Fraser M, Fortier M, Foucher D, Roumier P, Brousseau P, Fournier M, Surette C, Vaillancourt C (2018) Exposure to low environmental concentrations of manganese, lead, and cadmium alters the serotonin system of blue mussels. Environ Toxicol Chem 37:192-200. doi: 10.1002/etc.3942
Objective: To determine the effects of exposure to Mn, Pb, or Cd on serotonin levels, monoamine oxidase (MAO) activity, and serotonin transporter (SERT) levels in the blue mussel Mytilus edulis.
Summary: The results clearly indicate that low environmental concentrations of Mn, Pb, and Cd affect the serotonin system in blue mussels.
Usage: Membrane was exposed to anti-SERT primary antibody diluted 1:1000 in 5% PBS skimmed milk for 1.5 h at room temperature, then rinsed twice in deionized water, washed 3 times for 5 min in PBS, and rinsed 4 times in deionized water.