Yuan H, Hu H, Chen R, Mu W, Wang L, Li Y, Chen Y, Ding X, Xi Y, Mao S, Jiang M, Chen J, He Y, Wang L, Dong Y, Tou J, Chen W (2021) Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10(6):922-938. doi: 10.1002/sctm.20-0469 PMID: 33481357

Objective: To determine whether premigratory NCSCs (pNCSCs) could form enteric neurons and mediate the motility.

Summary: The results show that the pNCSCs that were previously assumed to not be induced by intestinal environment or cues can innervate the intestine and establish neuron-dependent motility.

Usage: Immunochemistry (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Delarue Q, Robac A, Massardier R, Marie JP, Guérout N (2021) Comparison of the effects of two therapeutic strategies based on olfactory ensheathing cell transplantation and repetitive magnetic stimulation after spinal cord injury in female mice. J Neurosci Res 99(7):1835-1849. doi: 10.1002/jnr.24836 PMID: 33960512

Objective: To compare two therapeutic approaches; individual primary olfactory ensheathing cell (OEC) transplantation was compared to repetitive trans-spinal magnetic stimulation (rTSMS). Then, these two therapeutic approaches were combined for tissue repair and functional recovery after spinal cord injury (SCI).

Summary: Results indicate that primary bulbar OEC transplantation and rTSMS treatment act through different mechanisms after SCI to induce functional recovery. The combination of the two therapies does not induce an additional benefit.

Usage: Flow cytometry

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Fang J, Sia J, Soto J, Wang P, Li LK, Hsueh YY, Sun R, Faull KF, Tidball JG, Li S (2021) Skeletal muscle regeneration via the chemical induction and expansion of myogenic stem cells in situ or in vitro. Nat Biomed Eng 5(8):864-879. doi: 10.1038/s41551-021-00696-y PMID: 33737730

Objective: To show that myogenic stem cells expanded from obtainable dermal fibroblasts or skeletal muscle stem cells lead to functional muscle regeneration when transplanted into muscle injuries in adult, aged or dystrophic mice.

Summary: A cocktail of chemicals can selectively and efficiently expand myogenic cells from dermal fibroblast-like cells and skeletal muscle stromal cells. Also, nanoparticle-delivery of the chemical cocktail induces a robust in situ activation and expansion of satellite cells for adult and aged muscle regeneration.

Usage: Immunofluorescence staining (1:100)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Ebadi R, Rabiee F, Kordi-Tamandani D, Nasr-Esfahani MH, Ghaedi K (2021) Fndc5 knockdown significantly decreased the expression of neurotrophins and their respective receptors during neural differentiation of mouse embryonic stem cells. Hum Cell 34(3):847-861. doi: 10.1007/s13577-021-00517-z PMID: 33683654

Objective: To evaluate whether knockdown of Fibronectin type III domain-containing-5 (Fndc5) decreases neural differentiation of mouse embryonic stem cells (mESCs) by affecting the neurotrophins and their receptor expression.

Summary: Results suggest that decreased efficiency of neural differentiation following the reduction of Fndc5 expression could be attributed to decreased levels of NGF and BDNF proteins in addition to their cognate receptors.

Usage: Western blot (1:4000)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Dadashkhan S, Irani S, Bonakdar S, Ghalandari B (2021) P75 and S100 gene expression induced by cell-imprinted substrate and beta-carotene to nerve tissue engineering. Journal of Applied Polymer Science 138(26):50624. doi: 10.1002/app.50624

Objective: Aim to differentiate adipose-derived stem cells (ADSCs) to Schwann cells (SCs), as one of the major cell sources in nerve regeneration.

Summary: Use of synergistic application of imprinting method and beta-carotene BC. Schwann cell imprinted substrates can mimic the morphology and topography of SCs and induce differentiation signals in mesenchymal stem cells. BC stimulates neural differentiation.

Usage: Immunocytochemistry

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Li Y, Lv S, Yuan H, Ye G, Mu W, Fu Y, Zhang X, Feng Z, He Y, Chen W (2021) Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors. Advanced Functional Materials 31(16):2010215. doi: 10.1002/adfm.202010215

Objective: Regeneration efficiency and behavior of peripheral nerves are compared under three treatment strategies: 1) transplantation of Schwann cell progenitors induced from purified neural crest stem cells; 2) implantation of a multiscale scaffold based on high-resolution 3D printing; and 3) implantation of this bionic scaffold loading Schwann cell progenitors.

Summary: Implantation of multiscale scaffolds preloaded with neural crest stem cell derived Schwann cell progenitors is the best strategy to promote peripheral nerve regeneration with improved anatomy and function among the three different strategies.

Usage: Immunostaining (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Motohashi T, Kawamura N, Watanabe N, Kitagawa D, Goshima N, Kunisada T (2020) Sox10 functions as an inducer of the direct conversion of keratinocytes into neural crest cells. Stem Cells Dev 29(23):1510-1519. doi: 10.1089/scd.2020.0106 PMID: 33040687

Objective: To investigate whether Sox10 functions in the direct conversion of other somatic cells into neural crest cells (NCCs).

Summary: Results demonstrate that Sox10 functions as an inducer of direct conversion into NCCs in cooperation with the transcription factors involved in NCC generation.

Usage: Flow cytometry and cell sorting

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Wang Z, Wu J, Hu Z, Luo C, Wang P, Zhang Y, Li H (2020) Dexmedetomidine alleviates lipopolysaccharide-induced acute kidney injury by inhibiting p75NTR-mediated oxidative stress and apoptosis. Oxid Med Cell Longev 2020:5454210. doi: 10.1155/2020/5454210 PMID: 33194004

Objective: To study antioxidant effects and the mechanism of Dexmedetomidine (DEX) in an inflammatory proximal tubular epithelial cell model and lipopolysaccharide- (LPS-) induced AKI in mice.

Summary: DEX ameliorated AKI in mice with sepsis by partially reducing oxidative stress and apoptosis through regulation of p75NTR/p38MAPK/JNK signaling pathways.

Usage: Western blot (1:5000); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Cassandras M, Wang C, Kathiriya J, Tsukui T, Matatia P, Matthay M, Wolters P, Molofsky A, Sheppard D, Chapman H, Peng T (2020) Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nat Cell Biol 22(11):1295-1306. doi: 10.1038/s41556-020-00591-9 PMID: 33046884

Objective: To show that Gli1+ mesenchymal stromal cells (MSCs) promote metaplastic differentiation of airway progenitors into KRT5+ basal cells.

Summary: Gli1+ MSCs integrate hedgehog signaling as a rheostat to control BMP activation in the progenitor niche to determine regenerative outcome in fibrosis.

Usage: Histology and Immunofluorescence (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Zhao Y, Ge X, Yu H, Kuil LE, Alves MM, Tian D, Huang Q, Chen X, Hofstra RMW, Gao Y (2020) Inhibition of ROCK signaling pathway accelerates enteric neural crest cell-based therapy after transplantation in a rat hypoganglionic model. Neurogastroenterol Motil 32(9):e13895. doi: 10.1111/nmo.13895 PMID: 32515097

Objective: To test the hypothesis that modulation of extracellular factors during transplantation could promote enteric neural crest cells (ENCCs) proliferation and migration, leading to increased transplantation efficiency.

Summary: Since the RhoA/ROCK pathway is highly involved in cytoskeletal dynamics and neurite growth, this study explored the effect of inhibition of this pathway to improve the success of ENCCs transplantation. inhibition of ROCK increased viable cell number, differentiation, and migration of ENCCs in vitro. Moreover, transplantation of labeled ENCCs into the hypoganglionic model showed enhanced distribution of grafted ENCCs, upon treatment with ROCK inhibitor.

Usage: Immunofluorescence (1:200); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)