antibodies

Micakovic T, Papagiannarou S, Clark E, Kuzay Y, Abramovic K, Peters J, Sticht C, Volk N, Fleming T, Nawroth P, Hammes HP, Alenina N, Grone HJ, Hoffmann SC (2018) The angiotensin II type 2 receptors protect renal tubule mitochondria in early stages of diabetes mellitus. Kidney Int 94:937-950. doi: 10.1016/j.kint.2018.06.006 PMID: 30190172

Objective: To investigate the role of the angiotensin II type 2 receptor (AT-2R) at the early stages of diabetic nephropathy.

Summary: Targeted AT-2R overexpression in renal cells may open new avenues to develop novel types of drugs preventing diabetic nephropathy.

Usage: Histology, immunohistochemistry; 1:100

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Donnelly CR, Gabreski NA, Suh EB, Chowdhury M, Pierchala BA (2018) Non-canonical Ret signaling augments p75-mediated cell death in developing sympathetic neurons. J Cell Biol 217:327. doi: 10.1083/jcb.201703120 PMID: 30018091

Summary: The authors investigated the interactions of pro-survival and pro-apoptotic receptors in Programmed Cell Death (PCD) using the sympathetic nervous system as a model. They demonstrate that Ret, a receptor tyrosine kinase required for the survival of many neuronal populations, is restricted to a subset of degenerating neurons that rapidly undergo apoptosis.

Usage: Proximity ligation assays (1:500); Western blotting (1:1,000)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Tools to Study Programmed Cell Death

Zhang Z, Jiang SM, Ma YP, Dai PL, Wang YN, Zou GM, Gao HM, Yang Y, Li WG (2019) Expression of the intrarenal angiotensin receptor and the role of renin-angiotensin system inhibitors in IgA nephropathy. Mol Cell Biochem 453:103-110. doi: 10.1007/s11010-018-3435-4 PMID: 30159797

Objective: To investigate the role of intrarenal renin-angiotensin system (RAS) in the development of human kidney disease. Patients with IgA nephropathy (IgAN) were screened and their clinical characteristics analyzed.

Summary: Overexpression of AT1R on the mesangial cells of IgAN patients is associated with mesangial cell proliferation, glomerular segmental sclerosis, and crescent formation.

Usage: immunohistochemistry (1:500)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Jo A, Xu J, Deniz S, Cherian S, DeVries SH, Zhu Y (2018) Intersectional strategies for targeting amacrine and ganglion cell types in the mouse retina. Front Neural Circuits 12:66. doi: 10.3389/fncir.2018.00066 PMID: 30186122

Objective: To obtain unambiguous information about retinal processing.

Summary: Results establish a foundation for future application of intersectional strategies in the retina and retino-recipient regions.

Usage: Immunohistochemistry 1:1000.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Tao X, Li N, Liu F, Hu Y, Liu J, Zhang Y-M (2018) In vitro examination of microglia-neuron crosstalk with BV2 cells, and primary cultures of glia and hypothalamic neurons. Heliyon 4:e00730. doi: 10.1016/j.heliyon.2018.e00730 PMID: 30148218

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Kosykh A, Beilin A, Sukhinich K, Vorotelyak E (2018) Postnatal neural crest stem cells from hair follicle interact with nerve tissue in vitro and in vivo. Tissue Cell 54:94-104. doi: 10.1016/j.tice.2018.08.005 PMID: 30309515

Objective: To investigate the interaction between Neural crest stem cells that located in the postnatal hair follicle (HF-NCSC) and a nerve tissue (embryonic and adult).

Summary: HF-NCSC maintain their NC potency in the interaction with the embryonic central nerve system tissue. The migration capacity of the HF NCSC corresponds to the developmental stage but not a type of a nerve tissue present in the co-culture. In contrast, an adult brain interaction changes the HF-NCSC status and reduces the proliferation.

Usage: immunohistochemistry (1:50)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Higashi Y, Shimizu T, Yamamoto M, Tanaka K, Yawata T, Shimizu S, Zou S, Ueba T, Yuri K, Saito M (2018) Stimulation of brain nicotinic acetylcholine receptors activates adrenomedullary outflow via brain inducible NO synthase-mediated S-nitrosylation. Br J Pharmacol 175(19):3758-3772. doi: 10.1111/bph.14445 PMID: 30007012

Objective: To examine central mechanisms for the (±)-epibatidine-induced responses, focusing on brain NOS and NO-mediated mechanisms, soluble GC (sGC) and protein S-nitrosylation (a posttranslational modification of protein cysteine thiol groups), in urethane-anaesthetized (1.0 g·kg1, i.p.) male Wistar rats.

Summary: Stimulation of brain nAChRs can induce elevation of plasma catecholamines through brain iNOS-derived NO-mediated protein Snitrosylation in rats. Therefore, brain nAChRs (at least α4β2 subtype) and NO might be useful targets for alleviation of catecholamines overflow induced by smoking.

Usage: Immunohistochemistry (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Matamoros AJ (2018) Microtubule-mediated nerve regeneration: Knocking down the microtubule severing protein fidgetin augments nerve regeneration in vitro and in vivo. Drexel University Thesis.

Objective: To explore the effects of fidgetin knockdown in adult dorsal root ganglia both in vitro and in vivo.

Summary: In vitro: fidgetin knockdown in adult dorsal root ganglia neurons increases a specific domain of microtubule mass responsible for expanding microtubules, increases the length of axons on and off the inhibitory spots, and promotes cellular growth on and across the inhibitory spots. In vivo: fidgetin augments nerve regeneration in vivo, thus highlighting fidgetin as a novel therapeutic target to augment nerve regeneration

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kucharczyk M, Kurek A, Pomierny B, Detka J, Papp M, Tota K, Budziszewska B (2018) The reduced level of growth factors in an animal model of depression is accompanied by regulated necrosis in the frontal cortex but not in the hippocampus. Psychoneuroendocrinology 94:121-133. doi: 10.1016/j.psyneuen.2018.05.008 PMID: 29775875

Objective: To investigate if the different types of stress alter neuronal plasticity markers distinctively in the frontal cortex (FCx) and in the hippocampus (Hp).

Summary: The authors concluded that chronic stress during pregnancy can result in serious alterations in the functioning of the FCx of the progeny, facilitating the development of depressive behavior later in life. They also suggest that the altered energy metabolism may redirect pro-NGF/p75NTR/ATF2 signaling in the cortical neurons towards cellular death resembling regulated necrosis, rather than apoptosis.

Usage: Western blot (1:1000)

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Bhat SA, Sood A, Shukla R, Hanif K (2019) AT2R activation prevents microglia pro-inflammatory activation in a nox-dependent manner: Inhibition of PKC activation and p47(PHOX) phosphorylation by PP2A. Mol Neurobiol 56(4):3005-3023. doi: 10.1007/s12035-018-1272-9 PMID: 30076526

Objective: To investigate the involvement of AT2R in NADPH oxidase (NOX)-mediated microglia activation.

Summary: AT2R, via PP2A-mediated inhibition of PKC, prevents the NOX activation, ROS generation, and subsequent pro-inflammatory activation of microglia.

Usage: Immunoblotting: membranes were incubated with respective primary antibodies AT1R (1:1000), AT2R (1:500). Immunocytochemistry: AT1R (1:100), AT2R (1:100).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)