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Brainstem catecholaminergic neurones and breathing control during postnatal development in male and female rats.

Patrone LGA, Biancardi V, Marques DA, Bícego KC, Gargaglioni LH (2018) Brainstem catecholaminergic neurones and breathing control during postnatal development in male and female rats. J Physiol 596(15):3299-3325. doi: 10.1113/JP275731

Objective: To determine the role of the brainstem CA system in ventilatory control under normocapnic and hypercapnic conditions during different phases of development (P7-8, P14-15 and P20-21) in male and female Wistar rats.

Summary: Brainstem CA neurones produce a tonic inhibitory drive that affects breathing frequency in P7-8 rats and provide an inhibitory drive during hypercapnic conditions in both males and females at P7-8 and P14-15.

Usage: Anti-DBH-SAP (420 ng/μL – 1 μL for P0-1; 1.5 μL for P7-8; 2.0 μL for P13-14) was injected into the 4th ventricle of neonatal Wistar rats of both sexes. Control rats were injected with vehicle (PBS, 0.01 M, pH 7.4) or unconjugated saporin (Cat. #PR-01), with respective volumes for each age, as described for the Anti-DBH-SAP group. All injections were performed using a microinjector pump over a period of 5 min to allow drug diffusion.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Saporin (Cat. #PR-01)