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A role for thalamic projection GABAergic neurons in circadian responses to light
Brock O, Gelegen CE, Sully P, Salgarella I, Jager P, Menage L, Mehta I, Jęczmień-Łazur J, Djama D, Strother L, Coculla A, Vernon A, Brickley S, Holland P, Cooke S, Delogu A (2022) A role for thalamic projection GABAergic neurons in circadian responses to light. J Neurosci doi: 10.1523/JNEUROSCI.0112-21.2022 PMID: 36280260
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38), Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)
Perinatal correlated retinal activity is required for the wiring of visual axons in non-image forming nuclei
Negueruela S, Morenilla C, Herrera M, Coca Y, Florez-Paz D, López-Cascales MT, Gomis A, Herrera E (2022) Perinatal correlated retinal activity is required for the wiring of visual axons in non-image forming nuclei. bioRxiv 2022.07.27.501692. doi: 10.1101/2022.07.27.501692
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Intrinsically photosensitive retinal ganglion cells evade temporal filtering to encode environmental light intensity
Sabbah S, Papendorp C, Behrendt I, Rasras H, Cann J, Leyrer ML, Koplas E, Beltoja M, Etebari C, Gunesch AN, Carrete L, Kim MT, Manoff G, Bhatia-Lin A, Zhao T, Dowling H, Briggman KL, Berson DM (2022) Intrinsically photosensitive retinal ganglion cells evade temporal filtering to encode environmental light intensity. bioRxiv 2022.04.09.487733. doi: 10.1101/2022.04.09.487733
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Atoh7-independent specification of retinal ganglion cell identity
Brodie-Kommit J, Clark BS, Shi Q, Shiau F, Kim DW, Langel J, Sheely C, Ruzycki PA, Fries M, Javed A, Cayouette M, Schmidt T, Badea T, Glaser T, Zhao H, Singer J, Blackshaw S, Hattar S (2021) Atoh7-independent specification of retinal ganglion cell identity. Sci Adv 7(11):eabe4983. doi: 10.1126/sciadv.abe4983 PMID: 33712461
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Examination of zinc in the circadian system.
Moshirpour M, Nakashima AS, Sehn N, Smith VM, Thackray SE, Dyck RH, Antle MC (2020) Examination of zinc in the circadian system. Neuroscience 432:15-29. doi: 10.1016/j.neuroscience.2020.02.016 PMID: 32087262
Objective: To examine the anatomical and functional aspects of zinc in the circadian system.
Summary: Neither enhancement nor chelation of free zinc at either the SCN or IGL altered circadian responses to phase-shifting light in hamsters.
Usage: Retinal immunohistochemistry (1:5000) included a 20-min wash in 4% PFA prior to initiation of the IHC protocol.
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
nGnG amacrine cells and Brn3b-negative M1 ipRGCs are specifically labeled in the ChAT-ChR2-EYFP mouse.
Cui LJ, Chen WH, Liu AL, Han X, Jiang SX, Yuan F, Zhong YM, Yang XL, Weng SJ (2020) nGnG amacrine cells and Brn3b-negative M1 ipRGCs are specifically labeled in the ChAT-ChR2-EYFP mouse. Invest Ophthalmol Vis Sci 61(2):14. doi: 10.1167/iovs.61.2.14 PMID: 32049344
Summary: The authors speculated that type II cells might be ipRGCs. This was later verified by the strong immunostaining of type II cells in response to the melanopsin antibody UF006 (100%, 141 of 141 cells collected from 5 retinas, Figs. 6B1–B3), which probes multiple ipRGC subtypes.
Usage: Immunostaining 1:10000
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Viability of mouse retinal explant cultures assessed by preservation of functionality and morphology
Alarautalahti V, Ragauskas S, Hakkarainen JJ, Uusitalo-Järvinen H, Uusitalo H, Hyttinen J, Kalesnykas G, Nymark S (2019) Viability of mouse retinal explant cultures assessed by preservation of functionality and morphology. Invest Ophthalmol Vis Sci 60(6):1914-1927. doi: 10.1167/iovs.18-25156 PMID: 31042799
Summary: Organotypic retinal explant cultures have been used to study retinal development, retinal diseases and injuries, drug screening, and retinal stem cell therapies.
Usage: The amount of ganglion cells and melanopsin-expressing intrinsically photosensitive RGCs (ipRGCs) were detected by staining of RNA-binding protein. Melanopsin detection by immunostaining. 1:2500
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Degeneration of ipRGCs in mouse models of huntington’s disease disrupts non-image-forming behaviors before motor impairment.
Lin M-S, Liao P-Y, Chen H-M, Chang C-P, Chen S-K, Chern Y (2019) Degeneration of ipRGCs in mouse models of huntington’s disease disrupts non-image-forming behaviors before motor impairment. J Neurosci 39(8):1505. doi: 10.1523/JNEUROSCI.0571-18.2018 PMID: 30587542
Summary: Results show that M1 ipRGCs were susceptible to the toxicity caused by mutant Huntingtin. The resultant impairment of M1 ipRGCs contributed to the early degeneration of the ipRGC–SCN pathway and disrupted circadian regulation during HD progression.
Usage: Immunostaining (1:3000)
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
The M6 cell: A small-field bistratified photosensitive retinal ganglion cell.
Quattrochi LE, Stabio ME, Kim I, Ilardi MC, Michelle Fogerson P, Leyrer ML, Berson DM (2019) The M6 cell: A small-field bistratified photosensitive retinal ganglion cell. J Comp Neurol 527(1):297-311. doi: 10.1002/cne.24556 PMID: 30311650
Summary: M6 cells express low levels of melanopsin and have correspondingly weak intrinsic light responses.
Usage: In all experiments involving melanopsin immunofluorescence, melanopsin immunodetection was enhanced using tyramide signal amplification coupled with horseradish peroxidase (HRP)-paired with goat anti-rabbit IgG and an Alexa fluorophore. 1:10,000.
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Expression of transcription factors divides retinal ganglion cells into distinct classes.
Sweeney NT, James KN, Nistorica A, Lorig-Roach RM, Feldheim DA (2019) Expression of transcription factors divides retinal ganglion cells into distinct classes. J Comp Neurol 527(1):225-235. doi: 10.1002/cne.24172 PMID: 28078709
Usage: Melanopsin (Opn4) Immunostaining 1:1000
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)