Kim P, Kumar V, Garner N, Jayasingh O, Roman G, Walters S, Vo T, Nguyen Q, Bowles J, Woodruff T, Inder W, Hunt J, Heyde I, Oster H, Rawashdeh O (2025) A systemic clock brake: Period1 stabilizes the circadian network under environmental stress. bioRxiv 2025.06.12.659230. doi: 10.1101/2025.06.12.659230

Objective: To investigate the role of the core circadian clock gene Period1 (Per1) in regulating light-induced circadian realignment and systemic physiological stability across central and peripheral tissues.

Summary: Per1-deficient mice showed accelerated behavioral, hormonal, and metabolic re-entrainment to shifted light-dark cycles, highlighting Per1’s role as a buffer that stabilizes circadian responses. Despite faster adaptation, Per1 deletion compromised SCN network coherence and increased peripheral metabolic phase instability.

Usage: Melanopsin (OPN4) was detected using Anti-Melanopsin (AB-N38) at a 1:2000 dilution to quantify ipRGCs in the retina and confirm that Per1-deficiency did not affect melanopsin-positive cell abundance.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Son S, Beaudoin DL, Hassan AR, Akpo MS, Ichinose T, Garrett AM (2025) A characterization of mouse retinal ganglion cell types labeled with AAV tools. bioRxiv 2025.06.02.657062. doi: 10.1101/2025.06.02.657062

Objective: To characterize the cell-type specificity and functional diversity of retinal ganglion cells (RGCs) labeled by AAV vectors carrying synthetic promoters ProA13 and ProA27 in the mouse retina.

Summary: ProA13 and ProA27 AAV vectors selectively labeled molecularly and morphologically distinct subsets of RGCs, including melanopsin-positive ipRGC subtypes. ProA27 labeled a broader diversity of ipRGCs (M1–M4), while ProA13 primarily labeled M1 cells, enabling analysis of their structural, functional, and projection differences.

Usage: Melanopsin (OPN4) was detected using Anti-Melanopsin (AB-N38) at a 1:5000 dilution to identify and classify ipRGCs in AAV-labeled retinas.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

McLeod CM, Son S, Haque MN, Garrett AM (2025) Reduced neuronal self-avoidance in mouse starburst amacrine cells with only one Pcdhg isoform. bioRxiv 2025.05.29.656828. doi: 10.1101/2025.05.29.656828

Objective: To determine whether the γC4 isoform of the protocadherin-γ (Pcdhg) gene cluster is sufficient to mediate neuronal self-avoidance in starburst amacrine cells (SACs) in the mouse retina.

Summary: While deletion of γC4 or γC5 alone did not impair SAC self-avoidance, mice expressing only γC4 exhibited significant failures in dendritic self-avoidance that were not fully rescued by transgenic overexpression. These findings suggest γC4 is specialized for neuronal survival but insufficient to support self-avoidance on its own.

Usage: Melanopsin (OPN4) was detected using Anti-Melanopsin (AB-N38) at a 1:2000 dilution to label intrinsically photosensitive retinal ganglion cells (ipRGCs) for analysis of retinal cell spacing and mosaic organization.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Semo M, Hughes S, Smyllie NJ, Patton AP, Pothecary CA, Tam SKE, Buckland J, Brown LA (2025) Magnetic fields influence visual responses in mice. bioRxiv 2025.05.12.653455. doi: 10.1101/2025.05.12.653455

Objective: To investigate whether magnetic fields influence mammalian retinal function and to determine the role of cryptochromes in mediating this effect.

Summary: This study demonstrates that magnetic fields modulate neuronal activity in the mouse retina in a light-dependent and cryptochrome-dependent manner. Magnetic fields altered c-Fos expression in melanopsin-positive retinal ganglion cells and influenced retinal circadian rhythms and behavior.

Usage: Melanopsin (OPN4) expression was assessed using Anti-Melanopsin (AB-N38) at a 1:2500 dilution to label ipRGCs during immunohistochemical analysis of retinal responses to light and magnetic fields.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Contreras E, Liang C, Mahoney HL, Javier JL, Luce ML, Labastida Medina K, Bozza T, Schmidt TM (2024) Flp-recombinase mouse line for genetic manipulation of ipRGCs. bioRxiv doi: 10.1101/2024.05.06.592761 PMID: 38766000

Objective: To report the generation and characterization of a new mouse line (Opn4FlpO), in which FlpO is expressed from the Opn4 locus, to manipulate the melanopsin-expressing, intrinsically photosensitive retinal ganglion cells.

Summary: The Opn4FlpO mouse line drives Flp-recombinase expression specifically within ipRGCs, with robust recombination in M1-M3 ipRGC subtypes. This model is a valuable tool for investigating these retinal cells’ physiological and behavioral roles.

Usage: Retinal histology (1:2000 dilution) (AB-N38).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Fitzpatrick MJ, Krizan J, Hsiang JC, Shen N, Kerschensteiner D (2024) A pupillary contrast response in mice and humans: Neural mechanisms and visual functions. Neuron doi: 10.1016/j.neuron.2024.04.012 PMID: 38697114

Objective: To show that temporal contrast drives pupil constriction through a cell-type-specific retinal circuit in mice and humans.

Summary: The pupillary contrast response enhances high spatial frequency contrast in retinal images and improves visual acuity.

Usage: Immunohistochemistry (1:2000) (Cat: AB-N38).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Nord C, Jones I, Garcia-Maestre M, Hägglund AC, Carlsson L (2024) Reduced mTORC1-signaling in progenitor cells leads to retinal lamination deficits. Dev Dyn doi: 10.1002/dvdy.707 PMID: 38546215

Objective: To demonstrate that mTORC1 mediates critical roles during neuronal lamination using the mouse retina as a model system.

Summary: This study establishes a critical role for mTORC1-signaling during retinal lamination and demonstrates that this pathway regulates diverse developmental mechanisms involved in driving the stratified arrangement of neurons during CNS development.

Usage: Immunohistochemistry (AB-N38) (1:1000).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Qian C, Xin Y, Qi C, Wang H, Dong BC, Zack DJ, Blackshaw S, Hattar S, Zhou FQ, Qian J (2024) Intercellular communication atlas reveals Oprm1 as a neuroprotective factor for retinal ganglion cells. Nat Commun 15(1):2206. doi: 10.1038/s41467-024-46428-z PMID: 38467611

Objective: To explore how intercellular communication contributes to retinal ganglion cell (RGC) survival following optic nerve crush based on single-cell RNA-seq analysis.

Summary: The overall scores of the responsive interactions among the retinal cell types correlated with the distress interactions sent from RGCs.

Usage: Immunohistochemistry (1:500; AB-N38).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Maloney R, Quattrochi L, Yoon J, Souza R, Berson D (2024) Efficacy and specificity of melanopsin reporters for retinal ganglion cells. J Comp Neurol 532(2):e25591. doi: 10.1002/cne.25591 PMID: 38375612

Objective: To evaluate the precision and comprehensiveness of various labeling methods for intrinsically photosensitive retinal ganglion cells (ipRGCs).

Summary: The authors provide a comparative analysis of the strengths and limitations of each approach and highlight the need for tailored methods based on specific research applications.

Usage: Immunohistochemistry (1:10,000) (AB-N38).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Matcham AC, Toma K, Tsai NY, Sze CJ, Lin PY, Stewart IF, Duan X (2024) Cadherin-13 Maintains Retinotectal Synapses via Transneuronal Interactions. J Neurosci 44(5):e1310232023. doi: 10.1523/JNEUROSCI.1310-23.2023 PMID: 38123991

Objective: To explore the role of Type II cadherins, particularly Cdh13, in the formation of specific retinotectal synapses.

Summary: Cdh13 is highly expressed in wide-field neurons of the superficial superior colliculus (sSC), and its removal from presynaptic retinal ganglion cells (RGCs) leads to a significant reduction in dendritic spines on postsynaptic wide-field neurons. Unlike αRGCs and On–Off Direction-Selective Ganglion Cells (ooDSGCs), Cdh13-expressing RGCs utilize distinct mechanisms to establish precise retinotectal connections.

Usage: Immunohistochemistry (1:500).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38), Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)