References

Souza GMPR, Kanbar R, Stornetta DS, Abbott SBG, Stornetta RL, Guyenet PG (2018) Breathing regulation and blood gas homeostasis after near complete lesions of the retrotrapezoid nucleus in adult rats. J Physiol 596(13):2521-2545. doi: 10.1113/JP275866

Objective: To test how important the retrotrapezoid nucleus (RTN) is to PCO2 homeostasis and breathing during sleep or wake.

Summary: Near complete RTN destruction in rats virtually eliminates the CRC but HVR persists and sighing and the state-dependence of breathing are unchanged. Under normoxia, RTN lesions cause no change in VE but alveolar ventilation is reduced by at least 21%, probably because of increased physiological dead volume. RTN lesions do not cause sleep apnea during SWS, even under hyperoxia.

Usage: A total of 6 microinjections (120 nl/injection; 3 rostrocaudally aligned injections per side) were made 100-200 μm below the lower edge of the facial motor nucleus 2 mm lateral to the midline. Experimental rats received either 0.6 ng, 1.2 ng, or 2.4 ng of SSP-SAP per injection.

Related Products: SSP-SAP (Cat. #IT-11)

Chiang JT, Badrealam KF, Shibu MA, Kuo CH, Huang CYA-Ohoo, Chen BC, Lin YM, Viswanadha VP, Kuo WW, Huang CY (2018) Eriobotrya japonica ameliorates cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats. Environ Toxicol 33:1113-1122. doi: 10.1002/tox.22589 PMID: 29974613

Objective: To evaluate the prospective efficacy of E. japonica leave extract (EJLE) against Angiotensin-II induced cardiac hypertrophy.

Summary: Supplementation of EJLE could rescue Ang-II induced cardiac hypertrophy.

Usage: Western blot, immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Hoffmann RM, Crescioli S, Thurston DE, Karagiannis SN (2018) Development and evaluation of T-Zap: a novel antibody-drug conjugate for the treatment of Her2 positive breast cancer. Cancer Res 78:LB-001. doi: 10.1158/1538-7445.AM2018-LB-001 PMID: 909090

Objective: Develop and Evaluate a novel ADC (T-Zap) for breast cancer.

Summary: Binding to target cells of T-Zap was confirmed. Comparison of T-Zap efficacy in breast cancer cell lines with and without resistance against trastuzumab showed a trend for higher efficacy of cell killing by T-Zap in trastuzumab resistant cells compared to T-DM1. Toxicity assays revealed no impact of T-Zap on cell viability in immune cells.

Usage: T-ZAP was made using Biotinylated monoclonal antibody trastuzumab mixed with Streptavidin-ZAP.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Ritter S (2018) Why I can’t say “no” to hindbrain catecholamine neurons. Appetite 126:210. doi: 10.1016/j.appet.2018.02.035

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Zhu Y, Zhao J, Han Q, Wang Z, Wang Z, Dong X, Li J, Liu L, Shen X (2018) The effect and mechanism of chinese herbal formula Sini Tang in heart failure after myocardial infarction in rats. Evid Based Complement Alternat Med 2018:5629342. doi: 10.1155/2018/5629342 PMID: 30050591

Objective: To investigate the effectiveness and mechanism of theChinese herbal formula Sini Tang (SNT) in heart failure after myocardial infarction in rats.

Summary: The Chinese herbal formula SNT could improve left ventricular systolic function in heart failure after myocardial infarction.

Usage: Western blot.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Kim M, Vargas M, Harlan B, Killoy K, Ball L, Comte-Walters S, Gooz M, Yamamoto Y, Beckman J, Barbeito L, Pehar M (2018) Nitration and glycation turn mature NGF into a toxic factor for motor neurons: A role for p75. Antioxid Redox Signal 28:1587-1602. doi: 10.1089/ars.2016.6966 PMID: 28537420

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Harvey BP, Cohen-Solal J, Kaymakcalan Z (2018) SAT0058 Adalimumab: TNF complexes are cleared more efficiently by human osteoclasts than those with etanercept through FCG-receptor binding and internalisation. Ann Rheum Dis 77:893. EULAR 2018, Amsterdam, The Netherlands doi: 10.1136/annrheumdis-2018-eular.3804

Objective: To determine whether Fc-gamma receptor (FcgR)-mediated internalization of the biologic:TNF complexes is a contributing mechanism responsible for the difference in effectiveness between ADA and ETN in preventing TNF- enhanced OCgenesis.

Summary: Human osteoclast  (OC) precursors can bind and internalise ADA:TNF complexes more efficiently than ETN:TNF complexes. In addition, this process is partially mediated through FcgRII.

Usage: FcgR-mediated nternalization was assessed by monitoring a reduction in OC survival in response to preformed bio- logic: TNF complexes (25:1 ratio) bound with FabFC-ZAP human ± FcgR blocking antibodies.

Related Products: FabFc-ZAP human (Cat. #IT-65)

Sparks NR (2018) The embryotoxic effects of harm reduction tobacco products on osteoblasts developing from human embryonic stem cells. University of California Riverside Thesis.

Objective: To investigate the effect of maternal smoking in the development of embryonic skeleton and the molecular mechanisms involved in skeletal embryotoxicity induced by conventional and harm-reduction tobacco.

Summary: An in vitro model based on human embryonic stem cells was developed, that mimics the development of the human embryo; their differentiation into osteoblasts in vitro can be used to study chemical toxicity and the molecular mechanisms thereof. Tobacco-induced oxidative stress disrupts osteogenic differentiation in human embryonic stem cells.

Usage: Immunocytochemistry

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Suarez AN, Hsu TM, Liu CM, Noble EE, Cortella AM, Nakamoto EM, Hahn JD, de Lartigue G, Kanoski SE (2018) Gut vagal sensory signaling regulates hippocampus function through multi-order pathways. Nat Commun 9(1):2181. doi: 10.1038/s41467-018-04639-1

Objective: To determine the  endogenous relevance of GIderived vagal HPC communication.

Summary: Endogenous derived vagal sensory signaling promotes HPC-dependent memory function via a multi-order brainstem–septal pathway, thereby identifying a previously unknown role for the gut–brain axis in memory control.

Usage: A 1-µl volume of CCK-SAP (250 ng/µl) or control Saporin (250 ng/µl) was injected at two sites: 0.5 µl rostral and 0.5 µl caudal to the laryngeal nerve branch.

Related Products: CCK-SAP (Cat. #IT-31), Saporin (Cat. #PR-01)

Sakamoto A, Kato K, Hasegawa T, Ikeda S (2018) An agonistic antibody to EPHA2 exhibits antitumor effects on human melanoma cells. Anticancer Res 38:3273-3282. doi: 10.21873/anticanres.12592

Objective: Investigate the therapeutic potential of antibody to EPHA2 against melanoma in vitro.

Summary: Observations indicate a promising role for EPHA2 as a target in antibody treatments for melanoma, and demonstrate the potential therapeutic effects of an agonistic antibody to EPHA2.

Usage: A375 cells were plated into a flat-bottom, 96-well plate (2,000 cells per well) and incubated for 4 days at 37˚C. Cell suspension included different concentrations of Mab-ZAP, along with either anti-EPHA2 mAb (SHM16, SHM17, or SHM20 at 2 μg/ml final concentration), or a control IgG1 mAb (2 μg/ml final concentration).

Related Products: Mab-ZAP (Cat. #IT-04)