Morgan BJ, Schrimpf N, Rothman M, Mitzey A, Brownfield MS, Speth RC, Dopp JM (2018) Effect of chronic intermittent hypoxia on angiotensin II receptors in the central nervous system. Clin Exp Hypertens 41:1-7. doi: 10.1080/10641963.2018.1451536 PMID: 29561178
Objective: To quantify the effects of chronic intermittent hypoxia (CIH) on AT1R- and AT2R-like immunoreactivity in the rostroventrolateral medulla (RVLM) and paraventricular nucleus of the hypothalamus (PVN), central regions that are important components of the extended chemoreflex pathway.
Summary: Exposure to CIH of at least 5 days duration augmented AT1R-like immunoreactivity in RVLM. In contrast, CIH exposure did not affect AT2R-like reactivity in RVLM or that of either Ang II receptor subtype in PVN.
Usage: Immunoreactivity, immunostaining. For immunoreactivity assays, antibodies were diluted in the blocking solution with 0.05% sodium azide at the following concentrations: AT-1R, (1:1600) and AT-2R, (1:5000).