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Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects
Yang R, Gao Y, Li H, Huang W, Tu D, Yang M, Liu X, Hong JS, Gao HM (2022) Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects. Cell Rep 40(11):111330. doi: 10.1016/j.celrep.2022.111330 PMID: 36103834
Objective: To demonstrate that S-nitrosylation (SNO) is essential and sufficient for inflammation-elicited HMGB1 secretion.
Summary: Results indicate crucial roles for SNO modification in HMGB1 secretion and HMGB1-Mac1 interaction for inflammatory neurodegeneration, identifying a mechanistic basis for Parkinson’s Disease development.
Usage: Confocal double-label immunofluorescence (1:1000)
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
Sarco(endo)plasmic reticulum Ca2+-ATPase function is impaired in skeletal and cardiac muscles from young DBA/2J mdx mice
Cleverdon REG, Braun JL, Geromella MS, Whitley KC, Marko DM, Hamstra SI, Roy BD, MacPherson REK, Fajardo VA (2022) Sarco(endo)plasmic reticulum Ca2+-ATPase function is impaired in skeletal and cardiac muscles from young DBA/2J mdx mice. iScience 25(9):104972. doi: 10.1016/j.isci.2022.104972 PMID: 36093052
Objective: To investigate whether sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function would differ in muscles from young D2 and C57 mdx mice.
Summary: The study demonstrates that SERCA function is drastically impaired in young D2 mdx mice.
Usage: Western blot
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
Stimulation of Akt phosphorylation and glucose transport by metalloporphyrins with peroxynitrite decomposition catalytic activity
Eccardt AM, Pelzel RJ, Bell TP, Fisher JS (2022) Stimulation of Akt phosphorylation and glucose transport by metalloporphyrins with peroxynitrite decomposition catalytic activity. Catalysts 12(8):849. doi: 10.3390/catal12080849 PMID: 0
Objective: To determine whether a catalytic action of iron porphyrin compounds would be related to their stimulation of insulin signaling and glucose uptake in C2C12 myotubes.
Summary: Findings suggest that iron porphyrin compounds with both peroxynitrite decomposition activity and peroxidase activity can stimulate insulin signaling and glucose transport in skeletal muscle cells.
Usage: Western blot
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
Characterizing SERCA function in the hippocampal and prefrontal cortex regions of the brain from C57 and D2 mdx mice
Copeland EN (2022) Characterizing SERCA function in the hippocampal and prefrontal cortex regions of the brain from C57 and D2 mdx mice. Brock University St. Catharines, Ontario, Canada Thesis. PMID: 0
Objective: To investigate the differences between two models of Duchenne muscular dystrophy (DMD): D2 mouse model with young mice showing cognitive deficits, and C57, the traditional mouse model.
Summary: Impaired sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function in both C57 and D2 mdx brain – perhaps due to heightened oxidative/nitrosative stress.
Usage: Western blot; NO-L-Cysteine Mouse Monoclonal, Conjugated as a marker of oxidative/nitrosative stress.
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
P2X7 receptor augments LPS-induced nitrosative stress by regulating Nrf2 and GSH levels in the mouse hippocampus
Lee DS, Kim JE (2022) P2X7 receptor augments LPS-induced nitrosative stress by regulating Nrf2 and GSH levels in the mouse hippocampus. Antioxidants (Basel) 11(4):778. doi: 10.3390/antiox11040778 PMID: 35453462
Objective: To elucidate whether P2X7R affects nuclear factor-erythroid 2-related factor 2 (Nrf2) activity/expression and glutathione (GSH) synthesis under nitrosative stress in response to lipopolysaccharide (LPS)-induced neuroinflammation.
Summary: The findings indicate that P2X7R may augment LPS-induced neuroinflammation by leading to Nrf2 degradation, aberrant glutamate-glutamine cycle, and impaired cystine/cysteine uptake, which would inhibit GSH biosynthesis.
Usage: Immunohistochemistry (1:1000)
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
Sarcoplasmic reticulum Ca2+ handling in young DBA/2J vs C57BL/10 mdx models of Duchenne muscular dystrophy
Cleverdon REG, Whitley KC, Marko DM, Hamstra SI, Braun JL, Roy BD, MacPherson REK, Fajardo VA (2021) Sarcoplasmic reticulum Ca2+ handling in young DBA/2J vs C57BL/10 mdx models of Duchenne muscular dystrophy. bioRxiv 2021.10.25.465805. doi: 10.1101/2021.10.25.465805
Objective: To investigate whether sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function would differ in muscles from young D2 and C57 mdx mice.
Summary: The study demonstrates that SERCA function is drastically impaired in young D2 mdx mice.
Usage: Western blot
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
Characterizing SERCA function in murine skeletal muscles after 35-37 days of spaceflight
Braun JL, Geromella MS, Hamstra SI, Messner HN, Fajardo VA (2021) Characterizing SERCA function in murine skeletal muscles after 35-37 days of spaceflight. Int J Mol Sci 22(21):11764. doi: 10.3390/ijms222111764 PMID: 34769190
Objective: To investigate SERCA function, SERCA regulatory protein content, and reactive oxygen/nitrogen species (RONS) protein adduction in murine skeletal muscle after 35–37 days of spaceflight.
Summary: Spaceflight affects SERCA function differently between the soleus and tibialis anterior.
Usage: Western blot (20 mg)
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
Detection of nitric oxide enzymes and its metabolites
de Sanctis JB (2022) Detection of nitric oxide enzymes and its metabolites. (eds. K Agrawal, J Bouchal, V Das, J Drábek, P Dzubák, M Hajdúch, K Koberna, A Ligasová, M Mistrik, JB de Sanctis, J Srovnal). In: Laboratory Techniques in Cellular and Molecular Medicine 243-252. Palacky University. PMID: 0
Objective: To provide a protocol for detection of nitrotyrosine by ELISA.
Summary: Reiss reaction sulfanilic acid (SA) in the presence of acid medium (phosphoric acid) from the diazonium salt interacts with the azo dye agent, N-alpha-naphthyl-ethylenediamine (NAD), to form a pink color which is read at 540 nm.
Usage: Western blot; detection of S-nitrosocysteine
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
Proteome-wide modulation of S-nitrosylation in Trypanosoma cruzi trypomastigotes upon interaction with the host extracellular matrix
Mule SN, Manchola NC, de Oliveira GS, Pereira M, Magalhães RDM, Teixeira AA, Colli W, Alves MJM, Palmisano G (2021) Proteome-wide modulation of S-nitrosylation in Trypanosoma cruzi trypomastigotes upon interaction with the host extracellular matrix. J Proteomics 231:104020. doi: 10.1016/j.jprot.2020.104020 PMID: 33096306
Objective: To map site-specific S-nitrosylated (SNO) proteins from Trypanosoma cruzi trypomastigotes incubated (MTy) or not (Ty) with extracellular matrix (ECM).
Summary: The results provide the first site-specific characterization of S-nitrosylated proteins in T. cruzi and their modulation upon ECM incubation before infection of the mammalian hosts. The reduction of S-nitrosylation upon incubation with ECM, associated with a rewiring of the subcellular distribution and intracellular signaling pathways, was confirmed.
Usage: Indirect Immunofluorescence Assay (1:250)
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats
Uda M, Yoshihara T, Ichinoseki-Sekine N, Baba T, Yoshioka T (2020) Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats. PLoS One 15(12):e0243660. doi: 10.1371/journal.pone.0243660 PMID: 33296434
Objective: To investigate the involvement of neuronal nitric oxide synthase (nNOS) and the PINK1/Parkin pathway in soleus muscle atrophy induced by 14 days of hindlimb unloading (HU) in adult rats.
Summary: Excessive NO is not produced in atrophied soleus muscles despite nNOS accumulation, suggesting that excessive NO dose not mediate in soleus muscle atrophy at least after 14 days of HU.
Usage: Western blot (1:1000)
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)