References

Guo Y, Hu J, Wang Y, Peng X, Min J, Wang J, Matthaiou E, Cheng Y, Sun K, Tong X, Fan Y, Zhang PJ, Kandalaft LE, Irving M, Coukos G, Li C (2018) Tumour endothelial marker 1/endosialin-mediated targeting of human sarcoma. Eur J Cancer 90:111-121. doi: 10.1016/j.ejca.2017.10.035. PMID: 29304474

Objective: Using Saporin conjugated to 78Fc, an antibody fragment directed towards endosialin a.k.a. CD248, in the treatment of human sarcomas.

Summary: Endosialin/CD248 is over-expressed in human sarcomas, and a human single-chain variable fragment -Fc fusion protein was created targeting CD248. 78Fc-SAP eliminated CD248+ cells without off-target toxicity in vivo in mice and in vitro in human sarcoma cells.

Usage: In vivo: Mice were inoculated with osteosarcoma cells. These mice received, in PBS, either 4 ug of 78Fc-SAP, 1.4 ug of free saporin [PR-01], or no drug. In vitro: At 2.2 nM nearly 100% of CD248+ human sarcoma cell lines ( SJSA-1, A673, MES-SA and HOS) were eliminated. At the same concentration with free saporin ~20% of cells were eliminated.

Related Products: Saporin (Cat. #PR-01)

Stabio ME, Sabbah S, Quattrochi LE, Ilardi MC, Fogerson PM, Leyrer ML, Kim MT, Kim I, Schiel M, Renna JM, Briggman KL, Berson DM (2018) The M5 cell: A color-opponent intrinsically photosensitive retinal ganglion cell. Neuron 97:150-163. doi: 10.1016/j.neuron.2017.11.030 PMID: 29249284

Objective: To provide a fuller description of the least understood ipRGC type, the M5 cell, and discovered a distinctive functional characteristic— chromatic opponency (ultraviolet excitatory, green inhibitory).

Summary: M5 cells send axons to the dLGN and are thus positioned to provide chromatic signals to visual cortex. These findings under- score that melanopsin’s influence extends beyond unconscious reflex functions to encompass cortical vision, perhaps including the perception of color.

Usage: M5 cells have the weakest melanopsin responses of all known ipRGC types. The intrinsic melanopsin response (~10 pA) in M5 cells is at least an order of magnitude smaller than the extrinsic, synaptically mediated response.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Fujita M, Fukuda T, Sato Y, Takasusuki T, Tanaka M (2018) Allopregnanolone suppresses mechanical allodynia and internalization of neurokinin-1 receptors at the spinal dorsal horn in a rat postoperative pain model. Korean J Pain 31:10-15. doi: 10.3344/kjp.2018.31.1.10. PMID: 29372021

Objective: To identify a new strategy for postoperative pain management, this study investigated the analgesic effects of allopregnanolone (Allo) in an incisional pain model, and its effects on the activities of the primary afferent fibers at the dorsal horn.

Summary: Systemic administration of Allopregnanolone inhibited mechanical allodynia and activities of the primary afferent fibers at the dorsal horn in a rat postoperative pain model. Allopregnanolone was proposed as a candidate for postoperative pain management.

Usage: Fluorescence immunohistochemistry: Six sections per animal were labeled with primary antiserum, rabbit anti-NK-1R (1:3000).

Related Products: NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)

Review

Lund K, Olsen CE, Wong JJW, Olsen PA, Solberg NT, Høgset A, Krauss S, Selbo PK (2017) 5-FU resistant EMT-like pancreatic cancer cells are hypersensitive to photochemical internalization of the novel endoglin-targeting immunotoxin CD105-saporin. J Exp Clin Cancer Res 36(1):185.. doi: 10.1186/s13046-017-0662-6

Objective: Investigate resistance mechanisms and photochemical strategies to overcome 5-FU resistance in pancreatic adenocarcinoma.

Summary: Expression of CD105 was investigated using RT-qPCR, western blotting, flow cytometry, and fluorescence microscopy, and co-localization of TPCS2a and Anti-CD105-SAP was assessed using microscopy. MTS assay was used to investigate cytotoxic effects of photochemical internalization of Anti-CD105-SAP. For the first time, we demonstrate the promise of PCI-based targeting of CD105 in site-specific elimination of 5-FU resistant pancreatic cancer cells using Anti-CD105-SAP in vitro. PCI-based targeting of CD105 may represent a potent anti-cancer strategy and should be further evaluated in preclinical models.

Usage: Cells were seeded (3000/well) in 96-well plates and allowed to attach overnight. The cells were incubated with the Anti-CD105-saporin (2.4 nM) or Saporin as a control (6.48 nM; Saporin was added in a molecular ratio of 2.7:1 to the immunotoxin) giving an equal ratio of Saporin to immunotoxin), with or without the photosensitizer TPCS2a (0.35 μg/ml) for 18 h.

Related Products: Anti-CD105-SAP (Cat. #IT-80)

Thom G, Hatcher J, Hearn A, Paterson J, Rodrigo N, Beljean A, Gurrell I, Webster C (2018) Isolation of blood-brain barrier-crossing antibodies from a phage display library by competitive elution and their ability to penetrate the central nervous system. mAbs 10:304-314. doi: 10.1080/19420862.2017.1409320 PMID: 29182455

Summary: Fluorescence microvolume assay technology (FMAT) periprep extract or Nickel-purified scFvs from E. coli were transferred into assay plates containing 10 ml (1:1000 dilution of a 1 mg/ml stock solution) mouse anti-HIS MAb.)

Related Products: 6His Mouse Monoclonal (Cat. #AB-213)

Ullah K, Rosendahl AH, Izzi V, Bergmann U, Pihlajaniemi T, Maki JM, Myllyharju JA-Ohoo (2017) Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways. Sci Rep 7:172200. doi: 10.1038/s41598-017-17376-0 PMID: 29222481

Usage: Western blot

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Brick RM, Sun AX, Tuan RSA-Ohoo (2018) Neurotrophically induced mesenchymal progenitor cells derived from induced pluripotent stem cells enhance neuritogenesis via neurotrophin and cytokine production. Stem Cells Transl Med 7:45-58. doi: 10.1002/sctm.17-0108 PMID: 29215199

Objective: To examine the potential utility of an alternative, mesenchymal-like cell (MSCs) source, derived from induced pluripotent stem cells, termed induced mesenchymal progenitor cells (MiMPCs) to produce bioactive factors and support nerve regeneration.

Summary: The findings suggest MiMPCs as a renewable, candidate source of therapeutic cells and a potential alternative to MSCs for peripheral nerve repair, in view of their ability to promote nerve growth by producing many of the same growth factors and cytokines as Schwann cells and signaling through critical neurotrophic pathways.

Usage: Immunofluorescence

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Zhang J, Wang H, Wu S, Liu Q, Wang N (2017) Regulation of reentrainment function is dependent on a certain minimal number of intact functional iprgcs in rd mice. J Ophthalmol 2017:6804853.. doi: 10.1155/2017/6804853

Related Products: Melanopsin-SAP (Cat. #IT-44)

Khan N, Muralidharan A, Smith MT (2017) Attenuation of the infiltration of angiotensin ii expressing CD3+ T-cells and the modulation of nerve growth factor in lumbar dorsal root ganglia – a possible mechanism underpinning analgesia produced by EMA300, an Angiotensin II type 2 (AT2) receptor antagonist. Front Mol Neurosci 10:389. doi: 10.3389/fnmol.2017.00389 PMID: 29200998

Objective: To investigate the cellular and molecular mechanism of action of selective small molecule angiotensin II type 2 (AT2) receptor antagonists in the alleviation of peripheral neuropathic pain.

Summary: The analgesic effect of EMA300 in CCI-rats involves multimodal actions that appear to be mediated at least in part by a significant reduction in the otherwise increased expression levels of Ang II as well as the number of Ang II-expressing CD3+ T-cells in the ipsilateral lumbar DRGs of CCI-rats.

Usage: Immunocytochemistry; specifically labeled HEK cells expressing the AT2 but not the AT1 receptor or the non-transfected HEK-cells.

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Liu A, Aoki S, Wickens J (2017) A streamlined method for the preparation of gelatin embedded brains and simplified organization of sections for serial reconstructions. Bio-protocol 7(22):e2610.. doi: 10.21769/BioProtoc.2610

Related Products: Anti-ChAT-SAP (Cat. #IT-42)