antibodies

Kosykh A, Beilin A, Sukhinich K, Vorotelyak E (2018) Postnatal neural crest stem cells from hair follicle interact with nerve tissue in vitro and in vivo. Tissue Cell 54:94-104. doi: 10.1016/j.tice.2018.08.005 PMID: 30309515

Objective: To investigate the interaction between Neural crest stem cells that located in the postnatal hair follicle (HF-NCSC) and a nerve tissue (embryonic and adult).

Summary: HF-NCSC maintain their NC potency in the interaction with the embryonic central nerve system tissue. The migration capacity of the HF NCSC corresponds to the developmental stage but not a type of a nerve tissue present in the co-culture. In contrast, an adult brain interaction changes the HF-NCSC status and reduces the proliferation.

Usage: immunohistochemistry (1:50)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Higashi Y, Shimizu T, Yamamoto M, Tanaka K, Yawata T, Shimizu S, Zou S, Ueba T, Yuri K, Saito M (2018) Stimulation of brain nicotinic acetylcholine receptors activates adrenomedullary outflow via brain inducible NO synthase-mediated S-nitrosylation. Br J Pharmacol 175(19):3758-3772. doi: 10.1111/bph.14445 PMID: 30007012

Objective: To examine central mechanisms for the (±)-epibatidine-induced responses, focusing on brain NOS and NO-mediated mechanisms, soluble GC (sGC) and protein S-nitrosylation (a posttranslational modification of protein cysteine thiol groups), in urethane-anaesthetized (1.0 g·kg1, i.p.) male Wistar rats.

Summary: Stimulation of brain nAChRs can induce elevation of plasma catecholamines through brain iNOS-derived NO-mediated protein Snitrosylation in rats. Therefore, brain nAChRs (at least α4β2 subtype) and NO might be useful targets for alleviation of catecholamines overflow induced by smoking.

Usage: Immunohistochemistry (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Matamoros AJ (2018) Microtubule-mediated nerve regeneration: Knocking down the microtubule severing protein fidgetin augments nerve regeneration in vitro and in vivo. Drexel University Thesis.

Objective: To explore the effects of fidgetin knockdown in adult dorsal root ganglia both in vitro and in vivo.

Summary: In vitro: fidgetin knockdown in adult dorsal root ganglia neurons increases a specific domain of microtubule mass responsible for expanding microtubules, increases the length of axons on and off the inhibitory spots, and promotes cellular growth on and across the inhibitory spots. In vivo: fidgetin augments nerve regeneration in vivo, thus highlighting fidgetin as a novel therapeutic target to augment nerve regeneration

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kucharczyk M, Kurek A, Pomierny B, Detka J, Papp M, Tota K, Budziszewska B (2018) The reduced level of growth factors in an animal model of depression is accompanied by regulated necrosis in the frontal cortex but not in the hippocampus. Psychoneuroendocrinology 94:121-133. doi: 10.1016/j.psyneuen.2018.05.008 PMID: 29775875

Objective: To investigate if the different types of stress alter neuronal plasticity markers distinctively in the frontal cortex (FCx) and in the hippocampus (Hp).

Summary: The authors concluded that chronic stress during pregnancy can result in serious alterations in the functioning of the FCx of the progeny, facilitating the development of depressive behavior later in life. They also suggest that the altered energy metabolism may redirect pro-NGF/p75NTR/ATF2 signaling in the cortical neurons towards cellular death resembling regulated necrosis, rather than apoptosis.

Usage: Western blot (1:1000)

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Bhat SA, Sood A, Shukla R, Hanif K (2019) AT2R activation prevents microglia pro-inflammatory activation in a nox-dependent manner: Inhibition of PKC activation and p47(PHOX) phosphorylation by PP2A. Mol Neurobiol 56(4):3005-3023. doi: 10.1007/s12035-018-1272-9 PMID: 30076526

Objective: To investigate the involvement of AT2R in NADPH oxidase (NOX)-mediated microglia activation.

Summary: AT2R, via PP2A-mediated inhibition of PKC, prevents the NOX activation, ROS generation, and subsequent pro-inflammatory activation of microglia.

Usage: Immunoblotting: membranes were incubated with respective primary antibodies AT1R (1:1000), AT2R (1:500). Immunocytochemistry: AT1R (1:100), AT2R (1:100).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Ueki Y, Shchepetkina V, Lefcort F (2018) Retina-specific loss of Ikbkap/Elp1 causes mitochondrial dysfunction that leads to selective retinal ganglion cell degeneration in a mouse model of familial dysautonomia. Dis Model Mech 11(7):dmm033746. doi: 10.1242/dmm.033746 PMID: 29929962

Objective: To determine the pathophysiological mechanisms that are triggered by the absence of IKAP (inhibitor of kappa B kinase complex-associated protein) in the retina.

Summary: The loss of IKAP caused progressive degeneration of retinal ganglion cells (RGCs) by 1 month of age. There was no loss of melanopsin+ intrinsically photosensitive RGCs at 18 months. RGCs were the only cell type that degenerated, with the survival of other retinal neurons unaffected.

Usage: Immunohistochemistry (IHC), RGC Counts, and H&E staining – Anti-melanopsin (1:5000).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Chiang JT, Badrealam KF, Shibu MA, Kuo CH, Huang CYA-Ohoo, Chen BC, Lin YM, Viswanadha VP, Kuo WW, Huang CY (2018) Eriobotrya japonica ameliorates cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats. Environ Toxicol 33:1113-1122. doi: 10.1002/tox.22589 PMID: 29974613

Objective: To evaluate the prospective efficacy of E. japonica leave extract (EJLE) against Angiotensin-II induced cardiac hypertrophy.

Summary: Supplementation of EJLE could rescue Ang-II induced cardiac hypertrophy.

Usage: Western blot, immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Zhu Y, Zhao J, Han Q, Wang Z, Wang Z, Dong X, Li J, Liu L, Shen X (2018) The effect and mechanism of chinese herbal formula Sini Tang in heart failure after myocardial infarction in rats. Evid Based Complement Alternat Med 2018:5629342. doi: 10.1155/2018/5629342 PMID: 30050591

Objective: To investigate the effectiveness and mechanism of theChinese herbal formula Sini Tang (SNT) in heart failure after myocardial infarction in rats.

Summary: The Chinese herbal formula SNT could improve left ventricular systolic function in heart failure after myocardial infarction.

Usage: Western blot.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Kim M, Vargas M, Harlan B, Killoy K, Ball L, Comte-Walters S, Gooz M, Yamamoto Y, Beckman J, Barbeito L, Pehar M (2018) Nitration and glycation turn mature NGF into a toxic factor for motor neurons: A role for p75. Antioxid Redox Signal 28:1587-1602. doi: 10.1089/ars.2016.6966 PMID: 28537420

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Sparks NR (2018) The embryotoxic effects of harm reduction tobacco products on osteoblasts developing from human embryonic stem cells. University of California Riverside Thesis.

Objective: To investigate the effect of maternal smoking in the development of embryonic skeleton and the molecular mechanisms involved in skeletal embryotoxicity induced by conventional and harm-reduction tobacco.

Summary: An in vitro model based on human embryonic stem cells was developed, that mimics the development of the human embryo; their differentiation into osteoblasts in vitro can be used to study chemical toxicity and the molecular mechanisms thereof. Tobacco-induced oxidative stress disrupts osteogenic differentiation in human embryonic stem cells.

Usage: Immunocytochemistry

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)