on in vitro pre-degeneration.

Summary: Based on their observations, the migration rate of SCs out of the nerve explants appeared to reach its maximum after 14 days. However, the level of fibroblast proliferation was also significantly increased, which reduced the purity of the SCs. Therefore, the shorter 7-day pre-degeneration period may be optimal to maximize SC purity. These Schwann cells may be used to provide support for regenerating peripheral or central nerves.

Usage: Immunofluorescence (1:500) and flow cytometry

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Falade TE, Olude MA, Mustapha OA, Mbajiorgu EF, Ihunwo AO, Olopade JO, Oke BO (2017) Connective tissue, glial and neuronal expressions in testis of the African giant rat (Cricetomys gambianus). J Morphological Sciences 34(03):186-193. doi: 10.4322/jms.109117

Objective: To investigate the expression of connective tissue (Collagens I and III), glia and neuronal markers in the testis of the African giant rat.

Summary: The cyto-architecture of the testis was typical in the African giant rat. The connective tissue expressed in the juvenile and adult group, signaling of glial-like cells were seen in the perivascular region across the experimental groups. Immuno-localization of neuronal cells was seen in the interstitial spaces across all the groups, but with more expressions in the juvenile.

Usage: IHC (1:700)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)