References

Kang H, Wang Y, Zhong X, Yin W, Li F, Jiang J (2021) [Effect of salt intake on residual renal function in rats receiving peritoneal dialysis]. Nan Fang Yi Ke Da Xue Xue Bao 41(2):264-271. doi: 10.12122/j.issn.1673-4254.2021.02.15 PMID: 33624601

Objective: To assess the effect of salt intake on residual renal function in rats and explore the possible mechanism.

Summary: In rats with chronic renal failure, high salt intake promotes the activation of the renal RAS system, increases blood pressure, and aggravates renal fibrosis to accelerate the decline of residual renal function, and peritoneal dialysis partially reduces the damage of residual renal function induced by high-salt diets by removing excessive sodium.

Usage: Western Blot.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Chen L, Ke Y, Ma H, Gao L, Zhou Y, Zhu H, Liu H, Zhang F, Zhou W (2021) Fluoxetine and ketamine reverse the depressive but not anxiety behavior induced by lesion of cholinergic neurons in the horizontal limb of the diagonal band of broca in male rat. Front Behav Neurosci 15:602708. doi: 10.3389/fnbeh.2021.602708

Summary: A lesion of horizontal limb of the diagonal band of Broca (HDB) cholinergic neurons and followed hippocampus damage may be involved in the pathogenesis of depression.

Usage: Injections of 192-IgG-SAP were made bilaterally into the HDB in a volume of 0.5 µL per side with a concentration of 0.5 µg/µL.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Li Y, Lv S, Yuan H, Ye G, Mu W, Fu Y, Zhang X, Feng Z, He Y, Chen W (2021) Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors. Advanced Functional Materials 31(16):2010215. doi: 10.1002/adfm.202010215

Objective: Regeneration efficiency and behavior of peripheral nerves are compared under three treatment strategies: 1) transplantation of Schwann cell progenitors induced from purified neural crest stem cells; 2) implantation of a multiscale scaffold based on high-resolution 3D printing; and 3) implantation of this bionic scaffold loading Schwann cell progenitors.

Summary: Implantation of multiscale scaffolds preloaded with neural crest stem cell derived Schwann cell progenitors is the best strategy to promote peripheral nerve regeneration with improved anatomy and function among the three different strategies.

Usage: Immunostaining (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Koeniger T, Bell L, Mifka A, Enders M, Hautmann V, Mekala SR, Kirchner P, Ekici AB, Schulz C, Wörsdörfer P, Mencl S, Kleinschnitz C, Ergün S, Kuerten S (2021) Bone marrow-derived myeloid progenitors in the leptomeninges of adult mice. Stem Cells 39(2):227-239. doi: 10.1002/stem.3311

Summary: This report confirms the presence of myeloid progenitors at the meningeal border of the brain and lays the foundation to unravel their possible functions in CNS surveillance and local immune cell production. Compared to bone marrow transfer after whole-body irradiation, chimerism developed more slowly in the CD45-SAP (biotinylated anti-CD45 mixed with Streptavidin-ZAP) model and only reached around 50% in the blood myeloid compartment 15 weeks after transplantation.

See: Palchaudhuri R et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol 34:738-745, 2016.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Meng QT, Liu XY, Liu XT, Barry DM, Jin H, Sun Y, Yang Q, Wan L, Jin JH, Shen kF, Munanairi A, Kim R, Yin J, Tao A, Chen ZF (2021) BNP facilitates NMB-mediated histaminergic itch via NPRC-NMBR crosstalk. bioRxiv 2021.01.26.428310. doi: 10.1101/2021.01.26.428310

Related Products: Nppb-SAP (Cat. #IT-69), Blank-SAP (Cat. #IT-21)

Marquez J, Dong J, Dong C, Tian C, Serrero G (2021) Identification of prostaglandin F2 receptor negative regulator (PTGFRN) as an internalizable target in cancer cells for antibody-drug conjugate development. PLoS One 16(1):e0246197. doi: 10.1371/journal.pone.0246197

Summary: PTGFRN is a cell-surface protein that is upregulated in certain cancer types, including head and neck and, notably, pediatric medulloblastoma, an aggressive cancer with limited therapeutic options. With the selection of the mouse monoclonal antibody 33B7, the authors identified PTGFRN as a potential therapy target, and show that it is internalized by incubation with 33B7. Purified 33B7 antibody was sent to Advanced Targeting Systems where saporin was directly conjugated to the Fc region of 33B7 using their proprietary cleavable linker.

Usage: In a 96-well plate, 2000 cells/well were plated in triplicate in 100 μL of DMEM/F12 medium supplemented with 2.5% FBS, 0.4 ug/ml 33B7 antibody, and 0.9ug/ml of Fab-ZAP mouse. As an isotype control, cells were incubated with mouse Fab IgG-SAP as control (instead of 33B7) and Fab-ZAP.

Related Products: Fab-ZAP mouse (Cat. #IT-48), Fab IgG-SAP (Cat. #IT-67), Custom Conjugates

Nazmi A, Griffin EW, Field RH, Doyle S, Hennessy E, O’Donnell M, Rehill A, McCarthy A, Healy D, Doran MM, Lowry JP, Cunningham C (2021) Cholinergic signalling in the forebrain controls microglial phenotype and responses to systemic inflammation. bioRxiv 2021.01.18.427123. doi: 10.1101/2021.01.18.427123

Related Products: mu p75-SAP (Cat. #IT-16)

Mule SN, Manchola NC, de Oliveira GS, Pereira M, Magalhães RDM, Teixeira AA, Colli W, Alves MJM, Palmisano G (2021) Proteome-wide modulation of S-nitrosylation in Trypanosoma cruzi trypomastigotes upon interaction with the host extracellular matrix. J Proteomics 231:104020. doi: 10.1016/j.jprot.2020.104020 PMID: 33096306

Objective: To map site-specific S-nitrosylated (SNO) proteins from Trypanosoma cruzi trypomastigotes incubated (MTy) or not (Ty) with extracellular matrix (ECM).

Summary: The results provide the first site-specific characterization of S-nitrosylated proteins in T. cruzi and their modulation upon ECM incubation before infection of the mammalian hosts. The reduction of S-nitrosylation upon incubation with ECM, associated with a rewiring of the subcellular distribution and intracellular signaling pathways, was confirmed.

Usage: Indirect Immunofluorescence Assay (1:250)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Moschonas EH, Leary JB, Memarzadeh K, Bou-Abboud CE, Folweiler KA, Monaco CM, Bondi CO (2021) Disruption of basal forebrain cholinergic neurons after traumatic brain injury does not compromise environmental enrichment-mediated cognitive benefits. Brain Res 1751:147175. doi: 10.1016/j.brainres.2020.147175

Objective: To determine if basal forebrain cholinergic neurons are important mediators of environmental enrichment (EE)-induced benefits after traumatic brain injury.

Summary: These data show that despite significant medial septal ChAT+ cell loss, the EE-mediated benefit in cognitive recovery is not compromised.

Usage: 0.22 μg/1.0 μL 192-IgG-SAP was infused over 5 min at a rate of 0.2 μL/min.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Oti T, Satoh K, Uta D, Nagafuchi J, Tateishi S, Ueda R, Takanami K, Young LJ, Galione A, Morris JF, Sakamoto T, Sakamoto H (2021) Oxytocin influences male sexual activity via non-synaptic axonal release in the spinal cord. Curr Biol 31(1):103-114.e5. doi: 10.1016/j.cub.2020.09.089

Summary: Oxytocin directly activates SEG (Spinal Ejaculation Generator)/GRP (Gastrin-Releasing Peptide) neurons via OXTRs (Oxytocin Receptors) and influences male sexual function in the rat lumbar spinal cord.

Usage: Oxytocin-SAP (4 or 40 ng) was infused slowly into the L3 and L4 spinal cord. Blank-SAP was used as control.

Related Products: Oxytocin-SAP (Cat. #IT-46), Blank-SAP (Cat. #IT-21)