Angiotensin II receptor

Micakovic T, Papagiannarou S, Clark E, Kuzay Y, Abramovic K, Peters J, Sticht C, Volk N, Fleming T, Nawroth P, Hammes HP, Alenina N, Grone HJ, Hoffmann SC (2018) The angiotensin II type 2 receptors protect renal tubule mitochondria in early stages of diabetes mellitus. Kidney Int 94:937-950. doi: 10.1016/j.kint.2018.06.006 PMID: 30190172

Objective: To investigate the role of the angiotensin II type 2 receptor (AT-2R) at the early stages of diabetic nephropathy.

Summary: Targeted AT-2R overexpression in renal cells may open new avenues to develop novel types of drugs preventing diabetic nephropathy.

Usage: Histology, immunohistochemistry; 1:100

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Zhang Z, Jiang SM, Ma YP, Dai PL, Wang YN, Zou GM, Gao HM, Yang Y, Li WG (2019) Expression of the intrarenal angiotensin receptor and the role of renin-angiotensin system inhibitors in IgA nephropathy. Mol Cell Biochem 453:103-110. doi: 10.1007/s11010-018-3435-4 PMID: 30159797

Objective: To investigate the role of intrarenal renin-angiotensin system (RAS) in the development of human kidney disease. Patients with IgA nephropathy (IgAN) were screened and their clinical characteristics analyzed.

Summary: Overexpression of AT1R on the mesangial cells of IgAN patients is associated with mesangial cell proliferation, glomerular segmental sclerosis, and crescent formation.

Usage: immunohistochemistry (1:500)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Bhat SA, Sood A, Shukla R, Hanif K (2019) AT2R activation prevents microglia pro-inflammatory activation in a nox-dependent manner: Inhibition of PKC activation and p47(PHOX) phosphorylation by PP2A. Mol Neurobiol 56(4):3005-3023. doi: 10.1007/s12035-018-1272-9 PMID: 30076526

Objective: To investigate the involvement of AT2R in NADPH oxidase (NOX)-mediated microglia activation.

Summary: AT2R, via PP2A-mediated inhibition of PKC, prevents the NOX activation, ROS generation, and subsequent pro-inflammatory activation of microglia.

Usage: Immunoblotting: membranes were incubated with respective primary antibodies AT1R (1:1000), AT2R (1:500). Immunocytochemistry: AT1R (1:100), AT2R (1:100).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Chiang JT, Badrealam KF, Shibu MA, Kuo CH, Huang CYA-Ohoo, Chen BC, Lin YM, Viswanadha VP, Kuo WW, Huang CY (2018) Eriobotrya japonica ameliorates cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats. Environ Toxicol 33:1113-1122. doi: 10.1002/tox.22589 PMID: 29974613

Objective: To evaluate the prospective efficacy of E. japonica leave extract (EJLE) against Angiotensin-II induced cardiac hypertrophy.

Summary: Supplementation of EJLE could rescue Ang-II induced cardiac hypertrophy.

Usage: Western blot, immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Zhu Y, Zhao J, Han Q, Wang Z, Wang Z, Dong X, Li J, Liu L, Shen X (2018) The effect and mechanism of chinese herbal formula Sini Tang in heart failure after myocardial infarction in rats. Evid Based Complement Alternat Med 2018:5629342. doi: 10.1155/2018/5629342 PMID: 30050591

Objective: To investigate the effectiveness and mechanism of theChinese herbal formula Sini Tang (SNT) in heart failure after myocardial infarction in rats.

Summary: The Chinese herbal formula SNT could improve left ventricular systolic function in heart failure after myocardial infarction.

Usage: Western blot.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Morgan BJ, Schrimpf N, Rothman M, Mitzey A, Brownfield MS, Speth RC, Dopp JM (2018) Effect of chronic intermittent hypoxia on angiotensin II receptors in the central nervous system. Clin Exp Hypertens 41:1-7. doi: 10.1080/10641963.2018.1451536 PMID: 29561178

Objective: To quantify the effects of chronic intermittent hypoxia (CIH) on AT1R- and AT2R-like immunoreactivity in the rostroventrolateral medulla (RVLM) and paraventricular nucleus of the hypothalamus (PVN), central regions that are important components of the extended chemoreflex pathway.

Summary: Exposure to CIH of at least 5 days duration augmented AT1R-like immunoreactivity in RVLM. In contrast, CIH exposure did not affect AT2R-like reactivity in RVLM or that of either Ang II receptor subtype in PVN.

Usage: Immunoreactivity, immunostaining. For immunoreactivity assays, antibodies were diluted in the blocking solution with 0.05% sodium azide at the following concentrations: AT-1R, (1:1600) and AT-2R, (1:5000).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Maxwell GK, Szunyogova E, Shorrock HK, Gillingwater TH (2018) Developmental and degenerative cardiac defects in the Taiwanese mouse model of severe spinal muscular atrophy. J Anat 232:965-978. doi: 10.1111/joa.12793 PMID: 29473159

Objective: To investigate changes occurring in the heart, predominantly at pre- and early symptomatic ages, in the Taiwanese mouse model of severe Spinal muscular atrophy (SMA).

Summary: The findings support the requirement to develop systemic therapies for SMA capable of treating non-neuromuscular pathologies.

Usage: Immunohistochemistry, Western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Zhou H, Huang J, Zhu L, Cao Y (2018) Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues. Mol Med Rep 17:5184-5192. doi: 10.3892/mmr.2018.8473 PMID: 29393490

Objective: To determine whether erythropoietin (EPO) improves post‑resuscitation myocardial dysfunction and how it affects the renin‑angiotensin system.

Summary: The present study confirmed that EPO treatment is beneficial for protecting cardiac function post‑resuscitation, and the roles of EPO in alleviating post‑resuscitation myocardial dysfunction may potentially be associated with enhanced myocardial expression of AT2R.

Usage: Western blot analysis of AT-1R (1:500) in the myocardium

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Khan N, Muralidharan A, Smith MT (2017) Attenuation of the infiltration of angiotensin ii expressing CD3+ T-cells and the modulation of nerve growth factor in lumbar dorsal root ganglia – a possible mechanism underpinning analgesia produced by EMA300, an Angiotensin II type 2 (AT2) receptor antagonist. Front Mol Neurosci 10:389. doi: 10.3389/fnmol.2017.00389 PMID: 29200998

Objective: To investigate the cellular and molecular mechanism of action of selective small molecule angiotensin II type 2 (AT2) receptor antagonists in the alleviation of peripheral neuropathic pain.

Summary: The analgesic effect of EMA300 in CCI-rats involves multimodal actions that appear to be mediated at least in part by a significant reduction in the otherwise increased expression levels of Ang II as well as the number of Ang II-expressing CD3+ T-cells in the ipsilateral lumbar DRGs of CCI-rats.

Usage: Immunocytochemistry; specifically labeled HEK cells expressing the AT2 but not the AT1 receptor or the non-transfected HEK-cells.

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Wang C, Zhang C, Zhou F, Gao L, Wang Y, Wang C, Zhang Y (2018) Angiotensin II induces monocyte chemoattractant protein-1 expression by increasing reactive oxygen species-mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts. Mol Med Rep 17:1166-1172. doi: 10.3892/mmr.2017.7971 PMID: 29115506

Objective: To investigate the effect of angiotensin II (Ang II) on monocyte chemoattractant protein‑1 (MCP‑1) expression and the underlying mechanism in osteoblasts.

Summary: These data indicate that Ang II-enhanced ROS production and activated NF‑κB signaling via AT1R, thus upregulating MCP‑1 expression in osteoblasts.

Usage: Western Blot; the membranes were blocked with 5% fat‑free milk and probed with anti‑AT1R (1:600).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)