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Selective formation of covalent protein heterodimers with an unnatural amino acid.
Hutchins BM, Kazane SA, Staflin K, Forsyth JS, Felding-Habermann B, Smider VV, Schultz PG (2011) Selective formation of covalent protein heterodimers with an unnatural amino acid. Chem Biol 18(3):299-303. doi: 10.1016/j.chembiol.2011.01.006 PMID: 21439474
Summary: This work demonstrates the creation of a variety of constructs containing specific defined conjugation sites. One use for these molecules is to create homogenous antibody conjugates‚ meaning the properties of these conjugates can be quantitatively evaluated. Having greater control of such conjugations is essential if these types of constructs are to move toward use as therapeutics. The authors created an anti-Her2 Fab-saporin molecule and tested it in vitro. Analysis by western used anti-SAP-HRP (Cat. #AB-15-HRP) to detect the conjugated molecule.
Related Products: Saporin Goat Polyclonal, HRP-labeled (Cat. #AB-15HRP)
Signal peptide-regulated toxicity of a plant ribosome-inactivating protein during cell stress.
Marshall RS, D’Avila F, Di Cola A, Traini R, Spano L, Fabbrini MS, Ceriotti A (2011) Signal peptide-regulated toxicity of a plant ribosome-inactivating protein during cell stress. Plant J 65(2):218-29. doi: 10.1111/j.1365-313X.2010.04413.x PMID: 21223387
Summary: Type I ribosome inactivating proteins (RIPs) are thought to have a role in defending plants against viral or fungal infections. Most type I RIPs have signal peptides for insertion into the endoplasmic reticulum, followed by transportation to a vacuole or the cell wall. The authors examined signal peptide regulation under stress in tobacco plants transfected with saporin. One method of analysis was western blots using anti-saporin (Cat. #AB-15).
Related Products: Saporin Goat Polyclonal (Cat. #AB-15)
Featured Article: Selective deletion of CD8+ T cells by saporin-coupled MHC class I tetramers
Hess PR, Buntzman AS, Murray SL, Young EF, Frelinger JA (2009) Featured Article: Selective deletion of CD8+ T cells by saporin-coupled MHC class I tetramers. Targeting Trends 10(1)
Related Products: Streptavidin-ZAP (Cat. #IT-27), Saporin Goat Polyclonal, affinity-purified FITC-labeled (Cat. #AB-15APFL)
Read the featured article in Targeting Trends.
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Cytotoxic targeting of isolectin IB4-binding sensory neurons.
Vulchanova L, Olson TH, Stone LS, Riedl MS, Elde R, Honda CN (2001) Cytotoxic targeting of isolectin IB4-binding sensory neurons. Neuroscience 108(1):143-155. doi: 10.1016/s0306-4522(01)00377-3 PMID: 11738138
Summary: Vulchanova et al. examine the role of IB4-binding neurons in nociception. IB4-SAP (Cat. #IT-10) was injected into rats (2 µg in left sciatic nerve). The resulting ablation of IB4-binding neurons provides evidence for their role in nociceptive processing and demonstrates a rapid compensatory response to signalling of acute pain.
Related Products: IB4-SAP (Cat. #IT-10), Saporin Goat Polyclonal (Cat. #AB-15), Saporin Goat Polyclonal, HRP-labeled (Cat. #AB-15HRP)
Differential changes in rat cholinergic parameters subsequent to immunotoxic lesion of the basal forebrain nuclei.
Waite JJ, Chen AD (2001) Differential changes in rat cholinergic parameters subsequent to immunotoxic lesion of the basal forebrain nuclei. Brain Res 918:113-120. doi: 10.1016/s0006-8993(01)02968-7 PMID: 11684049
Summary: 192-Saporin (Cat. #IT-01) is used extensively to eliminate the cholinergic neurons of the basal forebrain in rats. Waite and Chen compare the degree of loss between 192-Saporin (6 or 8.2 µg in 10 µl into left lateral ventricle) and control (Saporin, 1.82 µg into left lateral ventricle; Cat. #PR-01) using three methods: Assay of post mortem choline acetyltransferase activity, in vivo microdialysis of extracellular acetylcholine (ACh), and in vivo assessment of the rate of ACh synthesis. The infusion of saporin alone had no effect. After fifteen weeks, the authors report compensation of cholinergic activity in lesioned animals occurs in the hippocampus, but not in the frontal cortex as determined by measurement of the rate of ACh synthesis.
Related Products: 192-IgG-SAP (Cat. #IT-01), Saporin Goat Polyclonal (Cat. #AB-15), Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP), Saporin (Cat. #PR-01)
Hypocretin-2-saporin lesions of the lateral hypothalamus produce narcoleptic-like sleep behavior in the rat.
Gerashchenko D, Kohls MD, Greco M, Waleh NS, Salin-Pascual R, Kilduff TS, Lappi DA, Shiromani PJ (2001) Hypocretin-2-saporin lesions of the lateral hypothalamus produce narcoleptic-like sleep behavior in the rat. J Neurosci 21(18):7273-7283. doi: 10.1523/JNEUROSCI.21-18-07273.2001 PMID: 11549737
Summary: Orexin (also knows as hypocretin) peptides are produced exclusively by neurons in the lateral hypothalamus, however non-specific lesioning in this region has not produced narcoleptic-like sleep. Gerashchenko et al. use orexin-SAP (490 ng/0.5 µl; Cat. #IT-20) to specifically eliminate orexin neurons in rats. The treated rats displayed several sleep disturbances found in narcolepsy, including increased slow-wave sleep, and sleep-onset REM sleep periods. The data suggest that orexin-SAP can be used to create a model for narcolepsy in rats.
Related Products: Orexin-B-SAP (Cat. #IT-20), Saporin Goat Polyclonal, affinity-purified FITC-labeled (Cat. #AB-15APFL), Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP)
Cytosolic immunization allows the expression of preATF-saporin chimeric toxin in eukaryotic cells.
Fabbrini MS, Carpani D, Soria MR, Ceriotti A (2000) Cytosolic immunization allows the expression of preATF-saporin chimeric toxin in eukaryotic cells. FASEB J 14(2):391-398. doi: 10.1096/fasebj.14.2.391 PMID: 10657995
Related Products: Saporin Goat Polyclonal (Cat. #AB-15)
Inhibition of hyperalgesia by ablation of lamina I spinal neurons expressing the substance P receptor.
Mantyh PW, Rogers SD, Honore P, Allen BJ, Ghilardi JR, Li J, Daughters RS, Lappi DA, Wiley RG, Simone DA (1997) Inhibition of hyperalgesia by ablation of lamina I spinal neurons expressing the substance P receptor. Science 278:275-279. doi: 10.1126/science.278.5336.275 PMID: 9323204
Related Products: Saporin (Cat. #PR-01), Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP), Saporin Goat Polyclonal (Cat. #AB-15), Saporin Goat Polyclonal, HRP-labeled (Cat. #AB-15HRP), SP-SAP (Cat. #IT-07), Antibody to NK-1 Receptor (Cat. #AB-N04)
Ribosome-inactivating proteins from plants: present status and future prospects.
Stirpe F, Barbieri L, Battelli MG, Soria M, Lappi DA (1992) Ribosome-inactivating proteins from plants: present status and future prospects. Bio/Technol 10:405-412. doi: 10.1038/nbt0492-405 PMID: 1368484
Related Products: Saporin (Cat. #PR-01), Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP), Saporin Goat Polyclonal (Cat. #AB-15), MonoBiotin-ZAP (Cat. #BT-ZAP)
Characterization of a Saponaria officinalis seed ribosome-inactivating protein: immunoreactivity and sequence homologies.
Lappi DA, Esch FS, Barbieri L, Stirpe F, Soria M (1985) Characterization of a Saponaria officinalis seed ribosome-inactivating protein: immunoreactivity and sequence homologies. Biochem Biophys Res Commun 129:934-942. doi: 10.1016/0006-291x(85)91981-3 PMID: 3925952
Related Products: Saporin (Cat. #PR-01), Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP), Saporin Goat Polyclonal (Cat. #AB-15), MonoBiotin-ZAP (Cat. #BT-ZAP)