antibodies

Sánchez-Migallón MC, Valiente-Soriano FJ, Nadal-Nicolás FM, Di Pierdomenico J, Vidal-Sanz M, Agudo-Barriuso M (2018) Survival of melanopsin expressing retinal ganglion cells long term after optic nerve trauma in mice. Exp Eye Res 174:93-97. doi: 10.1016/j.exer.2018.05.029 PMID: 29856984

Summary: Melanopsin-positive retinal ganglion cells (RGCs) do not respond to axotomy in the same way than the rest of RGCs, and so while image-forming RGCs die in two exponential phases, non-image forming RGCs die only during the first quick phase.

Usage: Retinas were dissected as flat mounts and double-immunostained against melanopsin (1:5000).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Motohashi T, Kunisada T (2019) Direct conversion of mouse embryonic fibroblasts into neural crest cells. (eds. Turksen K). In: Skin Stem Cells. Methods in Molecular Biology. 1879:307-321. Humana Press, New York, NY. doi: 10.1007/7651_2018_145 PMID: 29797008

Objective: To describe methods for the direct conversion of mouse embryonic fibroblasts into neural crest cells (NCCs).

Summary: Sox10-IRES-Venus mouse fibroblasts were used for the conversion and isolation of converted NCCs as SOX10-positive cells.

Usage: Immunostaining for Flow Cytometry

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Sun X, Zhu Y, Yin HY, Guo ZY, Xu F, Xiao B, Jiang WL, Guo WM, Meng HY, Lu SB, Wang Y, Peng J (2018) Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function. Stem Cell Res Ther 9:133. doi: 10.1186/s13287-018-0884-3 PMID: 29751848

Objective: To improve the traditional methods for inducing the differentiation of mesenchymal stromal cells (MSCs) into Schwann cell-like cells (SCLCs).

Summary: Results indicated that the intermittent induction method is more efficient than traditional methods for inducing adipose-derived stem cells to differentiate into SCLCs.

Usage: immunofluorescence (1:500)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Lynch TJ, Anderson PJ, Rotti PG, Tyler SR, Crooke AK, Choi SH, Montoro DT, Silverman CL, Shahin W, Zhao R, Jensen-Cody CW, Adamcakova-Dodd A, Evans TIA, Xie W, Zhang Y, Mou H, Herring BP, Thorne PS, Rajagopal J, Yeaman C, Parekh KR, Engelhardt JF (2018) Submucosal gland myoepithelial cells are reserve stem cells that can regenerate mouse tracheal epithelium. Cell Stem Cell 22(5):653-667.e5. doi: 10.1016/j.stem.2018.03.017 PMID: 29656941

Objective: To investigate whether a lineage relationship exists between two stem cell compartments that contribute to airway repair – basal cells in the surface airway epithelium (SAE) and an unknown submucosal gland (SMG) celltype.

Summary: Using lineage tracing of glandular myoepithelial cells (MECs), they demonstrate that MECs can give rise to seven cell types of the SAE and SMGs following severe airway injury. MECs progressively adopted a basal cell phenotype on the SAE and established asting progenitors capable of further regeneration following reinjury. MECs activate Wnt-regulated transcription factors (Lef-1/TCF7) following injury and Lef-1 induction in cultured MECs promoted transition to a basal cell phenotype. Surprisingly, dose-dependent MEC conditional activation of Lef-1in vivo promoted self-limited airway regeneration in the absence of injury.

Usage: immunofluorescence

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Tata A, Kobayashi Y, Chow RD, Tran J, Desai A, Massri AJ, McCord TJ, Gunn MD, Tata PR (2018) Myoepithelial cells of submucosal glands can function as reserve stem cells to regenerate airways after injury. Cell Stem Cell 22:668-683. doi: 10.1016/j.stem.2018.03.018 PMID: 29656943

Objective: To show that myoepithelial cells (MECs) of the submucosal glands (SMGs) proliferate and migrate to repopulate the airway surface epithelium (SE) in multiple injury models.

Summary: SMG-like cells appear on the SE of extra-and intra-lobular airways of large animal lungs following severe injury. The transcription factor SOX9 is necessary for MEC plasticity in airway regeneration.

Usage: Immunostaining

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Ogawa T, Li Y, Lua I, Hartner A, Asahina K (2018) Isolation of a unique hepatic stellate cell population expressing integrin α8 from embryonic mouse livers. 247(6):867-881. doi: 10.1002/dvdy.24634 PMID: 29665133

Objective: The aim of this study is to identify specific markers for embryonic Hepatic stellate cells (HSCs).

Summary: ITGA8 is a specific cell surface marker of MC-derived HSCs and perivascular mesenchymal cells in the developing liver. Our data suggest that ITGA8+ mesenchymal cells maintain the phenotype of hepatoblast in liver development.

Usage: Immunofluorescence (1:1000)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Bassil M, Atat AA, Ibragim M (2018) Detection of oxidative stress in buccal cells using iSWAB tubes. J Mol Biomark Diagn 9(2):1-6. doi: 10.4172/2155-9929.1000387 PMID: 909090

Objective: To investigate the oxidative profile of iSWAB-Mawi protein tubes by examining their ability to detect the protein oxidation biomarkers: nitrotyrosine and S-nitrosocysteine in buccal cells.

Summary: Results showed the presence of S-nitrosocysteine and nitrotyrosine in buccal cells reflecting the ability of iSWAB-Mawi protein tubes to detect those protein oxidation biomarkers.

Usage: Western Blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Wong JCY, Smyllie NJ, Banks GT, Pothecary CA, Barnard AR, Maywood ES, Jagannath A, Hughes S, van der Horst GTJ, MacLaren RE, Hankins MW, Hastings MH, Nolan PM, Foster RG, Peirson SN (2018) Differential roles for cryptochromes in the mammalian retinal clock. FASEB J 32:4302-4314. doi: 10.1096/fj.201701165RR PMID: 29561690

Objective: To determine roles of cryptochromes (CRY) in the retinal clock.

Summary: Data suggest that CRY1 is an essential component of the mammalian retinal clock, whereas CRY2 has a more limited role.

Usage: Immunohistochemistry 1:2500.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Morgan BJ, Schrimpf N, Rothman M, Mitzey A, Brownfield MS, Speth RC, Dopp JM (2018) Effect of chronic intermittent hypoxia on angiotensin II receptors in the central nervous system. Clin Exp Hypertens 41:1-7. doi: 10.1080/10641963.2018.1451536 PMID: 29561178

Objective: To quantify the effects of chronic intermittent hypoxia (CIH) on AT1R- and AT2R-like immunoreactivity in the rostroventrolateral medulla (RVLM) and paraventricular nucleus of the hypothalamus (PVN), central regions that are important components of the extended chemoreflex pathway.

Summary: Exposure to CIH of at least 5 days duration augmented AT1R-like immunoreactivity in RVLM. In contrast, CIH exposure did not affect AT2R-like reactivity in RVLM or that of either Ang II receptor subtype in PVN.

Usage: Immunoreactivity, immunostaining. For immunoreactivity assays, antibodies were diluted in the blocking solution with 0.05% sodium azide at the following concentrations: AT-1R, (1:1600) and AT-2R, (1:5000).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Ing-Esteves S, Kostadinov D, Marocha J, Sing AD, Joseph KS, Laboulaye MA, Sanes JR, Lefebvre JL (2018) Combinatorial effects of alpha- and gamma-protocadherins on neuronal survival and dendritic self-avoidance. J Neurosci 38:2713-2729. doi: 10.1523/JNEUROSCI.3035-17.2018 PMID: 29439167

Objective: The clustered protocadherins (Pcdhs) comprise 58 cadherin-related proteins encoded by three tandemly arrayed gene clusters, Pcdh- , Pcdh- , and Pcdh- (Pcdha, Pcdhb, and Pcdhg, respectively). This study sought to determine roles of Pcdhas and Pcdhgs in the retina and cerebellum from mice (both sexes) lacking one or both clusters.

Summary: Study examined two regions of the CNS, the retina and cerebellum and showed that the 14 -Pcdhs and 22 -Pcdhs act synergistically to mediate neuronal survival and dendrite patterning. In retina, Pcdhgs are essential for survival of inner retinal neurons and dendritic self-avoidance of starburst amacrine cells, whereas Pcdhas are dispensable for both processes.

Usage: Anti-Melanopsin (1:5000) used to quantify two mutually exclusive RGC cell types, the Brn3a RGCs and the melanopsin-positive intrinsically photo- sensitive RGCs (Mel ipRGCs).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)