References

Chen XJ, Sun YG (2020) Central circuit mechanisms of itch. Nat Commun 11:3052. doi: 10.1038/s41467-020-16859-5

Summary: The authors summarize the progress in elucidating the neural circuit mechanism of itch at spinal and supraspinal levels.

Related Products: SSP-SAP (Cat. #IT-11)

Kiguchi N, Uta D, Ding H, Uchida H, Saika F, Matsuzaki S, Fukazawa Y, Abe M, Sakimura K, Ko MC, Kishioka S (2020) GRP receptor and AMPA receptor cooperatively regulate itch-responsive neurons in the spinal dorsal horn. Neuropharmacology 170:108025. doi: 10.1016/j.neuropharm.2020.108025

Objective: To investigate the mechanisms for the activation of itch-responsive GRPR+ neurons in the spinal dorsal horn (SDH).

Summary: These findings demonstrate that GRP and glutamate cooperatively regulate GRPR+ AMPAR+ neurons in SDH, mediating itch sensation. GRP–GRPR and the glutamate–AMPAR system may play pivotal roles in the spinal transmission of itch in rodents and nonhuman primates.

Usage: Bombesin-SAP and Blank-SAP were administered i.t. (5 μg/5 μl).

Related Products: Bombesin-SAP (Cat. #IT-40), Blank-SAP (Cat. #IT-21)

Zhao Y, Ge X, Yu H, Kuil LE, Alves MM, Tian D, Huang Q, Chen X, Hofstra RMW, Gao Y (2020) Inhibition of ROCK signaling pathway accelerates enteric neural crest cell-based therapy after transplantation in a rat hypoganglionic model. Neurogastroenterol Motil 32(9):e13895. doi: 10.1111/nmo.13895 PMID: 32515097

Objective: To test the hypothesis that modulation of extracellular factors during transplantation could promote enteric neural crest cells (ENCCs) proliferation and migration, leading to increased transplantation efficiency.

Summary: Since the RhoA/ROCK pathway is highly involved in cytoskeletal dynamics and neurite growth, this study explored the effect of inhibition of this pathway to improve the success of ENCCs transplantation. inhibition of ROCK increased viable cell number, differentiation, and migration of ENCCs in vitro. Moreover, transplantation of labeled ENCCs into the hypoganglionic model showed enhanced distribution of grafted ENCCs, upon treatment with ROCK inhibitor.

Usage: Immunofluorescence (1:200); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Cui S-Y, Huang Y-L, Cui X-Y, Zhao H-L, Hu X, Liu Y-T, Qin Y, Kurban N, Zhang Y-H (2020) A common neuronal mechanism of hypertension and sleep disturbances in spontaneously hypertensive rats: Role of orexinergic neurons. Prog Neuropsychopharmacol Biol Psychiatry 100:109902. doi: 10.1016/j.pnpbp.2020.109902

Objective: To investigate dynamic changes in sleep patterns during the development of hypertension.

Summary: Although the correlation between sleep disturbances and hypertension is very complex, common mechanisms may underlie these comorbidities.

Usage: Orexin-B-SAP (HCRT2-SAP) was administered in two injections/side (100 and 200 ng/0.5 μl/injection).

Related Products: Orexin-B-SAP (Cat. #IT-20)

Haigh JL , Williamson DJ , Poole E , Guo Y , Zhou D , Webb ME , Deuchars SA , Deuchars J , Turnbull WB (2020) A versatile cholera toxin conjugate for neuronal targeting and tracing. Chem Commun (Camb) 56(45:6098-6101. doi: 10.1039/d0cc01085e PMID: 32355935

Related Products: Cholera Toxin B, Recombinant (Cat. #PR-14)

Driessen AK, McGovern AE, Behrens R, Moe AAK, Farrell MJ, Mazzone SB (2020) A role for neurokinin 1 receptor expressing neurons in the paratrigeminal nucleus in bradykinin-evoked cough in guinea-pigs. J Physiol 598(11):2257-2275. doi: 10.1113/JP279644

Objective: This study aimed to assess the involvement of paratrigeminal neurokinin 1 receptor neurons in the regulation of cough, breathing and airway defensive responses.

Summary: These findings warrant further investigations into targeting the jugular ganglia and paratrigeminal nucleus as a therapy for treating cough in disease.

Usage: Targeted toxin lesions across three sites of the paratrigeminal nucleus (200nl per injection site).

Related Products: SSP-SAP (Cat. #IT-11)

Trias E, Kovacs M, King PH, Si Y, Kwon Y, Varela V, Ibarburu S, Moura IC, Hermine O, Beckman JS, Barbeito L (2020) Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis. Glia 68(6):1165-1181. doi: 10.1002/glia.23768 PMID: 31859421

Objective: To investigate the pathogenic significance of denervated Schwann cells (SCs) accumulating following impaired axonal growth in amyotrophic lateral sclerosis (ALS).

Summary: There is strong evidence for a previously unknown inflammatory mechanism triggered by SCs in ALS peripheral nerves that has broad application in developing novel therapies.

Usage: Immunohistochemistry (1:250); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Jani MS, Zou J, Veetil AT, Krishnan Y (2020) A DNA-based fluorescent probe maps NOS3 activity with subcellular spatial resolution. Nat Chem Biol 16:660–666. doi: 10.1038/s41589-020-0491-3 PMID: 32152543

Objective: Usage of novel fluorescent DNA-based probe technology to map activities of endogenous NOS3 in live cells provides a platform to discover selective regulators of NOS3 in PM and TGN.

Summary: Targeting of plasma membrane (PM) and trans-Golgi network (TGN) shows that NOS3 is tenfold less active at the Golgi but its activity is essential for the structural integrity of the Golgi.

Usage: immunofluorescence

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Jia M (2020) Vascular-cognitive impairment after chronic high-thoracic spinal cord injury. University of British Columbia, Experimental Medicine Thesis. PMID: 0

Objective: To investigate whether chronic disruption of cardiovascular control following experimental spinal cord injury (SCI) results in cerebrovascular decline and vascular cognitive impairment.

Summary: Chronic experimental SCI is associated with structural alteration and endothelial dysfunction in cerebral arteries that likely contributes to significantly reduced CBF and vascular cognitive impairment.

Usage: Immunofluorescence (1:600)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Xiao D, Deng Q, Guo Y, Huang X, Zou M, Zhong J, Rao P, Xu Z, Liu Y, Hu Y, Shen Y, Jin K, Xiang M (2020) Generation of self-organized sensory ganglion organoids and retinal ganglion cells from fibroblasts. Sci Adv 6(22):eaaz5858. doi: 10.1126/sciadv.aaz5858 PMID: 32523990

Objective: To examine whether sensory ganglion (SG) organoids can be reprogrammed by transcription factors (TFs) into sensory ganglion (SG) neurons.

Summary: This study identifies a combination of triple TFs Ascl1, Brn3b/3a, and Isl1 (ABI) as an efficient means to reprogram mouse and human fibroblasts into self-organized and networked induced SG (iSG) organoids. iSG organoids may serve as a model to decipher the pathogenesis of sensorineural diseases and screen effective drugs and a source for cell replacement therapy.

Usage: Immunohistochemistry, Immunocytochemistry (1:500); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)