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Focused ultrasound-induced blood-brain barrier opening improves adult hippocampal neurogenesis and cognitive function in a cholinergic degeneration dementia rat model.
Shin J, Kong C, Lee J, Choi BY, Sim J, Koh CS, Park M, Na YC, Suh SW, Chang WS, Chang JW (2019) Focused ultrasound-induced blood-brain barrier opening improves adult hippocampal neurogenesis and cognitive function in a cholinergic degeneration dementia rat model. Alzheimers Res Ther 11(1):110. doi: 10.1186/s13195-019-0569-x
Objective: To investigate the decrease of adult hippocampal neurogenesis (AHN) in Alzheimer’s disease (AD).
Summary: This work studied the effect of FUS on AHN in a cholinergic degeneration rat model of dementia.
Usage: 192-IgG-SAP was injected bilaterally into the lateral ventricle (4 μl at a concentration of 0.63 μg/μl at a rate of 1 μl/min).
Related Products: 192-IgG-SAP (Cat. #IT-01)
A flow cytometric method to quantify the endosomal escape of a protein toxin to the cytosol of target cells
Wensley HJ, Johnston DA, Smith WS, Holmes SE, Flavell SU, Flavell DJ (2019) A flow cytometric method to quantify the endosomal escape of a protein toxin to the cytosol of target cells. Pharm Res 37(1):16. doi: 10.1007/s11095-019-2725-1 PMID: 31873810
Chlorotoxin conjugated with saporin reduces viability of ML-1 thyroid cancer cells in vitro.
Rizvanovic H, Pinheiro AD, Kim K, Thomas J (2019) Chlorotoxin conjugated with saporin reduces viability of ML-1 thyroid cancer cells in vitro. bioRxiv 885483. doi: 10.1101/2019.12.20.885483
Objective: To determine whether Chlorotoxin-conjugated Saporin (CTX-SAP) would inhibit the growth of aggressive thyroid cancer cell lines expressing MMP-2.
Summary: This in vitro study demonstrated the efficacy of a CTX-SAP conjugate in reducing the viability of ML-1 thyroid cancer cells in a dose dependent manner.
Usage: There was a statistically significant reduction in cell viability with increasing concentrations of the CTX-SAP conjugate (biotinylated Chlorotoxin mixed with Streptavidin-ZAP). The cell viability of ML-1 cells was decreased by 49.77% with the treatment of 600 nM of CTX-SAP (F=44.24). Unconjugated Chlorotoxin or Saporin had no significant effect on cell viability a using similar assay.
Related Products: Streptavidin-ZAP (Cat. #IT-27), Saporin (Cat. #PR-01), Chlorotoxin-SAP (Cat. #IT-86)
Chronic treatment with apelin, losartan and their combination reduces myocardial infarct size and improves cardiac mechanical function
Abbasloo E, Najafipour H, Vakili A (2020) Chronic treatment with apelin, losartan and their combination reduces myocardial infarct size and improves cardiac mechanical function. Clin Exp Pharmacol Physiol 47:393-402. doi: 10.1111/1440-1681.13195 PMID: 31630435
Objective: To determine the effect of chronic pretreatment with apelin, losartan and their combination on ischemia-reperfusion (IR) injury in the isolated perfused rat heart and on the expression of apelin-13 receptor (APJ) and angiotensin type 1 receptor (AT1R) in the myocardium.
Summary: Chronic pretreatment with apelin along with AT1R antagonist had more protective effects against IR injury.
Usage: 1:10000
Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)
Adipose-derived stem cells embedded in platelet-rich plasma scaffolds improve the texture of skin grafts in a rat full-thickness wound model
Gao Y, Gao B, Zhu H, Yu Q, Xie F, Chen C, Li Q (2019) Adipose-derived stem cells embedded in platelet-rich plasma scaffolds improve the texture of skin grafts in a rat full-thickness wound model. Burns 46(2):377-385. doi: 10.1016/j.burns.2019.07.041 PMID: 31852618
Usage: western (1:500)
Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)
PMP22 regulates lipid metabolism and cholesterol trafficking: The implication for hereditary peripheral neuropathies
Zhou Y (2019) PMP22 regulates lipid metabolism and cholesterol trafficking: The implication for hereditary peripheral neuropathies. University of Florida Thesis.
Objective: To study the extent of lipid abnormalities and to examine the underlying mechanisms, they determined the expression of lipid-related molecules, and studied morphological and functional alterations in the nerve, liver, and cultured cells from PMP22 KO mice.
Summary: The study reveals a novel role of PMP22 in regulating lipid metabolism and cholesterol trafficking, provide critical information for understanding the pathology of peripheral neuropathies, and shed light on the potential therapy for PMP22-linked neuropathies.
Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Development of novel therapies for marfan syndrome using a human iPSC-disease model
McNamara M (2019) Development of novel therapies for marfan syndrome using a human iPSC-disease model. University of Cambridge Thesis. doi: 10.17863/CAM.38053
Objective: To address different strategies that could potentially be developed into treatments of Marfan Syndrome (MFS) for the clinic.
Summary: Preliminary results show that their disease model exhibits reduced GSK3β protein expression despite GSK3β inhibition proving to be beneficial in restoring some of the phenotypic features. Further experimental efforts need to be effectuated to harness precisely the GSK3β-dependent signaling pathways that govern the phenotypic abnormalities and characterize their MFS disease model.
Usage: immunocytochemistry (1:500)
Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Contribution of the retrotrapezoid nucleus and carotid bodies to hypercapnia- and hypoxia-induced arousal from sleep.
Souza GMPR, Stornetta RL, Stornetta DS, Abbott SBG, Guyenet PG (2019) Contribution of the retrotrapezoid nucleus and carotid bodies to hypercapnia- and hypoxia-induced arousal from sleep. J Neurosci 39(49):9725-9737. doi: 10.1523/JNEUROSCI.1268-19.2019
Objective: To examine the contribution of two lower brainstem nuclei that could be implicated in CO2 and hypoxia-induced arousal.
Summary: RTN, a brainstem nucleus that mediates the effect of brain acidification on breathing, also contributes to arousal elicited by CO2 but not hypoxia.
Usage: To ablate the retrotrapezoid nucleus (RTN), SSP-SAP was administered (2.4 ng) through bilateral injections via a dorsal craniotomy.
Related Products: SSP-SAP (Cat. #IT-11)
Attenuation of angiotensin II–induced hypertension in BubR1 low‐expression mice via repression of angiotensin II receptor 1 overexpression.
Aoyagi Y, Furuyama T, Inoue K, Matsuda D, Matsubara Y, Okahara A, Ago T, Nakashima Y, Mori M, Matsumoto T (2019) Attenuation of angiotensin II–induced hypertension in BubR1 low‐expression mice via repression of angiotensin II receptor 1 overexpression. J Am Heart Assoc 8(23):e011911. doi: 10.1161/JAHA.118.011911 PMID: 31787052
Usage: western blot
Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)
Evidence that the LH surge in ewes involves both neurokinin B–dependent and –independent actions of kisspeptin.
Goodman RL, He W, Lopez JA, Bedenbaugh MN, McCosh RB, Bowdridge EC, Coolen LM, Lehman MN, Hileman SM (2019) Evidence that the LH surge in ewes involves both neurokinin B–dependent and –independent actions of kisspeptin. Endocrinology 160(12):2990-3000. doi: 10.1210/en.2019-00597
Objective: To determine if NKB is involved in the RCh of the ewe in the LH surge.
Summary: NKB signaling in the RCh increases kisspeptin levels critical for the full amplitude of the LH surge in the ewe, but kisspeptin release occurs independently of retrochiamatic area (RCh) input at the onset of the surge to initiate GnRH secretion.
Usage: Bilaterial injections in the RCh of either NK3-SAP or Blank-SAP.
Related Products: NKB-SAP (Cat. #IT-63), Blank-SAP (Cat. #IT-21)