Khan N, Muralidharan A, Smith MT (2017) Attenuation of the infiltration of angiotensin ii expressing CD3+ T-cells and the modulation of nerve growth factor in lumbar dorsal root ganglia – a possible mechanism underpinning analgesia produced by EMA300, an Angiotensin II type 2 (AT2) receptor antagonist. Front Mol Neurosci 10:389. doi: 10.3389/fnmol.2017.00389 PMID: 29200998
Objective: To investigate the cellular and molecular mechanism of action of selective small molecule angiotensin II type 2 (AT2) receptor antagonists in the alleviation of peripheral neuropathic pain.
Summary: The analgesic effect of EMA300 in CCI-rats involves multimodal actions that appear to be mediated at least in part by a significant reduction in the otherwise increased expression levels of Ang II as well as the number of Ang II-expressing CD3+ T-cells in the ipsilateral lumbar DRGs of CCI-rats.
Usage: Immunocytochemistry; specifically labeled HEK cells expressing the AT2 but not the AT1 receptor or the non-transfected HEK-cells.