References

Vasquez JH, Borniger JC (2020) Neuroendocrine and behavioral consequences of hyperglycemia in cancer. Endocrinology 161(5):bqaa047. doi: 10.1210/endocr/bqaa047 PMID: 32193527

Summary: Ablation of norepinephrine containing projections to the arcuate (via Anti-DBH-SAP injections) alters AgRP and neuropeptide (NPY) concentrations, leading to impairments in hypoglycemic (glucoprivic) or ghrelin-induced feeding.

Usage: Anti-DBH-SAP (bilateral 42-ng intracranial injections) was used in rats to investigate the role of hindbrain catecholamine afferents in increased ARC NPY and AgRP gene expression.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

See Also:

• Fraley GS et al. Immunolesion of norepinephrine and epinephrine afferents to medial hypothalamus alters basal and 2-deoxy-D-glucose-induced neuropeptide Y and agouti gene-related protein messenger ribonucleic acid expression in the arcuate nucleus. Endocrinology 144(1):75-83, 2003.

Harris RBS (2020) Loss of leptin receptor-expressing cells in the hindbrain decreases forebrain leptin sensitivity. Am J Physiol Endocrinol Metab 318(5):E806-E816. doi: 10.1152/ajpendo.00020.2020

Objective: This study tested whether loss of hindbrain leptin receptor signaling changed sensitivity to forebrain leptin.

Summary: Loss of VMH (ventromedial nucleus of hippocampus) leptin receptor-expressing cells prevents weight loss. The integrated response between the hindbrain and forebrain is heavily dependent upon leptin activity in the VMH.

Usage: To test forebrain leptin sensitivity Leptin-SAP and Blank-SAP rats received third ventricle injections of 0, 0.05, 0.1, 0.25 or 0.5 μg leptin.

Related Products: Leptin-SAP (Cat. #IT-47), Blank-SAP (Cat. #IT-21)

Wang X, Meng H, Wang Q, Shao M, Lu W, Chen X, Jiang Y, Li C, Wang Y, Tu P (2020) Baoyuan decoction ameliorates apoptosis via AT1-CARP signaling pathway in H9C2 cells and heart failure post-acute myocardial infarction rats. J Ethnopharmacol 252:112536. doi: 10.1016/j.jep.2019.112536 PMID: 31931161

Objective: To elucidate whether Baoyuan decoction (BYD) ameliorates apoptosis to protect against heart failure via the AT1-Cardiac ankyrin repeat protein (CARP) signaling pathway.

Summary: BYD improves cardiac function and protects against cardiomyocytes injury by inhibiting apoptosis via regulating the AT1-CARP signaling pathway.

Usage: Western blot (1:1000)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Hasegawa H, Selway R, Gnoni V, Beniczky S, Williams SCR, Kryger M, Ferini-Strambi L, Goadsby P, Leschziner GD, Ashkan K, Rosenzweig I (2020) The subcortical belly of sleep: New possibilities in neuromodulation of basal ganglia?. Sleep Med Rev 52:101317. doi: 10.1016/j.smrv.2020.101317

Summary: Complete BF lesion with OX-SAP leads to coma-like state and flat EEG, reduced Fos in cerebral cortex (but high Fos in brainstem, thalamus, hypothalamus) selective (cholinergic or non-cholinergic) lesion does not have this effect.

Usage: Lesions of the basal forebrain were done by injecting a 0.1% solution of Orexin-SAP at four different sites.

See: Fuller P et al. Reassessment of the structural basis of the ascending arousal system. J Comp Neurol 519(5):933-956, 2011.

Related Products: Orexin-B-SAP (Cat. #IT-20)

Moshirpour M, Nakashima AS, Sehn N, Smith VM, Thackray SE, Dyck RH, Antle MC (2020) Examination of zinc in the circadian system. Neuroscience 432:15-29. doi: 10.1016/j.neuroscience.2020.02.016 PMID: 32087262

Objective: To examine the anatomical and functional aspects of zinc in the circadian system.

Summary: Neither enhancement nor chelation of free zinc at either the SCN or IGL altered circadian responses to phase-shifting light in hamsters.

Usage: Retinal immunohistochemistry (1:5000) included a 20-min wash in 4% PFA prior to initiation of the IHC protocol.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Ma YP, Yang Y, Jiang SM, Liu L, Zhang Z, Wang YN, Zou GM, Li WG (2020) Angiotensin II type 1 receptor blockers favorably affect renal angiotensin II and MAS receptor expression in patients with diabetic nephropathy. J Renin Angiotensin Aldosterone Syst 21(2):1470320320919607. doi: 10.1177/1470320320919607 PMID: 32370637

Objective: To assess the renal expression of angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), and MAS receptor in human type 2 diabetic nephropathy (DN).

Summary: Renal expression levels of AT1R increase during the early stages of DN, ARB reducing AT1R while increasing MAS receptor. Therefore, ARB should be used as soon as possible in patients with DN.

Usage: Immunohistochemistry

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Zhou G, Li J, Zeng T, Yang P, Li A (2020) The regulation effect of WNT-RAS signaling in hypothalamic paraventricular nucleus on renal fibrosis. J Nephrol 33(2):289-297. doi: 10.1007/s40620-019-00637-8 PMID: 31392659

Objective: To investigate whether abnormal expression of central wnt/β-catenin signaling and renin-angiotensin system (WNT-RAS signaling) was tightly involved in chronic kidney disease (CKD).

Summary: These data suggest that the central WNT-RAS signaling plays a significant role in the development and progression of CKD.

Usage: immunohistochemistry and western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

El-Ariny AF, El-Garem YF, Eid AA, Donia HM, Aly RG, Abdelmeniem IM (2020) Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status. Andrologia 52(3):e13513. doi: 10.1111/and.13513 PMID: 31989676

Objective: To study the levels of angiotensin II in semen and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients in relation to their fertility status and to evaluate the influence of varicocelectomy on their levels in infertile varicocele patients.

Summary: Dysregulation of angiotensin II and angiotensin II type 2 receptor in varicocele patients may be involved in varicocele-related infertility.

Usage: Immunocytochemistry (1:15)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Thal LB, Kovtun O, Rosenthal SJ (2020) Labeling neuronal proteins with quantum dots for single-molecule imaging. (eds. Fontes A, Santos B). In: Quantum Dots. Methods in Molecular Biology. 2135:169-177. Humana Press, New York, NY. doi: 10.1007/978-1-0716-0463-2_9 PMID: 32246334

Objective: The authors describe how nanometer-sized fluorescent semiconductors called quantum dots (QD) can be used to label neuronal proteins in a single QD imaging format.

Usage: Secondary antibody-conjugated QDs target membrane proteins pre-treated with SERT Mouse Monoclonal.

Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)

Volobueva MN, Dobryakova YV, Manolova AO, Stepanichev MY, Kvichansky AA, Gulyaeva NV, Markevich VA, Bolshakov AP (2020) Intracerebroventricular administration of 192IgG-saporin alters the state of microglia in the neocortex. Neurochem J 14(1):37-42. doi: 10.1134/S1819712420010213

Objective: The effect of intracerebroventricular (icv) immunotoxin administration on the state of microglia in tissues adjacent to the ventricle (striatum and parietal cortex) and remotely located but receiving innervation from the medial septal region and diagonal band of Broca (entorhinal cortex and olfactory bulbs).

Summary: 1.5 months after the administration of immunotoxin, microglia are activated only in the neocortical areas, not in the striatum or olfactory bulbs.

Usage: Injected bilaterally at a dose of 4 μg in each ventricle.

Related Products: 192-IgG-SAP (Cat. #IT-01)