References

Havelin J, Imbert I, Sukhtankar D, Remeniuk B, Pelletier I, Gentry J, Okun A, Tiutan T, Porreca F, King T (2017) Mediation of movement-induced breakthrough cancer pain by IB4-binding nociceptors in rats. J Neurosci 37:5111-5122.. doi: 10.1523/JNEUROSCI.1212-16.2017

Objective: To define a novel preclinical measure of movement-induced breakthrough pain (BTP) that is observed in the presence of morphine controlling ongoing pain.

Summary: Novel compounds targeting IB4-binding nociceptors may improve pain management for cancer pain patients and other patient populations suffering from BTP that is inadequately treated by currently available medications.

Usage: To determine the effect of eliminating input from IB4-binding fibers, separate groups of rats received spinal administration of IB4-SAP or the control, Blank-SAP (3.2 mcg/20 mcl saline) followed by a 10 mcl flush of saline. Movement of an air bubble placed between drug solution and saline was used to monitor progress of the injection.

Related Products: IB4-SAP (Cat. #IT-10), Blank-SAP (Cat. #IT-21)

Wu W, Wu XL, Ji YQ, Gao Z (2017) Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro. Mol Med Rep 16(1):95-100. doi: 10.3892/mmr.2017.6585 PMID: 28534946

Objective: To isolate nestin-negative outer root sheath cells (ORSCs) from adult healthy volunteers and investigate the neuronal differentiation of these cells.

Summary: The present study did not verify the value of neural differentiation into sphere-forming cells for therapeutic application, however, the differentiation of nestin-negative human hair follicle ORSCs into neurons in vitro may serve as a theoretical underpinning for neurodegenerative diseases and regenerative medicine.

Usage: IF (1:250)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Dorard C, Estrada C, Barbotin C, Larcher M, Garancher A, Leloup J, Beermann F, Baccarini M, Pouponnot C, Larue L, Eychène A, Druillennec S (2017) RAF proteins exert both specific and compensatory functions during tumour progression of NRAS-driven melanoma. Nat Commun 8:15262. doi: 10.1038/ncomms15262 PMID: 28497782

Objective: To investigate the contribution of Raf genes in tumor progression, from the onset of benign tumors to malignant tumor maintenance, in NRAS-induced mouse melanoma models

Summary: BRAF expression is required for ERK activation and nevi development, demonstrating a critical role in the early stages of NRAS-driven melanoma. After melanoma formation, single BRAF or CRAF ablation is not sufficient to block tumor growth, showing redundant functions for RAF kinases. Proliferation of resistant cells emerging in the absence of BRAF and CRAF remains dependent on ARAF-mediated ERK activation.

Usage: Cell sorting by Flow Cytometry (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Guo JR, Wang H, Jin XJ, Jia DL, Zhou X, Tao Q (2017) Effect and mechanism of inhibition of PI3K/Akt/mTOR signal pathway on chronic neuropathic pain and spinal microglia in a rat model of chronic constriction injury. Oncotarget 8(32):52923-52934. doi: 10.18632/oncotarget.17629 PMID: 28881783

Objective: To explore the effects of inhibition of PI3K/Akt/mTOR signal pathway on chronic neuropathic pain (CNP) and spinal microglia in a rat model of chronic constriction injury (CCI).

Summary: Inhibition of PI3K/Akt/mTOR signal pathway may alleviate CNP and reduce microglia and GFAP and NGF expressions in marrow in a rat model of CCI.

Usage: immunofluorescence assay (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kulkarni S, Micci MA, Leser J, Shin C, Tang SC, Fu YY, Liu L, Li Q, Saha M, Li C, Enikolopov G, Becker L, Rakhilin N, Anderson M, Shen X, Dong X, Butte MJ, Song H, Southard-Smith EM, Kapur RP, Bogunovic M, Pasricha PJ (2017) Adult enteric nervous system in health is maintained by a dynamic balance between neuronal apoptosis and neurogenesis. Proc Natl Acad Sci U S A 114(18):E3709-E3718. doi: 10.1073/pnas.1619406114 PMID: 28420791

Objective: To identify adult enteric neural precursor cell (ENPC) that would enable a new understanding of the pathogenesis of enteric neuromuscular diseases as well as the development of novel regenerative therapies.

Summary: The results show significant turnover and neurogenesis of adult enteric neurons and provide a paradigm for understanding the enteric nervous system in health and disease.

Usage: IHC (1:400)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Jenrette T, Logue J, Horner KA (2017) Striatal patch compartment lesions reduce habitual behaviors. FASEB J 31(S1):1059.6. Experimental Biology 2017 Meeting Abstracts. doi: 10.1096/fasebj.31.1_supplement.1059.6

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)

Erdos B, Cruickshank NC, Einwag ZD, Schaich CL, Wellman TL (2017) Increased BDNF expression in the paraventricular nucleus of the hypothalamus (PVN) abolishes hypotensive actions of nucleus of the solitary tract (NTS) catecholaminergic neurons. FASEB J 31(S1):866.7. Experimental Biology 2017 Meeting Abstracts. doi: fasebj.31.1_supplement.866.7

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Craig TA, Brown SM, Nichols NL (2017) Adenosine 2A (A2A) receptor expression in rats with motor neuron death from intrapleural CTB-saporin injections. FASEB J 31(S1):873.14. Experimental Biology 2017 Meeting Abstracts. doi: 10.1096/fasebj.31.1_supplement.873.14

Related Products: CTB-SAP (Cat. #IT-14)

Nichols NL, Tanner M (2017) Phrenic long-term facilitation (pLTF) following acute intermittent hypoxia (AIH) is TrkB and PI3K/Akt dependent in rats with motor neuron death from intrapleural CTB-saporin injections. FASEB J 31(S1):1053.13. Experimental Biology 2017 Meeting Abstracts. doi: 10.1096/fasebj.31.1_supplement.1053.13

Related Products: CTB-SAP (Cat. #IT-14)

Gulino R, Parenti R, Gulisano M (2017) Sonic hedgehog and TDP-43 participate in the spontaneous locomotor recovery in a mouse model of spinal motoneuron disease. J Funct Morphol Kinesiol 2:11.. doi: 10.3390/jfmk2020011

Summary: The authors used a mouse model of neurotoxic motoneuron depletion to study the role of Sonic hedgehog and TDP-43 in the compensatory changes occurring after the lesion. The injection of cholera toxin-B saporin (CTB-SAP; Cat. #IT-14) into the gastrocnemius muscle caused a partial motoneuron death accompanied by an impairment of locomotion.  Motor activity was significantly restored as soon as one month later.

Related Products: CTB-SAP (Cat. #IT-14)