FAQ

Frequently asked questions and answers for ATS products and services.

91 entries

Second Immunotoxins

Q: What is a second immunotoxin?
A: ATS’s second immunotoxins are conjugations of a secondary antibody (as of December 2000, either goat anti-mouse IgG or goat anti-rabbit IgG) to the ribosome-inactivating protein, saporin.
Q: How does a second immunotoxin target?
A: The second immunotoxin uses the secondary antibody to “piggyback” onto your primary antibody in order to evaluate the ability of the primary antibody to internalize.
Q: What happens when the second immunotoxin gets inside the cell?
A: If the second immunotoxin is internalized, saporin will inactivate the ribosomes of the cell, thereby causing cell death.
Q: Are there different types of second immunotoxins available?
A: Yes, please see our catalog listing online for a complete list.
Q: What is the ratio of antibody to second immunotoxin for in vitro testing?
A: Both Mab-ZAP and Rab-ZAP have been shown effective in concentrations ranging from 0.5 to 2 moles of primary antibody per mole of second immunotoxin.
See: ZAP Conjugates (Second Immunotoxins)