References

Suarez AN, Hsu TM, Liu CM, Noble EE, Cortella AM, Nakamoto EM, Hahn JD, de Lartigue G, Kanoski SE (2018) Gut vagal sensory signaling regulates hippocampus function through multi-order pathways. Nat Commun 9(1):2181. doi: 10.1038/s41467-018-04639-1

Objective: To determine the  endogenous relevance of GIderived vagal HPC communication.

Summary: Endogenous derived vagal sensory signaling promotes HPC-dependent memory function via a multi-order brainstem–septal pathway, thereby identifying a previously unknown role for the gut–brain axis in memory control.

Usage: A 1-µl volume of CCK-SAP (250 ng/µl) or control Saporin (250 ng/µl) was injected at two sites: 0.5 µl rostral and 0.5 µl caudal to the laryngeal nerve branch.

Related Products: CCK-SAP (Cat. #IT-31), Saporin (Cat. #PR-01)

Sadhukhan R, Brown N, Ouellette D, Banach D, Filoti DI, Winarta D, Raghavendra R, Sousa S, Darcy A, Alessandri L, Ivanov A, Bose S, Eaton L, Preston G, Freeman J, Correia I (2018) Engineering elastic properties into an anti-TNFα monoclonal antibody. Cogent Biol 4(1):1469387. doi: 10.1080/23312025.2018.1469387

Objective: To engineer elastic properties into a TNFalpha antibody.

Summary: The results presented in this report with an anti-TNFα ELP mAb are a foundation for building on a new generation of fusion ELP mAbs, or other formats, that are stable, active, responsive to cues in local environment, and, with the FcRn mutation, cleared rapidly from circulation. More detailed studies are warranted to identify the appropriate ELP sequences for IA delivery, calculate residence time in the IA space, and demonstrate pharmacodynamics effect of the ELP-fusion protein.

Usage: Fab-ZAP human was mixed with anti-TNFα-ELP fusion monoclonal.

Related Products: Fab-ZAP human (Cat. #IT-51)

Sánchez-Migallón MC, Valiente-Soriano FJ, Nadal-Nicolás FM, Di Pierdomenico J, Vidal-Sanz M, Agudo-Barriuso M (2018) Survival of melanopsin expressing retinal ganglion cells long term after optic nerve trauma in mice. Exp Eye Res 174:93-97. doi: 10.1016/j.exer.2018.05.029 PMID: 29856984

Summary: Melanopsin-positive retinal ganglion cells (RGCs) do not respond to axotomy in the same way than the rest of RGCs, and so while image-forming RGCs die in two exponential phases, non-image forming RGCs die only during the first quick phase.

Usage: Retinas were dissected as flat mounts and double-immunostained against melanopsin (1:5000).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Motohashi T, Kunisada T (2019) Direct conversion of mouse embryonic fibroblasts into neural crest cells. (eds. Turksen K). In: Skin Stem Cells. Methods in Molecular Biology. 1879:307-321. Humana Press, New York, NY. doi: 10.1007/7651_2018_145 PMID: 29797008

Objective: To describe methods for the direct conversion of mouse embryonic fibroblasts into neural crest cells (NCCs).

Summary: Sox10-IRES-Venus mouse fibroblasts were used for the conversion and isolation of converted NCCs as SOX10-positive cells.

Usage: Immunostaining for Flow Cytometry

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Staib JM, Della Valle R, Knox DK (2018) Disruption of medial septum and diagonal bands of Broca cholinergic projections to the ventral hippocampus disrupt auditory fear memory. Learn Mem 152:71-79. doi: 10.1016/j.nlm.2018.05.009

Objective: To determine which efferent projections are critical for contextual fear memory discrimination and extinction memory.

Summary: The results of this study suggest that MS/vDBB cholinergic neurons are critical for fear and extinction memory.

Usage: 192-IgG saporin was infused into all brain regions at a concentration of 0.2 μg/μL dissolved in 0.2 M PBS. The total volume of each injection was 0.5 μL. Sham surgeries were accomplished using the same volume (0.5 μL) of PBS.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Dhez AC, Benedetti E, Antonosante A, Panella G, Ranieri B, Florio TM, Cristiano L, Angelucci F, Giansanti F, Di Leandro L, d’Angelo M, Melone M, De Cola A, Federici L, Galzio R, Cascone I, Raineri F, Cimini A, Courty J, Giordano A, Ippoliti R (2018) Targeted therapy of human glioblastoma via delivery of a toxin through a peptide directed to cell surface nucleolin. J Cell Physiol 233(5):4091-4105. doi: 10.1002/jcp.26205 PMID: 28941284

Wüstemann T, Haberkorn U, Babich J, Mier W (2019) Targeting prostate cancer: Prostate-specific membrane antigen based diagnosis and therapy. Med Res Rev 39(1):40-69. doi: 10.1002/med.21508 PMID: 29771460

Summary: Conjugation to the antibody was achieved by reacting the biotinylated humanized antibody to prostate-specific membrane antigen (PMSA) with Streptavidin-ZAP. Binding potency of the conjugate was comparable to that of the naked antibody and in vivo experiments proved potent for selective tumor growth inhibition in mice bearing LNCaP tumors.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

See Also:

• Kuroda K et al. Saporin toxin-conjugated monoclonal antibody targeting prostate-specific membrane antigen has potent anticancer activity. Prostate 70(12):1286-1294, 2010.

Eng MS, Kaur J, Prasmickaite L, Engesaeter BO, Weyergang A, Skarpen E, Berg K, Rosenblum MG, Maelandsmo GM, Hogset A, Ferrone S, Selbo PK (2018) Enhanced targeting of triple-negative breast carcinoma and malignant melanoma by photochemical internalization of CSPG4-targeting immunotoxins. Photochem Photobiol Sci 17:539-551. doi: 10.1039/C7PP00358G

Summary: The combination of the drug delivery technology PCI and CSPG4-targeting immunotoxins is an efficient, specific and light-controlled strategy for the elimination of aggressive cells of TNBC and malignant melanoma origin. This study lays the foundation for further preclinical evaluation of PCI in combination with CSPG4-targeting.

Usage: To obtain the immunotoxin 225.28-saporin, Streptavidin-Saporin (Cat. #IT-27; Streptavidin-ZAP), with an average of 2.5 molecules of saporin per molecule of streptavidin, was combined with biotinylated 225.28, a CSPG4-specific mouse mAb, IgG2a.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Sun X, Zhu Y, Yin HY, Guo ZY, Xu F, Xiao B, Jiang WL, Guo WM, Meng HY, Lu SB, Wang Y, Peng J (2018) Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function. Stem Cell Res Ther 9:133. doi: 10.1186/s13287-018-0884-3 PMID: 29751848

Objective: To improve the traditional methods for inducing the differentiation of mesenchymal stromal cells (MSCs) into Schwann cell-like cells (SCLCs).

Summary: Results indicated that the intermittent induction method is more efficient than traditional methods for inducing adipose-derived stem cells to differentiate into SCLCs.

Usage: immunofluorescence (1:500)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Vetter I (2018) Toxins in Neurobiology: New tools from old molecules. Neurosci Lett 679:1-3. doi: 10.1016/j.neulet.2018.05.008

Summary: The selective ablation of neurokinin-1 receptor-expressing neurons by SP-SAP revealed a key role for the preBötzinger complex in the generation of respiratory rhythm. Toxin-mediated neuronal ablation may also find therapeutic applications, such as the treatment of severe refractory pain in terminally ill patients by intrathecal SP-SAP which causes selective loss of neurokinin-1 receptor-expressing neurons in the spinal cord dorsal horn.

Related Products: SSP-SAP (Cat. #IT-11)