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Efficient generation of human embryonic stem cell-derived corneal endothelial cells by directed differentiation
McCabe KL, Kunzevitzky NJ, Chiswell BP, Xia X, Goldberg JL, Lanza R (2015) Efficient generation of human embryonic stem cell-derived corneal endothelial cells by directed differentiation. PLoS One 10(12):e0145266. doi: 10.1371/journal.pone.0145266 PMID: 26689688
Usage: For immunostaining of the expression of Zona Occludens protein 1 (ZO-1), von Willebrand factor (vWF), p75/NGFR and CD31, traditional methods were utilized. Anti-NGFr (p75) 1:100.
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Cell-fate determination by ubiquitin-dependent regulation of translation
Werner A, Iwasaki S, McGourty CA, Medina-Ruiz S, Teerikorpi N, Fedrigo I, Ingolia NT, Rape M (2015) Cell-fate determination by ubiquitin-dependent regulation of translation. Nature 525(7570):523-527. doi: 10.1038/nature14978 PMID: 26399832
Usage: Immunofluorescence 1:100
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex.
Bajo V, Leach N, Cordery P, Nodal F, King A (2014) The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex. Eur J Neurosci 40:2922-2940. doi: 10.1111/ejn.12653 PMID: 24945075
Summary: The ferret has become a more common animal model in auditory neuroscience. Unlike rodent models, however, anatomical data describing the organization of the basal forebrain cholinergic system and its projections to the auditory cortex have not been well characterized. Using a variety of methods the authors mapped the architecture of the ferret basal forebrain. IHC was done with several antibodies including anti-ChAT (Cat. #AB-N34AP; 1:1000) and anti-NGFr (Cat. #AB-N07; 1:500). Animals also received 17 μg of ME20.4-SAP (Cat. #IT-15) in a total of 17 injections into the ectosylvian gyrus. The results indicate that acetylcholine is most likely involved in modulation of auditory processing.
Related Products: Choline Acetyltransferase Rabbit Polyclonal, affinity-purified (Cat. #AB-N34AP), NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07), ME20.4-SAP (Cat. #IT-15)
Feeder-free derivation of neural crest progenitor cells from human pluripotent stem cells
Zeltner N, Lafaille FG, Fattahi F, Studer L (2014) Feeder-free derivation of neural crest progenitor cells from human pluripotent stem cells. J Vis Exp 87:51609. doi: 10.3791/51609 PMID: 24893703
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
A robust method to derive functional neural crest cells from human pluripotent stem cells
Kreitzer FR, Salomonis N, Sheehan A, Huang M, Park JS, Spindler MJ, Lizarraga P, Weiss WA, So PL, Conklin BR (2013) A robust method to derive functional neural crest cells from human pluripotent stem cells. Am J Stem Cells 2(2):119-131. PMID: 23862100
Usage: Immunocytochemistry 1:200
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Directed differentiation of human pluripotent cells to neural crest stem cells
Menendez L, Kulik MJ, Page AT, Park SS, Lauderdale JD, Cunningham ML, Dalton S (2013) Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc 8(1):203-212. doi: 10.1038/nprot.2012.156 PMID: 23288320
Summary: This protocol describes in detail how to perform a highly efficient, lineage-specific differentiation of human pluripotent cells to a NCSC fate.
Usage: 0.2 ul per 10^6 cells
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Generation of human melanocytes from induced pluripotent stem cells
Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, Amagai M, Matsuzaki Y, Yamanaka S, Okano H, Kawakami Y (2011) Generation of human melanocytes from induced pluripotent stem cells. PLoS One 6(1):e16182. doi: 10.1371/journal.pone.0016182 PMID: 21249204
Summary: For immunocytochemistry, cells were fixed with PBS containing 4% PFA for 20 min at room temperature. Then, cells were subjected to immunofluorescence staining (1∶100).
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Derivation of neural crest cells from human pluripotent stem cells
Lee G, Chambers SM, Tomishima MJ, Studer L (2010) Derivation of neural crest cells from human pluripotent stem cells. Nat Protoc 5(4):688-701. doi: 10.1038/nprot.2010.35 PMID: 20360764
Summary: Protocols are presented for the purification and propagation of hPSC-NC cells using flow cytometry and defined in vitro culture conditions. This protocol has been validated in multiple independent hESC and hiPSC lines. The average time required for generating purified hPSC-NC precursors using this protocol is 2–5 weeks.
Usage: Neural crest cells (1:200).
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
In vivo labeling of rabbit cholinergic basal forebrain neurons with fluorochromated antibodies.
Hartig W, Varga C, Kacza J, Grosche J, Seeger J, Luiten PG, Brauer K, Harkany T (2002) In vivo labeling of rabbit cholinergic basal forebrain neurons with fluorochromated antibodies. NeuroReport 13(11):1395-1398. doi: 10.1097/00001756-200208070-00009 PMID: 12167760
Summary: To investigate in vivo labeling of p75 low-affinity neurotrophin receptor the authors conjugated Cy3 to ME20.4 (Cat. #AB-N07) and performed either unilateral or bilateral icv injections in rabbits. The antibody labeled only cholinergic neurons demonstrating its potential as a p75 marker.
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Distribution and co-localization of choline acetyltransferase and p75 neurotrophin receptors in the sheep basal forebrain: implications for the use of a specific cholinergic immunotoxin.
Ferreira G, Meurisse M, Tillet Y, Lévy F (2001) Distribution and co-localization of choline acetyltransferase and p75 neurotrophin receptors in the sheep basal forebrain: implications for the use of a specific cholinergic immunotoxin. Neuroscience 104(2):419-439. doi: 10.1016/s0306-4522(01)00075-6 PMID: 11377845
Summary: ME20.4 is a monoclonal antibody (Cat. #AB-N07) that has been shown to bind the p75 receptor in rabbit, sheep, dog, cat, raccoon, pig, and several primate species. Ferreira et al. investigate ME20.4-SAP (bilateral, 150 µl per ventricle, 50-150 µg total; Cat. #IT-15) use in sheep to assess distribution and localization of p75. The authors demonstrate 80-95% loss of basal forebrain cholinergic neurons and acetylcholinesterase-positive fibers in the hippocampus, olfactory bulb, and entorhinal cortex.
Related Products: ME20.4-SAP (Cat. #IT-15), NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)