References

Wang C, Zhang C, Zhou F, Gao L, Wang Y, Wang C, Zhang Y (2018) Angiotensin II induces monocyte chemoattractant protein-1 expression by increasing reactive oxygen species-mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts. Mol Med Rep 17:1166-1172. doi: 10.3892/mmr.2017.7971 PMID: 29115506

Objective: To investigate the effect of angiotensin II (Ang II) on monocyte chemoattractant protein‑1 (MCP‑1) expression and the underlying mechanism in osteoblasts.

Summary: These data indicate that Ang II-enhanced ROS production and activated NF‑κB signaling via AT1R, thus upregulating MCP‑1 expression in osteoblasts.

Usage: Western Blot; the membranes were blocked with 5% fat‑free milk and probed with anti‑AT1R (1:600).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Lindborg JA, Mack M, Zigmond RE (2017) Neutrophils are critical for myelin removal in a peripheral nerve injury model of Wallerian degeneration. J Neurosci 37(43):10258-10277. doi: 10.1523/JNEUROSCI.2085-17.2017 PMID: 28912156

Objective: To characterize Wallerian degeneration (WD) in male Ccr2−/−mice and identify a compensatory mechanism of WD that is facilitated primarily by neutrophils.

Summary: The data have shown that neutrophils play an impressive role in myelin removal during WD. Neutrophil-specific depletion severely abates nerve debris clearance not only in Ccr2−/− mice, but in WT mice as well. The findings show a novel role for neutrophils that better elucidates the process of WD in the peripheral nervous system.

Usage: immunohistochemistry (1:400)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Llorente-ovejero A, Manuel I, Giralt MT, Rodríguez-puertas R (2017) Increase in cortical endocannabinoid signaling in a rat model of basal forebrain cholinergic dysfunction. Neuroscience 362:206-218.. doi: 10.1016/j.neuroscience.2017.08.008

Objective: To evaluate the eCB signaling in relation to the memory impairment induced in adult rats following a specific cholinergic lesion of the basal forebrain.

Summary: CB1 receptors present in presynaptic GABAergic terminals in the hippocampus are down regulated, but not those in cortical glutamatergic synapses.

Usage: 192-IgG-SAP was dissolved in aCSF under aseptic conditions to a final concentration of 130 ng/ml. aCSF or 192-IgG-SAP was bilaterally injected (1 ml/hemisphere) at a constant rate of 0.2 ml/min.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Leduc-Pessah H, Weilinger N, Fan C, Burma N, Thompson R, Trang T (2017) Site-specific regulation of P2X7 receptor function in microglia gates morphine analgesic tolerance. J Neurosci 37:10154-10172.. doi: 10.1523/JNEUROSCI.0852-17.2017

Summary: By selectively ablating microglia in the spinal cord using a Mac-1-SAP the authors demonstrate a causal role for microglia in the development, but not maintenance, of morphine tolerance in male rats.

Usage: Mac-1-SAP or unconjugated Saporin control (15 μg) was administered by intrathecal injection.

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06), Saporin (Cat. #PR-01)

Xiao H, Li M, Cai J, Li N, Zhou M, Wen P, Xie Z, Wang Q, Chang J, Zhang W (2017) Selective cholinergic depletion of pedunculopontine tegmental nucleus aggravates freezing of gait in parkinsonian rats. Neurosci Lett 659:92-98.. doi: 10.1016/j.neulet.2017.08.016

Summary: Many patients with advanced Parkinson’s disease suffer from gait and postural impairments. The authors used Anti-ChAT-SAP (Cat. #IT-42) to specifically lesion neurons in the Pedunculopontine Tegmental Nucleus (PPTg) to examine the impact on gait performance. Adult male rats received either unilateral PPTg cholinergic lesion or bilateral PPTg lesion, at a dose of 250 ng. The authors conclude that the cholinergic neurons of pedunculopontine tegmental nucleus play a vital role in the occurrence of gait freezing in Parkinson’s disease.

Related Products: Anti-ChAT-SAP (Cat. #IT-42)

Zielinski M, Gerashchenko D (2017) Sleep-inducing effect of substance P-cholera toxin A subunit in mice. Neurosci Lett 659:44-47.. doi: 10.1016/j.neulet.2017.08.066

Objective: To determine the effects of selectively activating SP-expressing brain cells on sleep regulation in mice.

Summary: ICV administration of SP-CTA produces increased amounts of NREM sleep but induces sleep fragmentation.

Usage: 1 mcg/mcl icv.

Related Products: SP-CTA (Cat. #IT-39)

Komatsu N, Mitsui K, Kusano-Arai O, Iwanari H, Hoshi K, Takato T, Abe T, Hamakubo T (2017) Enhancement of anti-Robo1 immunotoxin cytotoxicity to head and neck squamous cell carcinoma via photochemical internalization. Arch Can Res 5:157-163. doi: 10.21767/2254-6081.100157

Objective: To screen a monoclonal antibody to Robo1, an axon guidance receptor, for its suitability to target various cancers.

Summary: Conventional treatment exhibited an inadequate cytotoxic effect. With the addition of a photosensitizer and LED light illumination, the cytotoxic effect was remarkably improved.

Usage: Saporin-conjugated anti-Robo1 and Saporin-conjugated negative control antibody were prepared by incubating 2 mcl of 1.1 micromolar Streptavidin-ZAP (Biotin Z Internalization Kit) and 2 mcl of 1.1 micromolar biotinylated antibody for 30 min at room temperature.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Rosen S, Ham B, Drouin S, Boachie N, Chabot-Dore A, Austin J, Diatchenko L, Mogil J (2017) T-cell mediation of pregnancy analgesia affecting chronic pain in mice. J Neurosci 37:9819-9827.. doi: 10.1523/JNEUROSCI.2053-17.2017

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)

Schiroli G, Ferrari S, Conway A, Jacob A, Capo V, Albano L, Plati T, Castiello M, Sanvito F, Gennery A, Bovolenta C, Palchaudhuri R, Scadden D, Holmes M, Villa A, Sitia G, Lombardo A, Genovese P, Naldini L (2017) Preclinical modeling highlights the therapeutic potential of hematopoietic stem cell gene editing for correction of SCID-X1. Sci Transl Med 9(411):eaan0820. doi: 10.1126/scitranslmed.aan0820

Objective: To study potential approaches to gene therapy in mouse models of severe combined immunodeficiency.

Summary: The threshold of IL2RG gene editing can be reached for safe and efficient correction of SCID-X1 established in a preclinical model in human long-term repopulating HSPCs.

Usage: Biotinylated Anti-CD45 was mixed equimolar to Streptavidin-ZAP and administered as a single dose which caused substantial depletion (~70%) of the HSPC compartments and milder depletion of the more mature cell populations.

Related Products: Streptavidin-ZAP (Cat. #IT-27), Anti-CD45.2-SAP (Cat. #IT-91)

Therapeutic Potential of Hematopoietic Stem Cell Gene Editing

Cai S, Tsui YP, Tam KW, Shea GK, Chang RS, Ao Q, Shum DK, Chan YS (2017) Directed differentiation of human bone marrow stromal cells to fate-committed schwann cells. Stem Cell Reports 9(4):1097-1108. doi: 10.1016/j.stemcr.2017.08.004 PMID: 28890164

Objective: To create a protocol for in vitro derivation of fate-committed Schwann cells (SCs) from human bone marrow stromal cells (BMSCs).

Summary: This study demonstrates that the human bone marrow harbors neuro-ectodermal progenitors that can be enriched, expanded, and directed to differentiate into functionally mature, fate-committed SCs.

Usage: IF (1:500) and WB (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)