References

Anacker A, Esser KH, Lenarz T, Paasche G (2022) Purification of fibroblasts from the spiral ganglion. Front Neurol 13:877342. doi: 10.3389/fneur.2022.877342 PMID: 35493807

Objective: To provide a purified preparation of cochlear fibroblasts, which can be used in vitro for further investigations.

Summary: Subcultivation of the primary spiral ganglion cells culture removed the neurons from the culture and increased the fibroblast to glial cell ratio in the preparations. Direct immunolabeling for the Thy1-glycoprotein and the p75NGFR-enabled fluorescence-based cell sorting. This resulted in a cell culture of cochlear fibroblasts with a purity of more than 99%.

Usage: Cell Sorting (1:400)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Mueller M, Thompson R, Osman KL, Andel E, DeJonge CA, Kington S, Stephenson Z, Hamad A, Bunyak F, Nichols NL, Lever TE (2022) Impact of limb phenotype on tongue denervation atrophy, dysphagia penetrance, and survival time in a mouse model of ALS. Dysphagia 37(6):1777-1795. doi: 10.1007/s00455-022-10442-4 PMID: 35426522

Related Products: CTB-SAP (Cat. #IT-14)

See Also:

• Lind LA et al. Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection. Muscle Nerve 63(3):413-420, 2021.

Lee DS, Kim JE (2022) P2X7 receptor augments LPS-induced nitrosative stress by regulating Nrf2 and GSH levels in the mouse hippocampus. Antioxidants (Basel) 11(4):778. doi: 10.3390/antiox11040778 PMID: 35453462

Objective: To elucidate whether P2X7R affects nuclear factor-erythroid 2-related factor 2 (Nrf2) activity/expression and glutathione (GSH) synthesis under nitrosative stress in response to lipopolysaccharide (LPS)-induced neuroinflammation.

Summary: The findings indicate that P2X7R may augment LPS-induced neuroinflammation by leading to Nrf2 degradation, aberrant glutamate-glutamine cycle, and impaired cystine/cysteine uptake, which would inhibit GSH biosynthesis.

Usage: Immunohistochemistry (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Liu W, Li J, Yang M, Ke X, Dai Y, Lin H, Wang S, Chen L, Tao J (2022) Chemical genetic activation of the cholinergic basal forebrain hippocampal circuit rescues memory loss in Alzheimer’s disease. Alzheimers Res Ther 14(1):53. doi: 10.1186/s13195-022-00994-w

Related Products: 192-IgG-SAP (Cat. #IT-01)

See Also:

• Tomaszewicz M et al. Changes in cortical acetyl-CoA metabolism after selective basal forebrain cholinergic degeneration by 192IgG-saporin. J Neurochem 87(2):318-324, 2003.

Sabbah S, Papendorp C, Behrendt I, Rasras H, Cann J, Leyrer ML, Koplas E, Beltoja M, Etebari C, Gunesch AN, Carrete L, Kim MT, Manoff G, Bhatia-Lin A, Zhao T, Dowling H, Briggman KL, Berson DM (2022) Intrinsically photosensitive retinal ganglion cells evade temporal filtering to encode environmental light intensity. bioRxiv 2022.04.09.487733. doi: 10.1101/2022.04.09.487733

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Nguyen KL, Lamerand SR, Deshpande RP, Taylor BK (2021) Featured Article: Selective ablation of IB4+ primary afferent neurons reduces mechanical and cold hyperalgesia in an EAE mouse model of multiple sclerosis. Targeting Trends 22

Related Products: IB4-SAP (Cat. #IT-10), Blank-SAP (Cat. #IT-21)

Read the featured article in Targeting Trends.

See Also:

• Nguyen KL et al. Contribution of small diameter non-peptidergic primary afferent neurons to central neuropathic pain in a new, more clinically relevant mouse model of multiple sclerosis. Neuroscience 2021 Abstracts P377/07, 2021. Society for Neuroscience, Virtual

Kohno K, Shirasaka R, Yoshihara K, Mikuriya S, Tanaka K, Takanami K, Inoue K, Sakamoto H, Ohkawa Y, Masuda T, Tsuda M (2022) A spinal microglia population involved in remitting and relapsing neuropathic pain. Science 376(6588):86-90. doi: 10.1126/science.abf6805

Objective: To investigate pain recovery mechanisms.

Summary: The authors reveal a mechanism for the remission and recurrence ofneuropathic pain, providing potential targets for therapeutic strategies.

Usage: The dose of CTB-SAP and IB4-SAP was 8 ug/10 uL, diluted in PBS.

Related Products: CTB-SAP (Cat. #IT-14), IB4-SAP (Cat. #IT-10)

Dedoni S, Olianas A, Manconi B, Collu M, Tuveri B, Vincis ME, Olianas MC, Onali P (2022) Upregulation of p75NTR by histone deacetylase inhibitors sensitizes human neuroblastoma cells to targeted immunotoxin-induced apoptosis. Int J Mol Sci 23(7):3849. doi: 10.3390/ijms23073849 PMID: 35409209

Summary: Histone deacetylase (HDAC) inhibitors have been useful chemotherapy agents in the treatment of neuroblastomas, the most frequent solid tumor of childhood. Studies have shown that the expression of the neurotrophin receptor, p75NTR, on human neuroblastoma cells are enhanced after exposure to some HDAC inhibitors. The authors used mu p75-SAP along with two HDAC inhibitors, valproic acid and entinostat, to investigate if they could exploit the upregulation of p75NTR and see if these cells were more susceptible as a target for elimination. The administration of mu p75-SAP only induced cell death in tumors of mice that were pretreated with entinostat

Usage: Cells were treated for 24 hr with entinostat and then exposed to 30 nM of mu p75-SAP for 24 hr.

Related Products: mu p75-SAP (Cat. #IT-16)

Zhai BT, Tian H, Sun J, Zou JB, Zhang XF, Cheng JX, Shi YJ, Fan Y, Guo DY (2022) Urokinase-type plasminogen activator receptor (uPAR) as a therapeutic target in cancer. J Translational Medicine 20:135. doi: 10.1186/s12967-022-03329-3 PMID: 35303878

Summary: The authors describe how a conjugate of Saporin and ATF (Urokinase-type plasminogen activator receptor (uPAR) as a therapeutic target in cancer amino‐terminal fragment of urokinase) exerts antitumor effects by targeting Urokinase-type plasminogen activator receptor (uPAR).

Saha A, Hyzy S, Lamothe T, Hammond K, Clark N, Lanieri L, Bhattarai P, Palchaudhuri R, Gillard GO, Proctor J, Riddle MJ, Panoskaltsis-Mortari A, MacMillan ML, Wagner JE, Kiem HP, Olson LM, Blazar BR (2022) A CD45-targeted antibody-drug conjugate successfully conditions for allogeneic hematopoietic stem cell transplantation in mice. Blood 139(11):1743-1759. doi: 10.1182/blood.2021012366 PMID: 34986233

Objective: To investigate the effectiveness of a CD45-targeted antibody-drug conjugate (ADC) in conditioning for allogeneic hematopoietic stem cell transplantation (HSCT) in mice.

Summary: In this study, researchers evaluated a novel CD45-targeted antibody-drug conjugate as a conditioning regimen for allogeneic hematopoietic stem cell transplantation in mice. The results demonstrated successful conditioning, highlighting the potential of this approach for improving the outcomes of allogeneic HSCT in the future.

Related Products: Anti-CD117-SAP (Cat. #IT-83)

See Also:

• Palchaudhuri R et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol 34:738-745, 2016.
• Czechowicz A et al. Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservation. Nat Commun 10:617, 2019.
• Li Z et al. Hematopoietic chimerism and donor-specific skin allograft tolerance after non-genotoxic CD117 antibody-drug-conjugate conditioning in MHC-mismatched allotransplantation. Nat Commun 10:616, 2019.
• Gao C et al. Nongenotoxic antibody-drug conjugate conditioning enables safe and effective platelet gene therapy of hemophilia A mice. Blood Adv 3(18):2700-2711, 2019.
• Castiello MC et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol 147(1):309-320.e6, 2021.