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The establishment of a CSF-contacting nucleus “knockout” model animal.

Song SY, Zhang LC (2018) The establishment of a CSF-contacting nucleus “knockout” model animal. Front Neuroanat 12:22. doi: 10.3389/fnana.2018.00022

Objective: To establish a cerebrospinal fluid (CSF)-contacting nucleus-deficient model animal using cholera toxin B subunit-saporin (CTB-SAP).

Summary: The complete ablation occurred by Day 7 after CTB-SAP microinjection. A model animal that had no CSF-contacting nucleus was established after survival beyond that time point. No obvious effects were observed in the vital status of the model animals, and their survival was ensured. The common physiological parameters of model animals were stable. The present study provides a method to establish a CSF-contacting nucleus “knockout” model animal, which is similar to a gene knockout model animal for studying this particular nucleus in vivo.

Usage: 3 μl (500 ng) CTB-SAP was microinjected into the lateral ventricle.

Related Products: CTB-SAP (Cat. #IT-14)