References

Winston J (2018) Mo1546 – Neonatal colon inflammation-induced increases in pituitary adenylate cyclase activating peptide expression in the parabrachial nucleus contributes to reduced meal consumption, to increased meal-induced aversive and anxiety-like behaviors in adult rats. Gastroenterology 154:S-748. doi: 10.1016/S0016-5085(18)32597-6

Summary: Infusion of Anti-DBH-SAP into the dorsal vagal complex significantly increased meal volumes and increased open field activity compared to IgG-saporin treatment.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Meng J, Ni J, Wu Z, Jiang M, Zhu A, Qing H, Nakanishi H (2018) The critical role of IL-10 in the antineuroinflammatory and antioxidative effects of Rheum tanguticum on activated microglia. Oxid Med Cell Longev 2018:12. doi: 10.1155/2018/1083596

Objective: To investigate anti-inflammatory and antioxidative effects of a traditional Tibetan medicine (Rheum tanguticum; RT) on activated microglia.

Summary: RT may be useful for the pharmacological intervention against excessive inflammatory and oxidative responses associated with AD by inducing the production of IL-10 by microglia.

Usage: Mac-1-SAP (Cat. #IT-06, 1.3nM) was applied to hippocampal slice cultures 24 h prior to stimulation with chromogranin A or pancreastatin.

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)

Ogawa T, Li Y, Lua I, Hartner A, Asahina K (2018) Isolation of a unique hepatic stellate cell population expressing integrin α8 from embryonic mouse livers. 247(6):867-881. doi: 10.1002/dvdy.24634 PMID: 29665133

Objective: The aim of this study is to identify specific markers for embryonic Hepatic stellate cells (HSCs).

Summary: ITGA8 is a specific cell surface marker of MC-derived HSCs and perivascular mesenchymal cells in the developing liver. Our data suggest that ITGA8+ mesenchymal cells maintain the phenotype of hepatoblast in liver development.

Usage: Immunofluorescence (1:1000)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Ljubojevic V, Luu P, Gill PR, Beckett L-A, Takehara-Nishiuchi K, De Rosa E (2018) Cholinergic modulation of frontoparietal cortical network dynamics supporting supramodal attention. J Neurosci 38:3988-4005. doi: 10.1523/JNEUROSCI.2350-17.2018

Objective: To examine whether neurochemical acetylcholine (ACh) contributes to a state of readiness for target detection, by engaging frontoparietal cortical attentional networks independent of modality.

Summary: ACh supported alerting attention to an impending presentation of either visual or olfactory targets. Enhanced stimulus detection was associated with an anticipatory increase in power in the beta oscillation range prior to the target’s appearance within the posterior parietal cortex (PPC) as well as increased synchrony. In beta, this was also detected between the prefrontal cortex and PPC. ACh may act, in a supramodal manner, to prepare frontoparietal cortical attentional networks for target detection.

Usage: The ACh-NBM-lesioned rats received an injection of 0.2 μl of 0.3 μg/μl 192 IgG-SAP

Related Products: 192-IgG-SAP (Cat. #IT-01)

Zhang Q, De Corte B, Jung D, Kim Y, Geerling J, Narayanan N (2018) Cholinergic modulation targeting medial prefrontal cortex leads to behavior deficit in interval timing task. Neurology 90 (15 Supplement):P5.195.

Objective: To determine the effect of cholinergic lesion targeting medial prefrontal cortex on interval timing behavior.

Summary: Mice receiving medial prefrontal mu-p75-saporin injection performed poorly compared to control mice in interval timing task. Cholinergic lesion targeting medial prefrontal cortex caused interval timing behavior deficit in wild type mice.

Usage: mu-p75-SAP, a toxin targeting cholinergic neurons, into the bilateral medial prefrontal cortical regions of wild type mice pre-trained in interval timing task. Control mice (also pre-trained in interval timing task, n=8) received stereotactic injection of Rabbit IgG-SAP.

Related Products: mu p75-SAP (Cat. #IT-16), Rabbit IgG-SAP (Cat. #IT-35)

Chen SX, Wang SK, Yao PW, Liao GJ, Na XD, Li YY, Zeng WA, Liu XG, Zang Y (2018) Early CALP2 expression and microglial activation are potential inducers of spinal IL-6 upregulation and bilateral pain following motor nerve injury. J Neurochem 145:154-169. doi: 10.1111/jnc.14317

Related Products: Mac-1-SAP rat (Cat. #IT-33)

Watanabe S, Sakurai T, Nakamura S, Miyoshi K, Sato M (2018) The combinational use of CRISPR/Cas9 and targeted toxin technology enables efficient isolation of bi-allelic knockout non-human mammalian clones. Int J Mol Sci 19:E1075. doi: 10.3390/ijms19041075

Objective: Most genome editing systems employ transient treatment with selective drugs such as puromycin to obtain the desired genome-edited cells, which often allows some untransfected cells to survive and decreases the efficiency of generating genome-edited cells. The authors developed a novel targeted toxin-based drug-free selection system for the enrichment of genome-edited cells.

Summary: Results indicate that a combination of the CRISPR/Cas9 system and targeted toxin technology using IB4-SAP allows efficient enrichment of genome-edited clones, particularly bi-allelic KO clones.

Usage: Cells were trypsinized 3 days after transfection and approximately 80% were incubated for 30 min at 37°C in a solution (25 mcL) containing 0.5–1.0 mcg IB4-SAP (Cat. #IT-10).

Related Products: IB4-SAP (Cat. #IT-10)

Bassil M, Atat AA, Ibragim M (2018) Detection of oxidative stress in buccal cells using iSWAB tubes. J Mol Biomark Diagn 9(2):1-6. doi: 10.4172/2155-9929.1000387 PMID: 909090

Objective: To investigate the oxidative profile of iSWAB-Mawi protein tubes by examining their ability to detect the protein oxidation biomarkers: nitrotyrosine and S-nitrosocysteine in buccal cells.

Summary: Results showed the presence of S-nitrosocysteine and nitrotyrosine in buccal cells reflecting the ability of iSWAB-Mawi protein tubes to detect those protein oxidation biomarkers.

Usage: Western Blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Song SY, Zhang LC (2018) The establishment of a CSF-contacting nucleus “knockout” model animal. Front Neuroanat 12:22. doi: 10.3389/fnana.2018.00022

Objective: To establish a cerebrospinal fluid (CSF)-contacting nucleus-deficient model animal using cholera toxin B subunit-saporin (CTB-SAP).

Summary: The complete ablation occurred by Day 7 after CTB-SAP microinjection. A model animal that had no CSF-contacting nucleus was established after survival beyond that time point. No obvious effects were observed in the vital status of the model animals, and their survival was ensured. The common physiological parameters of model animals were stable. The present study provides a method to establish a CSF-contacting nucleus “knockout” model animal, which is similar to a gene knockout model animal for studying this particular nucleus in vivo.

Usage: 3 μl (500 ng) CTB-SAP was microinjected into the lateral ventricle.

Related Products: CTB-SAP (Cat. #IT-14)

Patrone LGA, Biancardi V, Marques DA, Bícego KC, Gargaglioni LH (2018) Brainstem catecholaminergic neurones and breathing control during postnatal development in male and female rats. J Physiol 596(15):3299-3325. doi: 10.1113/JP275731

Objective: To determine the role of the brainstem CA system in ventilatory control under normocapnic and hypercapnic conditions during different phases of development (P7-8, P14-15 and P20-21) in male and female Wistar rats.

Summary: Brainstem CA neurones produce a tonic inhibitory drive that affects breathing frequency in P7-8 rats and provide an inhibitory drive during hypercapnic conditions in both males and females at P7-8 and P14-15.

Usage: Anti-DBH-SAP (420 ng/μL – 1 μL for P0-1; 1.5 μL for P7-8; 2.0 μL for P13-14) was injected into the 4th ventricle of neonatal Wistar rats of both sexes. Control rats were injected with vehicle (PBS, 0.01 M, pH 7.4) or unconjugated saporin (Cat. #PR-01), with respective volumes for each age, as described for the Anti-DBH-SAP group. All injections were performed using a microinjector pump over a period of 5 min to allow drug diffusion.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Saporin (Cat. #PR-01)