References

Chen PC, Hsieh MH, Kuo WS, Kao HF, Hsu CL, Wang JY (2017) Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis. J Allergy Clin Immunol 140(4):1146-1149.e8. doi: 10.1016/j.jaci.2017.03.022 PMID: 28412394

Objective: To evaluate the effect of extensive water-soluble chitosan (EWSC) in Allergic rhinitis (AR).

Summary: The results suggest that NGF- and TH2-related cytokines created a positive feedback loop amplifying allergic inflammation in the upper-airway epithelial cells, and EWSC attenuated allergic inflammation and epithelial cell damage, by inhibiting NGF biosynthesis during allergic TH2 immune responses.

Usage: IHC

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Cesarini V, Guida E, Todaro F, Di Agostino S, Tassinari V, Nicolis S, Favaro R, Caporali S, Lacal PM, Botti E, Costanzo A, Rossi P, Jannini EA, Dolci S (2017) Sox2 is not required for melanomagenesis, melanoma growth and melanoma metastasis in vivo. Oncogene 36(31):4508-4515. doi: 10.1038/onc.2017.53 PMID: 28368402

Objective: To investigate whether Sox2 is required during the process of melanomagenesis, melanoma growth and metastasis and in the acquisition of resistance to BRAF inhibitors (BRAFi) treatments.

Summary: The findings exclude an oncogenic function for Sox2 during melanoma development and do not support a role for this transcription factor in the acquisition of resistance to BRAFi treatments.

Usage: Immunofluorescence on melanoma cell lines

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Barry DM, Munanairi A, Chen Z-F (2018) Spinal mechanisms of itch transmission. Neurosci Bull 34:156-164. doi: 10.1007/s12264-017-0125-2

Related Products: Bombesin-SAP (Cat. #IT-40)

Huang L, Yuan T, Tan M, Xi Y, Hu Y, Tao Q, Zhao Z, Zheng J, Han Y, Xu F, Luo M, Sollars P, Pu M, Pickard G, So K, Ren C (2017) A retinoraphe projection regulates serotonergic activity and looming-evoked defensive behaviour. Nat Commun 8:14908. doi: 10.1038/ncomms14908 PMID: 28361990

Objective: To investigate how the dorsal raphe nucleus (DRN) and superior colliculus work in concert to extract and translate visual threats into defensive behavioural responses.

Summary: A dedicated population of RGCs signals rapidly approaching visual threats and their input to the DRN controls a serotonergic self-gating mechanism that regulates innate defensive responses.

Usage: Mice received bilateral intraocular injection (2 μg per eye) made between Streptavidin-Saporin and a biotinylated CTB antibody, or Anti-Melanopsin-SAP (2 μg per eye). For detection of melanopsin, retinas were incubated for 3 days at 4 °C with anti-melanopsin (1:600).

Related Products: Streptavidin-ZAP (Cat. #IT-27), Melanopsin-SAP (Cat. #IT-44), Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Malheiros-Lima M, Takakura A, Moreira T (2017) Depletion of rostral ventrolateral medullary catecholaminergic neurons impairs the hypoxic ventilatory response in conscious rats. Neuroscience 351:1-14.. doi: 10.1016/j.neuroscience.2017.03.031

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Kobayashi K, Maezawa T, Tanaka H, Onuki H, Horiguchi Y, Hirota H, Ishida T, Horiike K, Agata Y, Aoki M, Hoshi M, Matsumoto M (2017) The identification of ᴅ-tryptophan as a bioactive substance for postembryonic ovarian development in the planarian Dugesia ryukyuensis. Sci Rep 7:45175. doi: 10.1038/srep45175 PMID: 28338057

Usage: IHC 1:50

Ye J-H, Fu R, He W (2017) The rostromedial tegmental nucleus and alcohol addiction. Oncotarget 8:18624-18625.. doi: 10.18632/oncotarget.15822

Summary: The authors discuss their work with Dermorphin-SAP (Cat. #IT-12) and their demonstration that damage of RMTg MOR-expressing GABAergic neurons by Dermorphin-SAP increased the intake and preference for alcohol, boosted the expression and slowed down the extinction of alcohol conditioned place preference, and increased locomotion. Microinjection of DS into the RMTg substantially reduced the number of RMTg cells. Importantly, the rats that received DS injection elevated their alcohol intake and preference compared to those that received an injection of Blank-SAP (Cat. #IT-21), which did not cause neuronal damage.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Blank-SAP (Cat. #IT-21)

Ekman M, Zhu B, Swärd K, Uvelius B (2017) Neurite outgrowth in cultured mouse pelvic ganglia – Effects of neurotrophins and bladder tissue. Auton Neurosci 205:41-49. doi: 10.1016/j.autneu.2017.03.004 PMID: 28347639

Objective: To characterize the effects of the neurotrophins nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3) on the sprouting rate of sympathetic and parasympathetic neurites from the female mouse ganglion.

Summary: All three factors (BDNF, NT-3 and NGF) stimulated neurite outgrowth of both sympathetic and parasympathetic neurites although BDNF and NT-3 had a higher stimulatory effect on parasympathetic ganglion cells. Active forms of BDNF and NT-3 were detected in urinary bladder tissue, whereas tissue from the diaphragm expressed NGF.

Usage: in vitro culture (2 ul/ml)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Gupta A, Anjomani-Virmouni S, Koundouros N, Dimitriadi M, Choo-Wing R, Valle A, Zheng Y, Chiu YH, Agnihotri S, Zadeh G, Asara JM, Anastasiou D, Arends MJ, Cantley LC, Poulogiannis G (2017) PARK2 depletion connects energy and oxidative stress to PI3K/Akt activation via PTEN S-Nitrosylation. Mol Cell 65(6):999-101.e7. doi: 10.1016/j.molcel.2017.02.019 PMID: 28306514

Objective: To investigate the missing piece in the dynamic signaling and metabolic network governing PI3K/Akt activation.

Summary: PARK2 inactivation connects energy and oxidative stress to Akt activation via redox-mediated inactivation of PTEN by S-nitrosylation to support cell survival under conditions of energy deprivation.

Usage: Immunoblotting

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Gholamin S, Mitra SS, Feroze AH, Liu J, Kahn SA, Zhang M, Esparza R, Richard C, Ramaswamy V, Remke M, Volkmer AK, Willingham S, Ponnuswami A, McCarty A, Lovelace P, Storm TA, Schubert S, Hutter G, Narayanan C, Chu P, Raabe EH, Harsh G 4th, Taylor MD, Monje M, Cho YJ, Majeti R, Volkmer JP, Fisher PG, Grant G, Steinberg GK, Vogel H, Edwards M, Weissman IL, Cheshier SH (2017) Disrupting the CD47-SIRPα anti-phagocytic axis by a humanized anti-CD47 antibody is an efficacious treatment for malignant pediatric brain tumors. Sci Transl Med 9(381):eaaf2968. doi: 10.1126/scitranslmed.aaf2968 PMID: 28298418

Summary: A humanized anti-CD47 antibody has proven to be an effective treatment for malignant pediatric brain tumors by disrupting the CD47-SIRPα anti-phagocytic axis. This breakthrough therapy shows promise in improving outcomes for young patients with these challenging tumors.